E-ISSN 2218-6050 | ISSN 2226-4485
 
2025, Vol: 15, Issue: 5
15 / 5Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles
Required files to be uploaded

OVJ Manuscript Submissio Form

Research Article


The effect of cryoprotectant on the quality of post-warming goat oocytes by examining SOD levels

Meisa Zalfa Adisti, Widjiati Widjiati, Agus Sunarso, Epy Muhammad Luqman, Tri Wahyu Suprayogi, Sri Pantja Madyawati, Suzanita Utama, Aulia Firmawati.


Abstract
Background:
Vitrification of oocytes can lead to the formation of reactive oxygen species during the in vitro maturation and warming processes. In this study, there is a comparison between the use of commercial cryoprotectant and ethylene glycol + sucrose to observe the antioxidant activity that arises during oxidative stress, namely superoxide dismutase (SOD). SOD is produced to counteract free radicals that are damaging to cells; the higher the level of SOD in a cell, the further the cell is from damage.

Aim:
The aim of this study is to determine whether different cryoprotectant use can affect the intracellular SOD levels in oocytes.

Methods:
This study is an experimental laboratory study using goat oocytes divided into 3 treatment groups: control (K) goat oocytes without vitrification, Treatment 1 (P1) goat oocytes vitrified using a commercial cryoprotectant, and Treatment 2 (P2) goat oocytes vitrified using ethylene glycol and 1-M sucrose. All groups were vitrified for 1 week, followed by a warming process. Then, the SOD levels were calculated using SOD ELISA kit.

Results:
The results showed that this study has a significance level (p < 0.05) with the control group K having the lowest SOD levels and P2 having the highest SOD levels.

Conclusion:
Therefore, it can be concluded that the use of ethylene glycol as a cryoprotectant can maintain SOD levels in oocyte cells.

Key words: Ethylene Glycol, Oocyte, ROS, SOD


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Meisa Zalfa Adisti
Articles by Widjiati Widjiati
Articles by Agus Sunarso
Articles by Epy Muhammad Luqman
Articles by Tri Wahyu Suprayogi
Articles by Sri Pantja Madyawati
Articles by Suzanita Utama
Articles by Aulia Firmawati
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Adisti MZ, Widjiati W, Sunarso A, Luqman EM, Suprayogi TW, Madyawati SP, Utama S, Firmawati A. The effect of cryoprotectant on the quality of post-warming goat oocytes by examining SOD levels. Open Vet J. 2025; 15(5): 2004-2008. doi:10.5455/OVJ.2025.v15.i5.15

Web Style

Adisti MZ, Widjiati W, Sunarso A, Luqman EM, Suprayogi TW, Madyawati SP, Utama S, Firmawati A. The effect of cryoprotectant on the quality of post-warming goat oocytes by examining SOD levels. https://www.openveterinaryjournal.com/?mno=236174 [Access: June 05, 2025]. doi:10.5455/OVJ.2025.v15.i5.15

AMA (American Medical Association) Style

Adisti MZ, Widjiati W, Sunarso A, Luqman EM, Suprayogi TW, Madyawati SP, Utama S, Firmawati A. The effect of cryoprotectant on the quality of post-warming goat oocytes by examining SOD levels. Open Vet J. 2025; 15(5): 2004-2008. doi:10.5455/OVJ.2025.v15.i5.15


Vancouver/ICMJE Style

Adisti MZ, Widjiati W, Sunarso A, Luqman EM, Suprayogi TW, Madyawati SP, Utama S, Firmawati A. The effect of cryoprotectant on the quality of post-warming goat oocytes by examining SOD levels. Open Vet J. (2025), [cited June 05, 2025]; 15(5): 2004-2008. doi:10.5455/OVJ.2025.v15.i5.15


Harvard Style

Adisti, M. Z., Widjiati, . W., Sunarso, . A., Luqman, . E. M., Suprayogi, . T. W., Madyawati, . S. P., Utama, . S. & Firmawati, . A. (2025) The effect of cryoprotectant on the quality of post-warming goat oocytes by examining SOD levels. Open Vet J, 15 (5), 2004-2008. doi:10.5455/OVJ.2025.v15.i5.15


Turabian Style

Adisti, Meisa Zalfa, Widjiati Widjiati, Agus Sunarso, Epy Muhammad Luqman, Tri Wahyu Suprayogi, Sri Pantja Madyawati, Suzanita Utama, and Aulia Firmawati. 2025. The effect of cryoprotectant on the quality of post-warming goat oocytes by examining SOD levels. Open Veterinary Journal, 15 (5), 2004-2008. doi:10.5455/OVJ.2025.v15.i5.15


Chicago Style

Adisti, Meisa Zalfa, Widjiati Widjiati, Agus Sunarso, Epy Muhammad Luqman, Tri Wahyu Suprayogi, Sri Pantja Madyawati, Suzanita Utama, and Aulia Firmawati. "The effect of cryoprotectant on the quality of post-warming goat oocytes by examining SOD levels." Open Veterinary Journal 15 (2025), 2004-2008. doi:10.5455/OVJ.2025.v15.i5.15


MLA (The Modern Language Association) Style

Adisti, Meisa Zalfa, Widjiati Widjiati, Agus Sunarso, Epy Muhammad Luqman, Tri Wahyu Suprayogi, Sri Pantja Madyawati, Suzanita Utama, and Aulia Firmawati. "The effect of cryoprotectant on the quality of post-warming goat oocytes by examining SOD levels." Open Veterinary Journal 15.5 (2025), 2004-2008. Print. doi:10.5455/OVJ.2025.v15.i5.15


APA (American Psychological Association) Style

Adisti, M. Z., Widjiati, . W., Sunarso, . A., Luqman, . E. M., Suprayogi, . T. W., Madyawati, . S. P., Utama, . S. & Firmawati, . A. (2025) The effect of cryoprotectant on the quality of post-warming goat oocytes by examining SOD levels. Open Veterinary Journal, 15 (5), 2004-2008. doi:10.5455/OVJ.2025.v15.i5.15


Author LoginReviewer LoginAbout PublisherPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyTerms of UseLicense InformationCopyright Information

About Open Veterinary Journal

Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Owner, Founder and Editor-in-Chief

Open Veterinary Journal


Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

and


Eldaghayes Publisher

www.eldaghayes.com 

Email: ibrahim.eldaghayes@vetmed.edu.ly