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Research Article


Effectiveness of papaya leaf extract (Carica papaya L.) as an infertility agent on estrus cycle length and expression of estrogen receptor beta on ovaries of female white rats (Rattus norvegicus)

Viski Fitri Hendrawan, Alsya Pritama, Aulia Firmawati, Intan Firdha Olien Noor Al Ichsan, Ertika Fitri Lisnanti.


Abstract
Background:
Natural contraception is an affordable and accessible alternative to synthetic hormonal methods. One strategy for controlling overpopulation involves the use of plant-based agents that possess antifertility properties. Carica papaya (papaya) leaves have garnered scientific interest due to their rich content of bioactive compounds. Flavonoids, alkaloids, and saponins present in papaya leaves are known to influence reproductive physiology by modulating hormonal activity, disrupting follicular development, and altering uterine conditions.

Aim:
This study aimed to investigate the antifertility effects of papaya (Carica papaya L.) leaf extract by evaluating its influence on the length of the estrous cycle and the expression of estrogen receptor beta (ER-β) in the ovaries of female white rats (Rattus norvegicus).

Methods:
This study included six female albino rats (Rattus norvegicus) in the treatment group: negative control, P1 (3.15 mg/kgBW), and P2 (4.72 mg/kgBW) groups. Vaginal swab and Giemsa-stained epithelial histopathology were used to determine the estrous cycle duration. Immunohistochemistry was utilized to observe ovarian ER-β receptors.

Results:
The P2 dosage group demonstrated a 3-day increase in estrus cycle length and a 56.97% decrease in ovarian ER-β expression. This shows that the group can prolong the estrous cycle with a diestrus phase predominance and diminish ovarian ER-β expression, making it an anti-infertility drug.

Conclusion:
The P2 group best prolongs the estrous cycle of female white rats. The expression of ovarian ER-β decreases.

Key words: Antifertility, Extract of papaya leaf, ER-β, Vaginal Swab


 
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How to Cite this Article
Pubmed Style

Hendrawan VF, Pritama A, Firmawati A, , Lisnanti EF. Effectiveness of papaya leaf extract (Carica papaya L.) as an infertility agent on estrus cycle length and expression of estrogen receptor beta on ovaries of female white rats (Rattus norvegicus). Open Vet. J.. 2025; 15(8): 3831-3837. doi:10.5455/OVJ.2025.v15.i8.48

Web Style

Hendrawan VF, Pritama A, Firmawati A, , Lisnanti EF. Effectiveness of papaya leaf extract (Carica papaya L.) as an infertility agent on estrus cycle length and expression of estrogen receptor beta on ovaries of female white rats (Rattus norvegicus). https://www.openveterinaryjournal.com/?mno=252103 [Access: September 01, 2025]. doi:10.5455/OVJ.2025.v15.i8.48

AMA (American Medical Association) Style

Hendrawan VF, Pritama A, Firmawati A, , Lisnanti EF. Effectiveness of papaya leaf extract (Carica papaya L.) as an infertility agent on estrus cycle length and expression of estrogen receptor beta on ovaries of female white rats (Rattus norvegicus). Open Vet. J.. 2025; 15(8): 3831-3837. doi:10.5455/OVJ.2025.v15.i8.48


Vancouver/ICMJE Style

Hendrawan VF, Pritama A, Firmawati A, , Lisnanti EF. Effectiveness of papaya leaf extract (Carica papaya L.) as an infertility agent on estrus cycle length and expression of estrogen receptor beta on ovaries of female white rats (Rattus norvegicus). Open Vet. J.. (2025), [cited September 01, 2025]; 15(8): 3831-3837. doi:10.5455/OVJ.2025.v15.i8.48


Harvard Style

Hendrawan, V. F., Pritama, . A., Firmawati, . A., & Lisnanti, . E. F. (2025) Effectiveness of papaya leaf extract (Carica papaya L.) as an infertility agent on estrus cycle length and expression of estrogen receptor beta on ovaries of female white rats (Rattus norvegicus). Open Vet. J., 15 (8), 3831-3837. doi:10.5455/OVJ.2025.v15.i8.48


Turabian Style

Hendrawan, Viski Fitri, Alsya Pritama, Aulia Firmawati, Intan Firdha Olien Noor Al Ichsan, and Ertika Fitri Lisnanti. 2025. Effectiveness of papaya leaf extract (Carica papaya L.) as an infertility agent on estrus cycle length and expression of estrogen receptor beta on ovaries of female white rats (Rattus norvegicus). Open Veterinary Journal, 15 (8), 3831-3837. doi:10.5455/OVJ.2025.v15.i8.48


Chicago Style

Hendrawan, Viski Fitri, Alsya Pritama, Aulia Firmawati, Intan Firdha Olien Noor Al Ichsan, and Ertika Fitri Lisnanti. "Effectiveness of papaya leaf extract (Carica papaya L.) as an infertility agent on estrus cycle length and expression of estrogen receptor beta on ovaries of female white rats (Rattus norvegicus)." Open Veterinary Journal 15 (2025), 3831-3837. doi:10.5455/OVJ.2025.v15.i8.48


MLA (The Modern Language Association) Style

Hendrawan, Viski Fitri, Alsya Pritama, Aulia Firmawati, Intan Firdha Olien Noor Al Ichsan, and Ertika Fitri Lisnanti. "Effectiveness of papaya leaf extract (Carica papaya L.) as an infertility agent on estrus cycle length and expression of estrogen receptor beta on ovaries of female white rats (Rattus norvegicus)." Open Veterinary Journal 15.8 (2025), 3831-3837. Print. doi:10.5455/OVJ.2025.v15.i8.48


APA (American Psychological Association) Style

Hendrawan, V. F., Pritama, . A., Firmawati, . A., & Lisnanti, . E. F. (2025) Effectiveness of papaya leaf extract (Carica papaya L.) as an infertility agent on estrus cycle length and expression of estrogen receptor beta on ovaries of female white rats (Rattus norvegicus). Open Veterinary Journal, 15 (8), 3831-3837. doi:10.5455/OVJ.2025.v15.i8.48


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