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Research Article


Kinematic and morphological evaluation of frozen Friesian Holstein semen stored for 33, 30, 27, and 24 years

Ahmad Budi Purnawan, Rimayanti Rimayanti, Suherni Susilowati, Erma Safitri, Tatik Hernawati, Imam Mustofa, Zulfi Nur Amrina Rosyada, Adeyinka Oye Akintunde, Aswin Rafif Khairullah.


Abstract
Background:
Fertility is determined by sperm motility, kinematics, and morphology. Motility is the most commonly used metric worldwide, aside from movement score and semen concentration, and is one of the semen quality indicators included in the Indonesian national standard (SNI 4869.1-2017) for frozen semen quality.

Aim:
This study aimed to determine the effect of frozen semen storage for 33, 30, 27, and 24 years on the sperm kinematics, and morphology of frozen-thawed semen of FH bull.

Methods:
Samples were collected from the frozen semen of FH bull with five straws for each storage period. Evaluation of frozen-thawed sperm motility, kinematics, and morphology was used computer-assisted sperm analysis (CASA) IVOS® II sperm analyzer. Each sample was measured in five fields, observation included motility (%), progressivity (%), concentration (million spermatozoa/mL), kinematics parameters consist of the amplitude of lateral head displacement (ALH, µm), curvilinear velocity (VCL, µm/s), straight-line velocity (VSL, µm/s), average path velocity (VAP, µm/s), linearity (LIN=VSL/VCL, %), wobble (WOB=VAP/VCL, %) and straightness (STR=VSL/VAP, %).

Results:
Based on statistical observation, the result of the research was not significantly different (p > 0.05) among storage years for sperm concentration and motility, kinematics, static, and sperm morphological of FH bull frozen-thawed semen stored for 24, 27, 30 and 33 years. Moreover, sperm motility was >40%, meeting Indonesian frozen bovine semen standards.

Conclusion:
It could be concluded that FH bull frozen-thawed semen of 33, 30, 27, and 24 years did not differ in sperm concentration, motility, kinematics, and sperm morphology and fulfilled the standards for artificial insemination.

Key words: FH bull, Food security, Frozen semen storage, Sperm kinematics, Sperm morphology


 
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How to Cite this Article
Pubmed Style

Purnawan AB, Rimayanti R, Susilowati S, Safitri E, Hernawati T, Mustofa I, Rosyada ZNA, Akintunde AO, Khairullah AR. Kinematic and morphological evaluation of frozen Friesian Holstein semen stored for 33, 30, 27, and 24 years. Open Vet J. 2025; 15(4): 1747-1756. doi:10.5455/OVJ.2025.v15.i4.26

Web Style

Purnawan AB, Rimayanti R, Susilowati S, Safitri E, Hernawati T, Mustofa I, Rosyada ZNA, Akintunde AO, Khairullah AR. Kinematic and morphological evaluation of frozen Friesian Holstein semen stored for 33, 30, 27, and 24 years. https://www.openveterinaryjournal.com/?mno=237765 [Access: May 07, 2025]. doi:10.5455/OVJ.2025.v15.i4.26

AMA (American Medical Association) Style

Purnawan AB, Rimayanti R, Susilowati S, Safitri E, Hernawati T, Mustofa I, Rosyada ZNA, Akintunde AO, Khairullah AR. Kinematic and morphological evaluation of frozen Friesian Holstein semen stored for 33, 30, 27, and 24 years. Open Vet J. 2025; 15(4): 1747-1756. doi:10.5455/OVJ.2025.v15.i4.26


Vancouver/ICMJE Style

Purnawan AB, Rimayanti R, Susilowati S, Safitri E, Hernawati T, Mustofa I, Rosyada ZNA, Akintunde AO, Khairullah AR. Kinematic and morphological evaluation of frozen Friesian Holstein semen stored for 33, 30, 27, and 24 years. Open Vet J. (2025), [cited May 07, 2025]; 15(4): 1747-1756. doi:10.5455/OVJ.2025.v15.i4.26


Harvard Style

Purnawan, A. B., Rimayanti, . R., Susilowati, . S., Safitri, . E., Hernawati, . T., Mustofa, . I., Rosyada, . Z. N. A., Akintunde, . A. O. & Khairullah, . A. R. (2025) Kinematic and morphological evaluation of frozen Friesian Holstein semen stored for 33, 30, 27, and 24 years. Open Vet J, 15 (4), 1747-1756. doi:10.5455/OVJ.2025.v15.i4.26


Turabian Style

Purnawan, Ahmad Budi, Rimayanti Rimayanti, Suherni Susilowati, Erma Safitri, Tatik Hernawati, Imam Mustofa, Zulfi Nur Amrina Rosyada, Adeyinka Oye Akintunde, and Aswin Rafif Khairullah. 2025. Kinematic and morphological evaluation of frozen Friesian Holstein semen stored for 33, 30, 27, and 24 years. Open Veterinary Journal, 15 (4), 1747-1756. doi:10.5455/OVJ.2025.v15.i4.26


Chicago Style

Purnawan, Ahmad Budi, Rimayanti Rimayanti, Suherni Susilowati, Erma Safitri, Tatik Hernawati, Imam Mustofa, Zulfi Nur Amrina Rosyada, Adeyinka Oye Akintunde, and Aswin Rafif Khairullah. "Kinematic and morphological evaluation of frozen Friesian Holstein semen stored for 33, 30, 27, and 24 years." Open Veterinary Journal 15 (2025), 1747-1756. doi:10.5455/OVJ.2025.v15.i4.26


MLA (The Modern Language Association) Style

Purnawan, Ahmad Budi, Rimayanti Rimayanti, Suherni Susilowati, Erma Safitri, Tatik Hernawati, Imam Mustofa, Zulfi Nur Amrina Rosyada, Adeyinka Oye Akintunde, and Aswin Rafif Khairullah. "Kinematic and morphological evaluation of frozen Friesian Holstein semen stored for 33, 30, 27, and 24 years." Open Veterinary Journal 15.4 (2025), 1747-1756. Print. doi:10.5455/OVJ.2025.v15.i4.26


APA (American Psychological Association) Style

Purnawan, A. B., Rimayanti, . R., Susilowati, . S., Safitri, . E., Hernawati, . T., Mustofa, . I., Rosyada, . Z. N. A., Akintunde, . A. O. & Khairullah, . A. R. (2025) Kinematic and morphological evaluation of frozen Friesian Holstein semen stored for 33, 30, 27, and 24 years. Open Veterinary Journal, 15 (4), 1747-1756. doi:10.5455/OVJ.2025.v15.i4.26


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