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Research Article


Epidemiological investigation of Mycobacterium bovis infection by PCR in dairy cattle milk in Pangalengan, Bandung Regency, West Java, Indonesia

Roostita L. Balia, Kuswandewi Mutyara, Ardini Saptaningsih Raksanagara, Tyagita Hartady, Sarasati Windria, Shafia Khairani, Suseno Amien, Nilla Krisna Sari, Muhammad Farid Rizal.


Abstract
Background:
Bovine tuberculosis (bTB), caused by Mycobacterium bovis, is a zoonotic disease that primarily affects cattle and may be transmitted to humans through aerosol exposure or consumption of unpasteurized milk. Although Indonesia has no officially reported clinical cases of bTB, molecular surveillance remains essential in dairy production centers.

Aim:
This study aimed to detect M. bovis in dairy cattle milk using polymerase chain reaction (PCR) and to assess the potential risk of zoonotic transmission in Pangalengan, West Java.

Methods:
A total of 60 milk samples were collected from lactating dairy cows in three villages (Pangalengan, Sukamanah, and Margamulya). Deoxyribonucleic acid extraction was performed using a commercial kit, followed by conventional PCR targeting CSB1 and CSB2 gene regions specific for M. bovis. PCR products were analysed by agarose gel electrophoresis.

Results:
All 60 milk samples (100%) tested negative for M. bovis. No specific 168 bp amplicon was detected in any sample; the positive control showed clear amplification.

Conclusion:
No evidence of M. bovis infection was detected in dairy milk samples from Pangalengan. These findings suggest a minimal current risk of zoonotic tuberculosis transmission via milk in this region; however, continued surveillance is recommended.

Key words: Bovine tuberculosis; Dairy cattle; Milk; PCR; Zoonosis.


 
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How to Cite this Article
Pubmed Style

Balia RL, Mutyara K, Raksanagara AS, Hartady T, Windria S, Khairani S, Amien S, Sari NK, Rizal MF. Epidemiological investigation of Mycobacterium bovis infection by PCR in dairy cattle milk in Pangalengan, Bandung Regency, West Java, Indonesia. Open Vet. J.. 2026; 16(5): 2757-2765. doi:10.5455/OVJ.2026.v16.i5.18

Web Style

Balia RL, Mutyara K, Raksanagara AS, Hartady T, Windria S, Khairani S, Amien S, Sari NK, Rizal MF. Epidemiological investigation of Mycobacterium bovis infection by PCR in dairy cattle milk in Pangalengan, Bandung Regency, West Java, Indonesia. https://www.openveterinaryjournal.com/?mno=303395 [Access: June 10, 2026]. doi:10.5455/OVJ.2026.v16.i5.18

AMA (American Medical Association) Style

Balia RL, Mutyara K, Raksanagara AS, Hartady T, Windria S, Khairani S, Amien S, Sari NK, Rizal MF. Epidemiological investigation of Mycobacterium bovis infection by PCR in dairy cattle milk in Pangalengan, Bandung Regency, West Java, Indonesia. Open Vet. J.. 2026; 16(5): 2757-2765. doi:10.5455/OVJ.2026.v16.i5.18


Vancouver/ICMJE Style

Balia RL, Mutyara K, Raksanagara AS, Hartady T, Windria S, Khairani S, Amien S, Sari NK, Rizal MF. Epidemiological investigation of Mycobacterium bovis infection by PCR in dairy cattle milk in Pangalengan, Bandung Regency, West Java, Indonesia. Open Vet. J.. (2026), [cited June 10, 2026]; 16(5): 2757-2765. doi:10.5455/OVJ.2026.v16.i5.18


Harvard Style

Balia, R. L., Mutyara, . K., Raksanagara, . A. S., Hartady, . T., Windria, . S., Khairani, . S., Amien, . S., Sari, . N. K. & Rizal, . M. F. (2026) Epidemiological investigation of Mycobacterium bovis infection by PCR in dairy cattle milk in Pangalengan, Bandung Regency, West Java, Indonesia. Open Vet. J., 16 (5), 2757-2765. doi:10.5455/OVJ.2026.v16.i5.18


Turabian Style

Balia, Roostita L., Kuswandewi Mutyara, Ardini Saptaningsih Raksanagara, Tyagita Hartady, Sarasati Windria, Shafia Khairani, Suseno Amien, Nilla Krisna Sari, and Muhammad Farid Rizal. 2026. Epidemiological investigation of Mycobacterium bovis infection by PCR in dairy cattle milk in Pangalengan, Bandung Regency, West Java, Indonesia. Open Veterinary Journal, 16 (5), 2757-2765. doi:10.5455/OVJ.2026.v16.i5.18


Chicago Style

Balia, Roostita L., Kuswandewi Mutyara, Ardini Saptaningsih Raksanagara, Tyagita Hartady, Sarasati Windria, Shafia Khairani, Suseno Amien, Nilla Krisna Sari, and Muhammad Farid Rizal. "Epidemiological investigation of Mycobacterium bovis infection by PCR in dairy cattle milk in Pangalengan, Bandung Regency, West Java, Indonesia." Open Veterinary Journal 16 (2026), 2757-2765. doi:10.5455/OVJ.2026.v16.i5.18


MLA (The Modern Language Association) Style

Balia, Roostita L., Kuswandewi Mutyara, Ardini Saptaningsih Raksanagara, Tyagita Hartady, Sarasati Windria, Shafia Khairani, Suseno Amien, Nilla Krisna Sari, and Muhammad Farid Rizal. "Epidemiological investigation of Mycobacterium bovis infection by PCR in dairy cattle milk in Pangalengan, Bandung Regency, West Java, Indonesia." Open Veterinary Journal 16.5 (2026), 2757-2765. Print. doi:10.5455/OVJ.2026.v16.i5.18


APA (American Psychological Association) Style

Balia, R. L., Mutyara, . K., Raksanagara, . A. S., Hartady, . T., Windria, . S., Khairani, . S., Amien, . S., Sari, . N. K. & Rizal, . M. F. (2026) Epidemiological investigation of Mycobacterium bovis infection by PCR in dairy cattle milk in Pangalengan, Bandung Regency, West Java, Indonesia. Open Veterinary Journal, 16 (5), 2757-2765. doi:10.5455/OVJ.2026.v16.i5.18


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