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2024, Vol: 14, Issue: 3
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Rapid identification and genotyping of the honeybee pathogen Paenibacillus larvae by combining culturing and multiplex quantitative PCR

Hannes Beims , Martina Janke, Werner von der Ohe , Michael Steinert.


Cited By:14

Abstract
American Foulbrood (AFB) is a devastating disease of honey bee (Apis meilifera) larvae caused by the spore-forming, Gram-positive bacterium Paenibacillus larvae. In most countries, the law requires mandatory reporting of AFB to the veterinary authority. To speed up detection and genotyping of P. larvae spores, we compared different culturing protocols on Columbia sheep blood agar (CSA) and developed a new multiplex qPCR to distinguish between the two relevant P. larvae genotypes ERIC I and ERIC II. As confirmed by P. larvae reference strains and field isolates, the new identification and genotyping protocol halves the time of current workflows, lessens labour-intension, allows a higher throughput of samples for monitoring, and permits a faster intervention to prevent the spread of AFB.

Key words: American Foulbrood, Paenibacillus larvae, rapid detection, multiplex quantitative PCR, ERIC genotyping


 
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How to Cite this Article
Pubmed Style

HB, Janke M, WvdO, Steinert M, . Rapid identification and genotyping of the honeybee pathogen Paenibacillus larvae by combining culturing and multiplex quantitative PCR. Open Vet J. 2020; 10(1): 53-58. doi:10.4314/ovj.v10i1.9

Web Style

HB, Janke M, WvdO, Steinert M, . Rapid identification and genotyping of the honeybee pathogen Paenibacillus larvae by combining culturing and multiplex quantitative PCR. https://www.openveterinaryjournal.com/?mno=49844 [Access: April 11, 2024]. doi:10.4314/ovj.v10i1.9

AMA (American Medical Association) Style

HB, Janke M, WvdO, Steinert M, . Rapid identification and genotyping of the honeybee pathogen Paenibacillus larvae by combining culturing and multiplex quantitative PCR. Open Vet J. 2020; 10(1): 53-58. doi:10.4314/ovj.v10i1.9


Vancouver/ICMJE Style

HB, Janke M, WvdO, Steinert M, . Rapid identification and genotyping of the honeybee pathogen Paenibacillus larvae by combining culturing and multiplex quantitative PCR. Open Vet J. (2020), [cited April 11, 2024]; 10(1): 53-58. doi:10.4314/ovj.v10i1.9


Harvard Style

, H. B., Janke, M., , W. v. d. O. ., Steinert, M. & (2020) Rapid identification and genotyping of the honeybee pathogen Paenibacillus larvae by combining culturing and multiplex quantitative PCR. Open Vet J, 10 (1), 53-58. doi:10.4314/ovj.v10i1.9


Turabian Style

, Hannes Beims, Martina Janke, Werner von der Ohe, Michael Steinert, and . 2020. Rapid identification and genotyping of the honeybee pathogen Paenibacillus larvae by combining culturing and multiplex quantitative PCR. Open Veterinary Journal, 10 (1), 53-58. doi:10.4314/ovj.v10i1.9


Chicago Style

, Hannes Beims, Martina Janke, Werner von der Ohe, Michael Steinert, and . "Rapid identification and genotyping of the honeybee pathogen Paenibacillus larvae by combining culturing and multiplex quantitative PCR." Open Veterinary Journal 10 (2020), 53-58. doi:10.4314/ovj.v10i1.9


MLA (The Modern Language Association) Style

, Hannes Beims, Martina Janke, Werner von der Ohe, Michael Steinert, and . "Rapid identification and genotyping of the honeybee pathogen Paenibacillus larvae by combining culturing and multiplex quantitative PCR." Open Veterinary Journal 10.1 (2020), 53-58. Print. doi:10.4314/ovj.v10i1.9


APA (American Psychological Association) Style

, H. B., Janke, M., , W. v. d. O. ., Steinert, M. & (2020) Rapid identification and genotyping of the honeybee pathogen Paenibacillus larvae by combining culturing and multiplex quantitative PCR. Open Veterinary Journal, 10 (1), 53-58. doi:10.4314/ovj.v10i1.9


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