E-ISSN 2218-6050 | ISSN 2226-4485
 
2024, Vol: 14, Issue: 3
14 / 3Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles
Required files to be uploaded

OVJ Manuscript Submission Form

Original Article


Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula

Aboubaker M. Garbaj, Samira A. Farag, Jihan A. Sherif, Aml F. Lawila, Hanan L. Eshamah, Salah M. Azwai, Fatim T. Gammoudi, Hesham T. Naas, Allaaeddin A. El-Salabi, Ibrahim M. Eldaghayes.


Abstract
Background:
Cronobacter species is the most significant foodborne pathogens in infant milk formula (IMF). These pathogens have been incriminated in sever form of neonatal meningitis, sepsis and necrotizing enterocolitis with high mortality rate.
Aim:
The current study was performed to demonstrate the effect of heat stress on Cronobacter spp. (C. sakazakii and C. pulveris) in reconstituted IMF (RIMF).
Methods:
The reconstituted formula was inoculated with five C. sakazakii isolates and four C. pulveris isolates separately. The nine isolates of Cronobacter spp. were heated in RIMF at 48, 52, 56, 60, 64 and 66°C. The D- and z-values were determined by using linear regression analysis.
Results:
The D˗values of all isolates of C. sakazakii (CS1, CS3, CS4, CS5 and CS6) at 48°C, 52°C, 56°C, 60°C, 64°C, 66°C ranged from 7.29 ˗ 23.47, 2.77˗15.50, 0.62˗1.04, 0.62˗1.02, 0.62˗1.00, 0.62˗1.00 min, respectively, while the z˗ values extended from 2.50˗4.28°C. The D˗ values of C. pulveris isolates (CP1, CP2, CP3, CP4) ranged from 7.60 ˗ 22.32, 1.42 ˗ 8.45, 0.62 ˗ 1.08, 0.62 ˗ 0.78, 0.62 ˗ 0.78, 0.62 ˗ 0.79 min. at 48°C, 52°C, 56°C, 60°C, 64°C, 66°C, respectively and the calculated z-values ranged from 3.33 ˗ 4.89°C.
Conclusion:
This study may contribute to improve the understanding of behavior of C. sakazakii and C. pulveris isolates in RIMF at various heat stress temperature and may participate in effective control of this pathogens in infant’s foods production.

Key words: Cronobacter sakazakii, Cronobacter pulveris, Thermo-tolerance, D˗ value, z˗ value, Infant milk formula


 
ARTICLE TOOLS
Abstract
PDF Fulltext
HTML Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Aboubaker M. Garbaj
Articles by Samira A. Farag
Articles by Jihan A. Sherif
Articles by Aml F. Lawila
Articles by Hanan L. Eshamah
Articles by Salah M. Azwai
Articles by Fatim T. Gammoudi
Articles by Hesham T. Naas
Articles by Allaaeddin A. El-Salabi
Articles by Ibrahim M. Eldaghayes
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Garbaj AM, Farag SA, Sherif JA, Lawila AF, Eshamah HL, Azwai SM, Gammoudi FT, Naas HT, El-Salabi AA, Eldaghayes IM. Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula. Open Vet J. 2023; 13(1): 108-113. doi:10.5455/OVJ.2023.v13.i1.11

Web Style

Garbaj AM, Farag SA, Sherif JA, Lawila AF, Eshamah HL, Azwai SM, Gammoudi FT, Naas HT, El-Salabi AA, Eldaghayes IM. Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula. https://www.openveterinaryjournal.com/?mno=138198 [Access: April 09, 2024]. doi:10.5455/OVJ.2023.v13.i1.11

AMA (American Medical Association) Style

Garbaj AM, Farag SA, Sherif JA, Lawila AF, Eshamah HL, Azwai SM, Gammoudi FT, Naas HT, El-Salabi AA, Eldaghayes IM. Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula. Open Vet J. 2023; 13(1): 108-113. doi:10.5455/OVJ.2023.v13.i1.11


Vancouver/ICMJE Style

Garbaj AM, Farag SA, Sherif JA, Lawila AF, Eshamah HL, Azwai SM, Gammoudi FT, Naas HT, El-Salabi AA, Eldaghayes IM. Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula. Open Vet J. (2023), [cited April 09, 2024]; 13(1): 108-113. doi:10.5455/OVJ.2023.v13.i1.11


Harvard Style

Garbaj, A. M., Farag, . S. A., Sherif, . J. A., Lawila, . A. F., Eshamah, . H. L., Azwai, . S. M., Gammoudi, . F. T., Naas, . H. T., El-Salabi, . A. A. & Eldaghayes, . I. M. (2023) Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula. Open Vet J, 13 (1), 108-113. doi:10.5455/OVJ.2023.v13.i1.11


Turabian Style

Garbaj, Aboubaker M., Samira A. Farag, Jihan A. Sherif, Aml F. Lawila, Hanan L. Eshamah, Salah M. Azwai, Fatim T. Gammoudi, Hesham T. Naas, Allaaeddin A. El-Salabi, and Ibrahim M. Eldaghayes. 2023. Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula. Open Veterinary Journal, 13 (1), 108-113. doi:10.5455/OVJ.2023.v13.i1.11


Chicago Style

Garbaj, Aboubaker M., Samira A. Farag, Jihan A. Sherif, Aml F. Lawila, Hanan L. Eshamah, Salah M. Azwai, Fatim T. Gammoudi, Hesham T. Naas, Allaaeddin A. El-Salabi, and Ibrahim M. Eldaghayes. "Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula." Open Veterinary Journal 13 (2023), 108-113. doi:10.5455/OVJ.2023.v13.i1.11


MLA (The Modern Language Association) Style

Garbaj, Aboubaker M., Samira A. Farag, Jihan A. Sherif, Aml F. Lawila, Hanan L. Eshamah, Salah M. Azwai, Fatim T. Gammoudi, Hesham T. Naas, Allaaeddin A. El-Salabi, and Ibrahim M. Eldaghayes. "Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula." Open Veterinary Journal 13.1 (2023), 108-113. Print. doi:10.5455/OVJ.2023.v13.i1.11


APA (American Psychological Association) Style

Garbaj, A. M., Farag, . S. A., Sherif, . J. A., Lawila, . A. F., Eshamah, . H. L., Azwai, . S. M., Gammoudi, . F. T., Naas, . H. T., El-Salabi, . A. A. & Eldaghayes, . I. M. (2023) Thermal tolerance of Cronobacter sakazakii and Cronobacter pulveris in reconstituted infant milk formula. Open Veterinary Journal, 13 (1), 108-113. doi:10.5455/OVJ.2023.v13.i1.11


Author LoginReviewer LoginAbout PublisherPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyTerms of UseLicense Information

About Open Veterinary Journal

Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Owner, Founder and Editor-in-Chief

Open Veterinary Journal, Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya


Email: ibrahim.eldaghayes@vetmed.edu.ly