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Research Article


Pasteurella multocida genome assembly of Indonesian local isolates using long-read Oxford Nanopore Technology sequencing

Sri Suryatmiati Prihandani, I. Wayan Teguh Wibawan, Susan Maphilindawati Noor, Safika Safika, Aswin Rafif Khairullah, Susanti Susanti, Sutiastuti Wahyuwardani, Andriani Andriani, Sumarningsih Sumarningsih, Heri Kurnianto, Tati Ariyanti, Yudi Adinata, Harimurti Nuradji, Alif Rahman Rohim Puarada.


Abstract
Background:
Pasteurella multocida is a Gram-negative bacterium responsible for various diseases in animals, including Haemorrhagic Septicaemia (HS). Oxford Nanopore Technologies’ platform streams sequencing data immediately, allowing for on-the-fly analysis. This capability enables researchers to monitor experiments in real-time and make prompt decisions, which is particularly beneficial in clinical diagnostics and outbreak surveillance.

Aim:
This study presents the genome assembly of two P. multocida isolates using long-read sequencing technology provided by Oxford Nanopore Technologies.

Methods:
This method involves passing DNA strands through nanopores and detecting changes in electrical current to determine the nucleotide sequence. The long-read capability of ONT's technology allows for sequencing of extensive DNA fragments, which is particularly beneficial for resolving complex genomic regions and achieving high-contiguity genome assemblies.

Results:
The study achieved high-quality, single-contig genomes with sizes of 2.35 Mbp and 2.29 Mbp, and completeness scores exceeding 99% (BUSCO). These assemblies provide valuable resources for functional analyses, enhancing the understanding of virulence factors and the pathogenesis of Haemorrhagic Septicaemia in Indonesian livestock.

Conclusion:
The study successfully assembled the genomes of two P. multocida isolates from Lampung (2952) and Kupang, Nusa Tenggara Timur (NTT), Indonesia, using Oxford Nanopore Technologies' long-read sequencing. The resulting single-contig genomes, measuring 2.35 Mbp and 2.29 Mbp respectively, exhibited high completeness scores (BUSCO: >99%). These high-quality assemblies enhance genomic contiguity, providing valuable resources for functional analyses aimed at understanding the virulence factors and pathogenesis of Haemorrhagic Septicaemia in Indonesian livestock.

Key words: Genome assembly, Hemorrhagic septicemia, Oxford Nanopore Technologies, P. multocida, Whole genome sequencing


 
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How to Cite this Article
Pubmed Style

Prihandani SS, Wibawan IWT, Noor SM, Safika S, Khairullah AR, Susanti S, Wahyuwardani S, Andriani A, Sumarningsih S, Kurnianto H, Ariyanti T, Adinata Y, Nuradji H, Puarada ARR. Pasteurella multocida genome assembly of Indonesian local isolates using long-read Oxford Nanopore Technology sequencing. Open Vet J. 2025; 15(6): 2782-2788. doi:10.5455/OVJ.2025.v15.i6.46

Web Style

Prihandani SS, Wibawan IWT, Noor SM, Safika S, Khairullah AR, Susanti S, Wahyuwardani S, Andriani A, Sumarningsih S, Kurnianto H, Ariyanti T, Adinata Y, Nuradji H, Puarada ARR. Pasteurella multocida genome assembly of Indonesian local isolates using long-read Oxford Nanopore Technology sequencing. https://www.openveterinaryjournal.com/?mno=250015 [Access: July 12, 2025]. doi:10.5455/OVJ.2025.v15.i6.46

AMA (American Medical Association) Style

Prihandani SS, Wibawan IWT, Noor SM, Safika S, Khairullah AR, Susanti S, Wahyuwardani S, Andriani A, Sumarningsih S, Kurnianto H, Ariyanti T, Adinata Y, Nuradji H, Puarada ARR. Pasteurella multocida genome assembly of Indonesian local isolates using long-read Oxford Nanopore Technology sequencing. Open Vet J. 2025; 15(6): 2782-2788. doi:10.5455/OVJ.2025.v15.i6.46


Vancouver/ICMJE Style

Prihandani SS, Wibawan IWT, Noor SM, Safika S, Khairullah AR, Susanti S, Wahyuwardani S, Andriani A, Sumarningsih S, Kurnianto H, Ariyanti T, Adinata Y, Nuradji H, Puarada ARR. Pasteurella multocida genome assembly of Indonesian local isolates using long-read Oxford Nanopore Technology sequencing. Open Vet J. (2025), [cited July 12, 2025]; 15(6): 2782-2788. doi:10.5455/OVJ.2025.v15.i6.46


Harvard Style

Prihandani, S. S., Wibawan, . I. W. T., Noor, . S. M., Safika, . S., Khairullah, . A. R., Susanti, . S., Wahyuwardani, . S., Andriani, . A., Sumarningsih, . S., Kurnianto, . H., Ariyanti, . T., Adinata, . Y., Nuradji, . H. & Puarada, . A. R. R. (2025) Pasteurella multocida genome assembly of Indonesian local isolates using long-read Oxford Nanopore Technology sequencing. Open Vet J, 15 (6), 2782-2788. doi:10.5455/OVJ.2025.v15.i6.46


Turabian Style

Prihandani, Sri Suryatmiati, I. Wayan Teguh Wibawan, Susan Maphilindawati Noor, Safika Safika, Aswin Rafif Khairullah, Susanti Susanti, Sutiastuti Wahyuwardani, Andriani Andriani, Sumarningsih Sumarningsih, Heri Kurnianto, Tati Ariyanti, Yudi Adinata, Harimurti Nuradji, and Alif Rahman Rohim Puarada. 2025. Pasteurella multocida genome assembly of Indonesian local isolates using long-read Oxford Nanopore Technology sequencing. Open Veterinary Journal, 15 (6), 2782-2788. doi:10.5455/OVJ.2025.v15.i6.46


Chicago Style

Prihandani, Sri Suryatmiati, I. Wayan Teguh Wibawan, Susan Maphilindawati Noor, Safika Safika, Aswin Rafif Khairullah, Susanti Susanti, Sutiastuti Wahyuwardani, Andriani Andriani, Sumarningsih Sumarningsih, Heri Kurnianto, Tati Ariyanti, Yudi Adinata, Harimurti Nuradji, and Alif Rahman Rohim Puarada. "Pasteurella multocida genome assembly of Indonesian local isolates using long-read Oxford Nanopore Technology sequencing." Open Veterinary Journal 15 (2025), 2782-2788. doi:10.5455/OVJ.2025.v15.i6.46


MLA (The Modern Language Association) Style

Prihandani, Sri Suryatmiati, I. Wayan Teguh Wibawan, Susan Maphilindawati Noor, Safika Safika, Aswin Rafif Khairullah, Susanti Susanti, Sutiastuti Wahyuwardani, Andriani Andriani, Sumarningsih Sumarningsih, Heri Kurnianto, Tati Ariyanti, Yudi Adinata, Harimurti Nuradji, and Alif Rahman Rohim Puarada. "Pasteurella multocida genome assembly of Indonesian local isolates using long-read Oxford Nanopore Technology sequencing." Open Veterinary Journal 15.6 (2025), 2782-2788. Print. doi:10.5455/OVJ.2025.v15.i6.46


APA (American Psychological Association) Style

Prihandani, S. S., Wibawan, . I. W. T., Noor, . S. M., Safika, . S., Khairullah, . A. R., Susanti, . S., Wahyuwardani, . S., Andriani, . A., Sumarningsih, . S., Kurnianto, . H., Ariyanti, . T., Adinata, . Y., Nuradji, . H. & Puarada, . A. R. R. (2025) Pasteurella multocida genome assembly of Indonesian local isolates using long-read Oxford Nanopore Technology sequencing. Open Veterinary Journal, 15 (6), 2782-2788. doi:10.5455/OVJ.2025.v15.i6.46


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