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The role of heat shock protein 70 on oocyte apoptosis during vitrification

Rimayanti Rimayanti, Aswin Rafif Khairullah, Sri Pantja Madyawati, Fedik Abdul Rantam, Imam Mustofa, Widjiati Widjiati, Pudji Srianto, Adeyinka Oye Akintunde, Bima Putra Pratama, Riza Zainuddin Ahmad, Bantari Wisynu Kusuma Wardhani, Andi Thafida Khalisa, Syahputra Wibowo.


Abstract
The cryopreservation of animal and human oocytes has potential for the development of Assisted Reproductive Technology (ART), especially as bank of oocytes. One of the techniques used for oocyte cryopreservation is vitrification procedure to avoid the critical temperature and reduce cell damage caused by the formation of ice crystals on oocyte cryopreservation. Although Oocyte vitrification method is practical, applicable, efficient, inexpensive, and simple, but still needs further research because of the negative effects caused by temperature stress. Extreme temperature due to temperature changes from hot - cold - hot in the series of vitrification processes need to be aware of damage to the structure of cell proteins and DNA. Extreme temperature will cause stress oocytes. Stress cells increase the need of Heat Shock Protein 70 as a stress protein. The response of Heat Shock Protein 70 (HSP70) inductive or synthesize is thought to be protective mechanism of the cells to stress condition such as an extreme change of temperature. Assessment needs to be done regarding the role of HSP70 in cell protection against the extreme changes of temperature during oocyte vitrification by adding supplementation of HSP70 into the cryoprotectant vitrification. In conclusion, the HSP70 has a a pivotal role in cell protection against stress cells during oocyte vitrification. Additionally, fresh oocytes, in vitro maturated oocytes, and post-warming oocytes exhibit the HSP70 and Cytochrome c expression. Supplementation of HSP70 into vitrification medium expected useful to suppressed the activation of caspase 3, resulted in maintain the viability of oocytes during vitrification and decreased the incidence of apoptosis after warming.

Key words: Apoptosis; Caspase 3; Cytochrome c; In vitro; Oocyte maturation; Endangered species; Extinction risk.


 
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How to Cite this Article
Pubmed Style

Rimayanti R, Khairullah AR, Madyawati SP, Rantam FA, Mustofa I, Widjiati W, Srianto P, Akintunde AO, Pratama BP, Ahmad RZ, Wardhani BWK, Khalisa AT, Wibowo S. The role of heat shock protein 70 on oocyte apoptosis during vitrification. Open Vet. J.. 2025; 15(10): 4847-4864. doi:10.5455/OVJ.2025.v15.i10.5

Web Style

Rimayanti R, Khairullah AR, Madyawati SP, Rantam FA, Mustofa I, Widjiati W, Srianto P, Akintunde AO, Pratama BP, Ahmad RZ, Wardhani BWK, Khalisa AT, Wibowo S. The role of heat shock protein 70 on oocyte apoptosis during vitrification. https://www.openveterinaryjournal.com/?mno=252630 [Access: October 31, 2025]. doi:10.5455/OVJ.2025.v15.i10.5

AMA (American Medical Association) Style

Rimayanti R, Khairullah AR, Madyawati SP, Rantam FA, Mustofa I, Widjiati W, Srianto P, Akintunde AO, Pratama BP, Ahmad RZ, Wardhani BWK, Khalisa AT, Wibowo S. The role of heat shock protein 70 on oocyte apoptosis during vitrification. Open Vet. J.. 2025; 15(10): 4847-4864. doi:10.5455/OVJ.2025.v15.i10.5


Vancouver/ICMJE Style

Rimayanti R, Khairullah AR, Madyawati SP, Rantam FA, Mustofa I, Widjiati W, Srianto P, Akintunde AO, Pratama BP, Ahmad RZ, Wardhani BWK, Khalisa AT, Wibowo S. The role of heat shock protein 70 on oocyte apoptosis during vitrification. Open Vet. J.. (2025), [cited October 31, 2025]; 15(10): 4847-4864. doi:10.5455/OVJ.2025.v15.i10.5


Harvard Style

Rimayanti, R., Khairullah, . A. R., Madyawati, . S. P., Rantam, . F. A., Mustofa, . I., Widjiati, . W., Srianto, . P., Akintunde, . A. O., Pratama, . B. P., Ahmad, . R. Z., Wardhani, . B. W. K., Khalisa, . A. T. & Wibowo, . S. (2025) The role of heat shock protein 70 on oocyte apoptosis during vitrification. Open Vet. J., 15 (10), 4847-4864. doi:10.5455/OVJ.2025.v15.i10.5


Turabian Style

Rimayanti, Rimayanti, Aswin Rafif Khairullah, Sri Pantja Madyawati, Fedik Abdul Rantam, Imam Mustofa, Widjiati Widjiati, Pudji Srianto, Adeyinka Oye Akintunde, Bima Putra Pratama, Riza Zainuddin Ahmad, Bantari Wisynu Kusuma Wardhani, Andi Thafida Khalisa, and Syahputra Wibowo. 2025. The role of heat shock protein 70 on oocyte apoptosis during vitrification. Open Veterinary Journal, 15 (10), 4847-4864. doi:10.5455/OVJ.2025.v15.i10.5


Chicago Style

Rimayanti, Rimayanti, Aswin Rafif Khairullah, Sri Pantja Madyawati, Fedik Abdul Rantam, Imam Mustofa, Widjiati Widjiati, Pudji Srianto, Adeyinka Oye Akintunde, Bima Putra Pratama, Riza Zainuddin Ahmad, Bantari Wisynu Kusuma Wardhani, Andi Thafida Khalisa, and Syahputra Wibowo. "The role of heat shock protein 70 on oocyte apoptosis during vitrification." Open Veterinary Journal 15 (2025), 4847-4864. doi:10.5455/OVJ.2025.v15.i10.5


MLA (The Modern Language Association) Style

Rimayanti, Rimayanti, Aswin Rafif Khairullah, Sri Pantja Madyawati, Fedik Abdul Rantam, Imam Mustofa, Widjiati Widjiati, Pudji Srianto, Adeyinka Oye Akintunde, Bima Putra Pratama, Riza Zainuddin Ahmad, Bantari Wisynu Kusuma Wardhani, Andi Thafida Khalisa, and Syahputra Wibowo. "The role of heat shock protein 70 on oocyte apoptosis during vitrification." Open Veterinary Journal 15.10 (2025), 4847-4864. Print. doi:10.5455/OVJ.2025.v15.i10.5


APA (American Psychological Association) Style

Rimayanti, R., Khairullah, . A. R., Madyawati, . S. P., Rantam, . F. A., Mustofa, . I., Widjiati, . W., Srianto, . P., Akintunde, . A. O., Pratama, . B. P., Ahmad, . R. Z., Wardhani, . B. W. K., Khalisa, . A. T. & Wibowo, . S. (2025) The role of heat shock protein 70 on oocyte apoptosis during vitrification. Open Veterinary Journal, 15 (10), 4847-4864. doi:10.5455/OVJ.2025.v15.i10.5


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