E-ISSN 2218-6050 | ISSN 2226-4485
 
2025, Vol: 15, Issue: 11
15 / 11Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles
Required files to be uploaded

OVJ Manuscript Submissio Form
mainarticle_template

Research Article


Molecular identification of Brucella abortus in secondary hosts of goats and sheep in Magelang, Central Java, Indonesia

David Ardiyanto, Hastari Wuryastuty, Yanuartono Yanuartono, Sarmin Sarmin, Raden Wasito, Claude Mona Airin, Diah Tri Widiati, Wida Wahidah Mubarokah.


Abstract
Background:
Brucella abortus is primarily associated with cattle; however, its detection in small ruminants has been increasingly reported, raising concerns about interspecies transmission and public health risks.

Aim:
This study aimed to detect and genetically characterize B. abortus in goats and sheep using molecular techniques, assess genetic variation within the IS711 gene, and evaluate phylogenetic relationships among isolates.

Methods:
Blood samples were collected from 102 small ruminants (32 goats and 70 sheep) with a history of reproductive disorders in Magelang District, Central Java, between October and December 2024. DNA was extracted and analyzed using Abortus, Melitensis, Ovis, Suis Polymerase Chain Reaction (AMOS)-PCR targeting the IS711 gene. Positive amplicons were sequenced and subjected to BLAST analysis, phylogenetic reconstruction (MEGA 12), and genetic distance estimation using the Kimura-2 parameter.

Results:
AMOS-PCR was used to detect B. abortus Deoxyribonucleic acid in 40 animals (39.2%). Sequencing showed >99% identity with reference B. abortus strains. Phylogenetic analysis revealed low genetic divergence (0.000–0.004), indicating a common infection source. Notably, several isolates exhibited limited sequence variation, including a double guanine insertion at positions 8–9 and a C→T transition at position 40 of IS711.

Conclusion:
This study demonstrates that B. abortus can infect small ruminants through interspecies transmission under mixed grazing systems. The findings emphasize the need to expand surveillance and control efforts beyond cattle to include sheep and goats, strengthening brucellosis management within a One Health framework.

Key words: Brucella abortus; Goats; Sheep; AMOS PCR; Phylogenetic IS711 gene.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by David Ardiyanto
Articles by Hastari Wuryastuty
Articles by Yanuartono Yanuartono
Articles by Sarmin Sarmin
Articles by Raden Wasito
Articles by Claude Mona Airin
Articles by Diah Tri Widiati
Articles by Wida Wahidah Mubarokah
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Ardiyanto D, Wuryastuty H, Yanuartono Y, Sarmin S, Wasito R, Airin CM, Widiati DT, Mubarokah WW. Molecular identification of Brucella abortus in secondary hosts of goats and sheep in Magelang, Central Java, Indonesia. Open Vet. J.. 2025; 15(11): 5658-5668. doi:10.5455/OVJ.2025.v15.i11.22

Web Style

Ardiyanto D, Wuryastuty H, Yanuartono Y, Sarmin S, Wasito R, Airin CM, Widiati DT, Mubarokah WW. Molecular identification of Brucella abortus in secondary hosts of goats and sheep in Magelang, Central Java, Indonesia. https://www.openveterinaryjournal.com/?mno=268811 [Access: November 30, 2025]. doi:10.5455/OVJ.2025.v15.i11.22

AMA (American Medical Association) Style

Ardiyanto D, Wuryastuty H, Yanuartono Y, Sarmin S, Wasito R, Airin CM, Widiati DT, Mubarokah WW. Molecular identification of Brucella abortus in secondary hosts of goats and sheep in Magelang, Central Java, Indonesia. Open Vet. J.. 2025; 15(11): 5658-5668. doi:10.5455/OVJ.2025.v15.i11.22


Vancouver/ICMJE Style

Ardiyanto D, Wuryastuty H, Yanuartono Y, Sarmin S, Wasito R, Airin CM, Widiati DT, Mubarokah WW. Molecular identification of Brucella abortus in secondary hosts of goats and sheep in Magelang, Central Java, Indonesia. Open Vet. J.. (2025), [cited November 30, 2025]; 15(11): 5658-5668. doi:10.5455/OVJ.2025.v15.i11.22


Harvard Style

Ardiyanto, D., Wuryastuty, . H., Yanuartono, . Y., Sarmin, . S., Wasito, . R., Airin, . C. M., Widiati, . D. T. & Mubarokah, . W. W. (2025) Molecular identification of Brucella abortus in secondary hosts of goats and sheep in Magelang, Central Java, Indonesia. Open Vet. J., 15 (11), 5658-5668. doi:10.5455/OVJ.2025.v15.i11.22


Turabian Style

Ardiyanto, David, Hastari Wuryastuty, Yanuartono Yanuartono, Sarmin Sarmin, Raden Wasito, Claude Mona Airin, Diah Tri Widiati, and Wida Wahidah Mubarokah. 2025. Molecular identification of Brucella abortus in secondary hosts of goats and sheep in Magelang, Central Java, Indonesia. Open Veterinary Journal, 15 (11), 5658-5668. doi:10.5455/OVJ.2025.v15.i11.22


Chicago Style

Ardiyanto, David, Hastari Wuryastuty, Yanuartono Yanuartono, Sarmin Sarmin, Raden Wasito, Claude Mona Airin, Diah Tri Widiati, and Wida Wahidah Mubarokah. "Molecular identification of Brucella abortus in secondary hosts of goats and sheep in Magelang, Central Java, Indonesia." Open Veterinary Journal 15 (2025), 5658-5668. doi:10.5455/OVJ.2025.v15.i11.22


MLA (The Modern Language Association) Style

Ardiyanto, David, Hastari Wuryastuty, Yanuartono Yanuartono, Sarmin Sarmin, Raden Wasito, Claude Mona Airin, Diah Tri Widiati, and Wida Wahidah Mubarokah. "Molecular identification of Brucella abortus in secondary hosts of goats and sheep in Magelang, Central Java, Indonesia." Open Veterinary Journal 15.11 (2025), 5658-5668. Print. doi:10.5455/OVJ.2025.v15.i11.22


APA (American Psychological Association) Style

Ardiyanto, D., Wuryastuty, . H., Yanuartono, . Y., Sarmin, . S., Wasito, . R., Airin, . C. M., Widiati, . D. T. & Mubarokah, . W. W. (2025) Molecular identification of Brucella abortus in secondary hosts of goats and sheep in Magelang, Central Java, Indonesia. Open Veterinary Journal, 15 (11), 5658-5668. doi:10.5455/OVJ.2025.v15.i11.22


Author LoginReviewer LoginAbout PublisherPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyTerms of UseLicense InformationCopyright Information

About Open Veterinary Journal

Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Owner, Founder and Editor-in-Chief

Open Veterinary Journal


Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

and


Eldaghayes Publisher

www.eldaghayes.com 

Email: ibrahim.eldaghayes@vetmed.edu.ly