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Research Article


Assessment of the environmental transmission dynamics of Escherichia coli-producing ESBL in wet market broiler chickens: A high-burden food safety concern

Kuntaman Kuntaman, Masfufatun Masfufatun, Freshinta Jellia Wibisono, Rosantia Sarassari, Wahyu Setyorini, Putu Oky Ari Tania, Toshiro Shirakawa.


Abstract
Background:
Foodborne pathogens, particularly antibiotic-resistant strains, pose a significant threat to public health globally.

Aim:
To explore the emergence and spread of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in the food chain, focusing on broiler chickens in wet markets.

Methods:
Specimens were inoculated into TBX medium supplemented with cefotaxime 4 ug/ml for selective isolation of ESBL-producing E. coli. Phenotypic and genotypic characterization of the isolates was performed, including species detection using PCR for E. coli identification with specific primers targeting the gene and a double diffusion synergy test for ESBL identification.

Results:
A total of 262/305 (86%) samples were confirmed positive for ESBL-producing E. coli from broiler chickens and free-range chickens. blaCTX-M were mostly identified among ESBL-producing E. coli from broiler chickens (91%) or free-range chicken (100%), followed by blaTEM 20% and 23%, respectively. There were no identified blaSHV genes. Among 50 ESBL producers, E. coli (31; 17.4% from broiler chickens and 19; 22.6% from free-range chicken) were identified with a co-incidence of blaCTX-M and blaTEM.

Conclusion:
There is an increased prevalence of ESBL-producing E. coli in environmentally contaminated foods. Hygiene and antibiotic use regulations for poultry farms should be improved. ESBL genes among chickens are mainly blaCTX-M (94%), followed by blaTEM (21%), which has a lower incidence; no blaSHV was detected.

Key words: Antimicrobial resistance, Chicken, Escherichia coli, ESBL, Food chain, Infectious disease


 
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How to Cite this Article
Pubmed Style

Kuntaman K, Masfufatun M, Wibisono FJ, Sarassari R, Setyorini W, Tania POA, Shirakawa T. Assessment of the environmental transmission dynamics of Escherichia coli-producing ESBL in wet market broiler chickens: A high-burden food safety concern. Open Vet J. 2025; 15(5): 2230-2237. doi:10.5455/OVJ.2025.v15.i5.40

Web Style

Kuntaman K, Masfufatun M, Wibisono FJ, Sarassari R, Setyorini W, Tania POA, Shirakawa T. Assessment of the environmental transmission dynamics of Escherichia coli-producing ESBL in wet market broiler chickens: A high-burden food safety concern. https://www.openveterinaryjournal.com/?mno=245867 [Access: June 06, 2025]. doi:10.5455/OVJ.2025.v15.i5.40

AMA (American Medical Association) Style

Kuntaman K, Masfufatun M, Wibisono FJ, Sarassari R, Setyorini W, Tania POA, Shirakawa T. Assessment of the environmental transmission dynamics of Escherichia coli-producing ESBL in wet market broiler chickens: A high-burden food safety concern. Open Vet J. 2025; 15(5): 2230-2237. doi:10.5455/OVJ.2025.v15.i5.40


Vancouver/ICMJE Style

Kuntaman K, Masfufatun M, Wibisono FJ, Sarassari R, Setyorini W, Tania POA, Shirakawa T. Assessment of the environmental transmission dynamics of Escherichia coli-producing ESBL in wet market broiler chickens: A high-burden food safety concern. Open Vet J. (2025), [cited June 06, 2025]; 15(5): 2230-2237. doi:10.5455/OVJ.2025.v15.i5.40


Harvard Style

Kuntaman, K., Masfufatun, . M., Wibisono, . F. J., Sarassari, . R., Setyorini, . W., Tania, . P. O. A. & Shirakawa, . T. (2025) Assessment of the environmental transmission dynamics of Escherichia coli-producing ESBL in wet market broiler chickens: A high-burden food safety concern. Open Vet J, 15 (5), 2230-2237. doi:10.5455/OVJ.2025.v15.i5.40


Turabian Style

Kuntaman, Kuntaman, Masfufatun Masfufatun, Freshinta Jellia Wibisono, Rosantia Sarassari, Wahyu Setyorini, Putu Oky Ari Tania, and Toshiro Shirakawa. 2025. Assessment of the environmental transmission dynamics of Escherichia coli-producing ESBL in wet market broiler chickens: A high-burden food safety concern. Open Veterinary Journal, 15 (5), 2230-2237. doi:10.5455/OVJ.2025.v15.i5.40


Chicago Style

Kuntaman, Kuntaman, Masfufatun Masfufatun, Freshinta Jellia Wibisono, Rosantia Sarassari, Wahyu Setyorini, Putu Oky Ari Tania, and Toshiro Shirakawa. "Assessment of the environmental transmission dynamics of Escherichia coli-producing ESBL in wet market broiler chickens: A high-burden food safety concern." Open Veterinary Journal 15 (2025), 2230-2237. doi:10.5455/OVJ.2025.v15.i5.40


MLA (The Modern Language Association) Style

Kuntaman, Kuntaman, Masfufatun Masfufatun, Freshinta Jellia Wibisono, Rosantia Sarassari, Wahyu Setyorini, Putu Oky Ari Tania, and Toshiro Shirakawa. "Assessment of the environmental transmission dynamics of Escherichia coli-producing ESBL in wet market broiler chickens: A high-burden food safety concern." Open Veterinary Journal 15.5 (2025), 2230-2237. Print. doi:10.5455/OVJ.2025.v15.i5.40


APA (American Psychological Association) Style

Kuntaman, K., Masfufatun, . M., Wibisono, . F. J., Sarassari, . R., Setyorini, . W., Tania, . P. O. A. & Shirakawa, . T. (2025) Assessment of the environmental transmission dynamics of Escherichia coli-producing ESBL in wet market broiler chickens: A high-burden food safety concern. Open Veterinary Journal, 15 (5), 2230-2237. doi:10.5455/OVJ.2025.v15.i5.40


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