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Research Article


Evaluation of homemade biodesinfectant solution based on Lactobacillus (Lactobacillus casei, Lactobacillus paracasei) and baker’s yeast applied to broiler litter

Roostita L. Balia, Daniswara Danindra Darmesti, Lilis Suryaningsih, Wendry Setiyadi Putranto, Tuti Widjastuti, Ratu Safitri, Eka Wulandari, Gemilang Lara Utama, Muhammad Farid Rizal.


Abstract
Background:
Poultry farming is a rapidly expanding sector that faces numerous challenges related to biosecurity and production efficiency. One major concern is the prevalence of pathogens, such as Escherichia coli, in broiler farm litter, which can lead to increased morbidity and mortality in flocks, ultimately affecting farm productivity.

Aim:
This study aimed to evaluate the efficacy of a farmer’s homemade biodisinfectant solution containing Lactobacillus casei, Lactobacillus paracasei, Saccharomyces cerevisiae, and Eco Enzyme applied to broiler farm litter for reducing Escherichia coli (E. coli) populations and their subsequent effects on broiler production performance.

Methods:
A biodesinfectant was applied to the litter of a commercial broiler farm over a period of 30 days. Oneday- old Ross broilers (900 samples) were fed pre-starter, starter, and finisher diets as controls. The farm consisted of 3 broiler grow-out pens that were used for 3 treatment groups. As the maximum stocking density was restricted to 18 kg/m², the house space is 600 m². The biodisinfectant was applied at two concentrations in the treatment groups: TA (1 ml/100 ml) and TB (1 ml/250 ml) at 2-day intervals. Performance metrics, including FCR, slaughter body weight (BW), mortality rate, and overall health, were monitored. The control group (CON) had the lowest BW (p < 0.05) and the highest mortality rate (p < 0.05) compared with the other treatments.

Results:
Significant E. coli reduction at both treatments (p < 0.05) compared with the control, with no significant adverse effects on broiler performance metrics in every treatment group.

Conclusion:
These findings suggest that biodisinfectants offer a promising, eco-friendly alternative for enhancing both biosecurity and production performance efficiency in broiler poultry farms.

Key words: Biodesinfectant; Lactobacillus casei; Lactobacillus paracasei; Saccharomyces cerevisiae; Broiler farm.


 
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How to Cite this Article
Pubmed Style

Balia RL, Darmesti DD, Suryaningsih L, Putranto WS, Widjastuti T, Safitri R, Wulandari E, Utama GL, Rizal MF. Evaluation of homemade biodesinfectant solution based on Lactobacillus (Lactobacillus casei, Lactobacillus paracasei) and baker’s yeast applied to broiler litter. Open Vet. J.. 2026; 16(3): 1771-1780. doi:10.5455/OVJ.2026.v16.i3.34

Web Style

Balia RL, Darmesti DD, Suryaningsih L, Putranto WS, Widjastuti T, Safitri R, Wulandari E, Utama GL, Rizal MF. Evaluation of homemade biodesinfectant solution based on Lactobacillus (Lactobacillus casei, Lactobacillus paracasei) and baker’s yeast applied to broiler litter. https://www.openveterinaryjournal.com/?mno=273127 [Access: March 31, 2026]. doi:10.5455/OVJ.2026.v16.i3.34

AMA (American Medical Association) Style

Balia RL, Darmesti DD, Suryaningsih L, Putranto WS, Widjastuti T, Safitri R, Wulandari E, Utama GL, Rizal MF. Evaluation of homemade biodesinfectant solution based on Lactobacillus (Lactobacillus casei, Lactobacillus paracasei) and baker’s yeast applied to broiler litter. Open Vet. J.. 2026; 16(3): 1771-1780. doi:10.5455/OVJ.2026.v16.i3.34


Vancouver/ICMJE Style

Balia RL, Darmesti DD, Suryaningsih L, Putranto WS, Widjastuti T, Safitri R, Wulandari E, Utama GL, Rizal MF. Evaluation of homemade biodesinfectant solution based on Lactobacillus (Lactobacillus casei, Lactobacillus paracasei) and baker’s yeast applied to broiler litter. Open Vet. J.. (2026), [cited March 31, 2026]; 16(3): 1771-1780. doi:10.5455/OVJ.2026.v16.i3.34


Harvard Style

Balia, R. L., Darmesti, . D. D., Suryaningsih, . L., Putranto, . W. S., Widjastuti, . T., Safitri, . R., Wulandari, . E., Utama, . G. L. & Rizal, . M. F. (2026) Evaluation of homemade biodesinfectant solution based on Lactobacillus (Lactobacillus casei, Lactobacillus paracasei) and baker’s yeast applied to broiler litter. Open Vet. J., 16 (3), 1771-1780. doi:10.5455/OVJ.2026.v16.i3.34


Turabian Style

Balia, Roostita L., Daniswara Danindra Darmesti, Lilis Suryaningsih, Wendry Setiyadi Putranto, Tuti Widjastuti, Ratu Safitri, Eka Wulandari, Gemilang Lara Utama, and Muhammad Farid Rizal. 2026. Evaluation of homemade biodesinfectant solution based on Lactobacillus (Lactobacillus casei, Lactobacillus paracasei) and baker’s yeast applied to broiler litter. Open Veterinary Journal, 16 (3), 1771-1780. doi:10.5455/OVJ.2026.v16.i3.34


Chicago Style

Balia, Roostita L., Daniswara Danindra Darmesti, Lilis Suryaningsih, Wendry Setiyadi Putranto, Tuti Widjastuti, Ratu Safitri, Eka Wulandari, Gemilang Lara Utama, and Muhammad Farid Rizal. "Evaluation of homemade biodesinfectant solution based on Lactobacillus (Lactobacillus casei, Lactobacillus paracasei) and baker’s yeast applied to broiler litter." Open Veterinary Journal 16 (2026), 1771-1780. doi:10.5455/OVJ.2026.v16.i3.34


MLA (The Modern Language Association) Style

Balia, Roostita L., Daniswara Danindra Darmesti, Lilis Suryaningsih, Wendry Setiyadi Putranto, Tuti Widjastuti, Ratu Safitri, Eka Wulandari, Gemilang Lara Utama, and Muhammad Farid Rizal. "Evaluation of homemade biodesinfectant solution based on Lactobacillus (Lactobacillus casei, Lactobacillus paracasei) and baker’s yeast applied to broiler litter." Open Veterinary Journal 16.3 (2026), 1771-1780. Print. doi:10.5455/OVJ.2026.v16.i3.34


APA (American Psychological Association) Style

Balia, R. L., Darmesti, . D. D., Suryaningsih, . L., Putranto, . W. S., Widjastuti, . T., Safitri, . R., Wulandari, . E., Utama, . G. L. & Rizal, . M. F. (2026) Evaluation of homemade biodesinfectant solution based on Lactobacillus (Lactobacillus casei, Lactobacillus paracasei) and baker’s yeast applied to broiler litter. Open Veterinary Journal, 16 (3), 1771-1780. doi:10.5455/OVJ.2026.v16.i3.34


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