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Research Article


Immunohistochemical study of scrapie in naturally affected sheep in the east of Libya

Fawzia Mohamed, Ayiman Aboulqassim, Monier Sharif, Salh Belgasem, Abraheem Omar, Nagi Saeed.


Abstract
Background:
The most common natural prion disease that primarily affects sheep and goats is scrapie. It belongs to a group of disorders known as transmissible spongiform encephalopathies (TSEs), which impact both humans and animals.
Aim:
The research is aimed to examine and confirm the presence of Scrapie in Libya using
immunohistochemistry (IHC) techniques.
Methods:
Brain samples were collected from thirty-three sheep older than two years of age showing clinical signs resembling to scrapie during the period between 2018 to 2023, regardless of race or gender. Three animals, six months old, healthy, and without any symptoms, were used as negative controls. Different parts of the brain, including obex and cerebellum, were taken from each case. The IHC technique used in this study involved staining with monoclonal antibody L42 and DAB (3,3′-diaminobenzidine) as a chromogenic substrate.
Results:
The IHC examination showed the expression of prion proteins in brain tissue in twenty-three samples. The staining intensity was markedly observed in the neuronal cell bodies and around blood vessels.
Conclusion:
The findings of this study provide evidence that Scrapie exists in Libya.

Key words: Immunohistochemistry, Libya, Prion, Scrapie, Sheep


 
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How to Cite this Article
Pubmed Style

Mohamed F, Aboulqassim A, Sharif M, Belgasem S, Omar A, Saeed N. Immunohistochemical study of scrapie in naturally affected sheep in the east of Libya. Open Vet J. 2024; 14(8): 1843-1849. doi:10.5455/OVJ.2024.v14.i8.12

Web Style

Mohamed F, Aboulqassim A, Sharif M, Belgasem S, Omar A, Saeed N. Immunohistochemical study of scrapie in naturally affected sheep in the east of Libya. https://www.openveterinaryjournal.com/?mno=197391 [Access: October 03, 2024]. doi:10.5455/OVJ.2024.v14.i8.12

AMA (American Medical Association) Style

Mohamed F, Aboulqassim A, Sharif M, Belgasem S, Omar A, Saeed N. Immunohistochemical study of scrapie in naturally affected sheep in the east of Libya. Open Vet J. 2024; 14(8): 1843-1849. doi:10.5455/OVJ.2024.v14.i8.12


Vancouver/ICMJE Style

Mohamed F, Aboulqassim A, Sharif M, Belgasem S, Omar A, Saeed N. Immunohistochemical study of scrapie in naturally affected sheep in the east of Libya. Open Vet J. (2024), [cited October 03, 2024]; 14(8): 1843-1849. doi:10.5455/OVJ.2024.v14.i8.12


Harvard Style

Mohamed, F., Aboulqassim, . A., Sharif, . M., Belgasem, . S., Omar, . A. & Saeed, . N. (2024) Immunohistochemical study of scrapie in naturally affected sheep in the east of Libya. Open Vet J, 14 (8), 1843-1849. doi:10.5455/OVJ.2024.v14.i8.12


Turabian Style

Mohamed, Fawzia, Ayiman Aboulqassim, Monier Sharif, Salh Belgasem, Abraheem Omar, and Nagi Saeed. 2024. Immunohistochemical study of scrapie in naturally affected sheep in the east of Libya. Open Veterinary Journal, 14 (8), 1843-1849. doi:10.5455/OVJ.2024.v14.i8.12


Chicago Style

Mohamed, Fawzia, Ayiman Aboulqassim, Monier Sharif, Salh Belgasem, Abraheem Omar, and Nagi Saeed. "Immunohistochemical study of scrapie in naturally affected sheep in the east of Libya." Open Veterinary Journal 14 (2024), 1843-1849. doi:10.5455/OVJ.2024.v14.i8.12


MLA (The Modern Language Association) Style

Mohamed, Fawzia, Ayiman Aboulqassim, Monier Sharif, Salh Belgasem, Abraheem Omar, and Nagi Saeed. "Immunohistochemical study of scrapie in naturally affected sheep in the east of Libya." Open Veterinary Journal 14.8 (2024), 1843-1849. Print. doi:10.5455/OVJ.2024.v14.i8.12


APA (American Psychological Association) Style

Mohamed, F., Aboulqassim, . A., Sharif, . M., Belgasem, . S., Omar, . A. & Saeed, . N. (2024) Immunohistochemical study of scrapie in naturally affected sheep in the east of Libya. Open Veterinary Journal, 14 (8), 1843-1849. doi:10.5455/OVJ.2024.v14.i8.12


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