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Investigation of cisplatin-induced acute kidney injury using LC-HRMS and network pharmacology approaches in mixed Curcuma longa and Curcuma zedoaria extracts

Putri Reno Intan, Sukmayati Alegantina, Ani Isnawati, Lucie Widowati, Dian Sundari, Frans Dany, Agus Saputra, Sela Septima Mariya, Lina Noviyanti Sutardi, Agus Setiyono, Ekowati Handharyani.


Abstract
Background:
Cisplatin-induced acute kidney injury (AKI) is a serious complication of cancer therapy. Natural compounds, such as those found in Curcuma species, may exert protective effects through anti-inflammatory and antioxidant mechanisms.

Aim:
This study investigated the therapeutic mechanisms of combined Curcuma longa and Curcuma zedoaria extracts against cisplatin-induced kidney damage using UHPLC-HRMS and network pharmacology.

Methods:
An ethanolic extract was prepared and analyzed using UHPLC-Q-Orbitrap HRMS to identify active compounds. The antioxidant capacity was assessed using the DPPH assay. Network pharmacology was used to predict compound-target interactions and identify key proteins involved in AKI pathways.

Results:
UHPLC-Q-Orbitrap HRMS analysis identified 40 bioactive compounds, among which 22 met the OB >30% threshold. Network analysis revealed 14 overlapping protein targets associated with AKI from 485 compound-related targets and 198 disease-related targets.

Conclusion:
This study demonstrated that key phytochemicals in C. longa and C. zedoaria may act on multiple protein targets implicated in AKI, highlighting their potential as multi-target therapeutics for kidney protection and future drug development.

Key words: Curcuma longa, Kidney damage, UHPLC-Q-Orbitrap HRMS, Network pharmacology, Zedoaria


 
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How to Cite this Article
Pubmed Style

Intan PR, Alegantina S, Isnawati A, Widowati L, Sundari D, Dany F, Saputra A, Mariya SS, Sutardi LN, Setiyono A, Handharyani E. Investigation of cisplatin-induced acute kidney injury using LC-HRMS and network pharmacology approaches in mixed Curcuma longa and Curcuma zedoaria extracts. Open Vet. J.. 2025; 15(7): 3341-3351. doi:10.5455/OVJ.2025.v15.i7.46

Web Style

Intan PR, Alegantina S, Isnawati A, Widowati L, Sundari D, Dany F, Saputra A, Mariya SS, Sutardi LN, Setiyono A, Handharyani E. Investigation of cisplatin-induced acute kidney injury using LC-HRMS and network pharmacology approaches in mixed Curcuma longa and Curcuma zedoaria extracts. https://www.openveterinaryjournal.com/?mno=246232 [Access: August 22, 2025]. doi:10.5455/OVJ.2025.v15.i7.46

AMA (American Medical Association) Style

Intan PR, Alegantina S, Isnawati A, Widowati L, Sundari D, Dany F, Saputra A, Mariya SS, Sutardi LN, Setiyono A, Handharyani E. Investigation of cisplatin-induced acute kidney injury using LC-HRMS and network pharmacology approaches in mixed Curcuma longa and Curcuma zedoaria extracts. Open Vet. J.. 2025; 15(7): 3341-3351. doi:10.5455/OVJ.2025.v15.i7.46


Vancouver/ICMJE Style

Intan PR, Alegantina S, Isnawati A, Widowati L, Sundari D, Dany F, Saputra A, Mariya SS, Sutardi LN, Setiyono A, Handharyani E. Investigation of cisplatin-induced acute kidney injury using LC-HRMS and network pharmacology approaches in mixed Curcuma longa and Curcuma zedoaria extracts. Open Vet. J.. (2025), [cited August 22, 2025]; 15(7): 3341-3351. doi:10.5455/OVJ.2025.v15.i7.46


Harvard Style

Intan, P. R., Alegantina, . S., Isnawati, . A., Widowati, . L., Sundari, . D., Dany, . F., Saputra, . A., Mariya, . S. S., Sutardi, . L. N., Setiyono, . A. & Handharyani, . E. (2025) Investigation of cisplatin-induced acute kidney injury using LC-HRMS and network pharmacology approaches in mixed Curcuma longa and Curcuma zedoaria extracts. Open Vet. J., 15 (7), 3341-3351. doi:10.5455/OVJ.2025.v15.i7.46


Turabian Style

Intan, Putri Reno, Sukmayati Alegantina, Ani Isnawati, Lucie Widowati, Dian Sundari, Frans Dany, Agus Saputra, Sela Septima Mariya, Lina Noviyanti Sutardi, Agus Setiyono, and Ekowati Handharyani. 2025. Investigation of cisplatin-induced acute kidney injury using LC-HRMS and network pharmacology approaches in mixed Curcuma longa and Curcuma zedoaria extracts. Open Veterinary Journal, 15 (7), 3341-3351. doi:10.5455/OVJ.2025.v15.i7.46


Chicago Style

Intan, Putri Reno, Sukmayati Alegantina, Ani Isnawati, Lucie Widowati, Dian Sundari, Frans Dany, Agus Saputra, Sela Septima Mariya, Lina Noviyanti Sutardi, Agus Setiyono, and Ekowati Handharyani. "Investigation of cisplatin-induced acute kidney injury using LC-HRMS and network pharmacology approaches in mixed Curcuma longa and Curcuma zedoaria extracts." Open Veterinary Journal 15 (2025), 3341-3351. doi:10.5455/OVJ.2025.v15.i7.46


MLA (The Modern Language Association) Style

Intan, Putri Reno, Sukmayati Alegantina, Ani Isnawati, Lucie Widowati, Dian Sundari, Frans Dany, Agus Saputra, Sela Septima Mariya, Lina Noviyanti Sutardi, Agus Setiyono, and Ekowati Handharyani. "Investigation of cisplatin-induced acute kidney injury using LC-HRMS and network pharmacology approaches in mixed Curcuma longa and Curcuma zedoaria extracts." Open Veterinary Journal 15.7 (2025), 3341-3351. Print. doi:10.5455/OVJ.2025.v15.i7.46


APA (American Psychological Association) Style

Intan, P. R., Alegantina, . S., Isnawati, . A., Widowati, . L., Sundari, . D., Dany, . F., Saputra, . A., Mariya, . S. S., Sutardi, . L. N., Setiyono, . A. & Handharyani, . E. (2025) Investigation of cisplatin-induced acute kidney injury using LC-HRMS and network pharmacology approaches in mixed Curcuma longa and Curcuma zedoaria extracts. Open Veterinary Journal, 15 (7), 3341-3351. doi:10.5455/OVJ.2025.v15.i7.46


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