Prevalence, risk factors, and molecular identification of Eimeria spp. in small ruminants from three districts in Java, Indonesia

Estha Gusmalia Kustika; Mila Putri Anggraini; Fathur Rohman Haryadi; Fitrine Ekawasti; Madarina Wasissa; Fajar Budi Lestari

doi:10.5455/OVJ.2026.v16.i7.47

2026, Vol: 16, Issue: 7

16 / 7Current Issue Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Required files to be uploaded

OVJ Manuscript Submissio Form
Title Page
mainarticle_template

« Previous Article

Research Article

Open Vet. J.. 2026; 16(7): 4670-4679

doi: 10.5455/OVJ.2026.v16.i7.47

Prevalence, risk factors, and molecular identification of Eimeria spp. in small ruminants from three districts in Java, Indonesia

Estha Gusmalia Kustika, Mila Putri Anggraini, Fathur Rohman Haryadi, Fitrine Ekawasti, Madarina Wasissa, Fajar Budi Lestari.

Abstract
Background:
Coccidiosis caused by protozoa of the genus Eimeria remains a leading parasitic constraint in small ruminants production systems worldwide.

Aim:
Determining the prevalence, risk factors, and species of Eimeria spp. that infect goats and sheep.

Methods:
Fresh fecal samples were collected from 112 goats and sheep across Sragen, Sleman, and Ngawi regencies. The samples were examined using qualitative (direct smear and flotation) and quantitative (McMaster) methods. For molecular analysis, genomic DNA was extracted from samples with >200 oocysts per gram (OPG). The 18S rRNA gene was targeted via PCR, followed by sequencing of representative amplicons to identify the species. Data were analyzed using descriptive statistics and logistic regression.

Results:
The study found a high prevalence of 90.18% (101/112). Molecular characterization identified the presence of Eimeria crandallis and Eimeria christenseni. The results confirmed that sex, age, and type of forage were not associated risk factors for Eimeria spp. infection (P > 0.05).

Conclusion:
The prevalence of Eimeria infection in sheep and goats was high although no clinical symptoms were observed. E. crandallis and E. christenseni were identified. Multivariate analysis showed that sex, age, and type of forage insignificantly affected the occurrence of Eimeria infection. Prevention strategies are needed to minimize economic losses in small ruminants production.

Key words: Eimeria spp.; Fecal samples; Goats; Java; Sheep.

	ARTICLE TOOLS
	Abstract
	PDF Fulltext
	How to cite this article
	Citation Tools
	Related Records
	Articles by Estha Gusmalia Kustika Articles by Mila Putri Anggraini Articles by Fathur Rohman Haryadi Articles by Fitrine Ekawasti Articles by Madarina Wasissa Articles by Fajar Budi Lestari
	on Google
	on Google Scholar

How to Cite this Article

Pubmed Style

Kustika EG, Anggraini MP, Haryadi FR, Ekawasti F, Wasissa M, Lestari FB. Prevalence, risk factors, and molecular identification of Eimeria spp. in small ruminants from three districts in Java, Indonesia. doi:10.5455/OVJ.2026.v16.i7.47

Web Style

Kustika EG, Anggraini MP, Haryadi FR, Ekawasti F, Wasissa M, Lestari FB. Prevalence, risk factors, and molecular identification of Eimeria spp. in small ruminants from three districts in Java, Indonesia. https://www.openveterinaryjournal.com/?mno=299931 [Access: July 15, 2026]. doi:10.5455/OVJ.2026.v16.i7.47

AMA (American Medical Association) Style

Kustika EG, Anggraini MP, Haryadi FR, Ekawasti F, Wasissa M, Lestari FB. Prevalence, risk factors, and molecular identification of Eimeria spp. in small ruminants from three districts in Java, Indonesia. doi:10.5455/OVJ.2026.v16.i7.47

Vancouver/ICMJE Style

Harvard Style

Kustika, E. G., Anggraini, . M. P., Haryadi, . F. R., Ekawasti, . F., Wasissa, . M. & Lestari, . F. B. (2026) Prevalence, risk factors, and molecular identification of Eimeria spp. in small ruminants from three districts in Java, Indonesia. doi:10.5455/OVJ.2026.v16.i7.47

Turabian Style

Kustika, Estha Gusmalia, Mila Putri Anggraini, Fathur Rohman Haryadi, Fitrine Ekawasti, Madarina Wasissa, and Fajar Budi Lestari. 2026. Prevalence, risk factors, and molecular identification of Eimeria spp. in small ruminants from three districts in Java, Indonesia. doi:10.5455/OVJ.2026.v16.i7.47

Chicago Style

Kustika, Estha Gusmalia, Mila Putri Anggraini, Fathur Rohman Haryadi, Fitrine Ekawasti, Madarina Wasissa, and Fajar Budi Lestari. "Prevalence, risk factors, and molecular identification of Eimeria spp. in small ruminants from three districts in Java, Indonesia." doi:10.5455/OVJ.2026.v16.i7.47

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

Author Login Reviewer Login About Publisher Open Access Policy Editorial Policies Editorial Review Policy Editorial & Peer Review Process Publication Ethics and Publication Malpractice Statement Conflict of Interest Policy Plagiarism Policy Protection of Research Participants (Statement On Human And Animal Rights)Corrections, Retractions & Expressions of Concern Self-Archiving Policies Digital Archiving & Preservation Policies Statement of Informed Consent Advertising Policy Terms of Use License Information Copyright Information

About Open Veterinary Journal

Open Veterinary Journal provides high quality peer review process for original research articles, review articles, short communications and case reports i ... Read more.

For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes

Editor-in-Chief

Open Veterinary Journal

Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

Email: ibrahim.eldaghayes@vetmed.edu.ly

About Open Veterinary Journal

Contact Information

How to cite this article