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2026, Vol: 16, Issue: 7
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Case Report


Histopathological and molecular evidence of systemic Sarcocystis spp. infection in a free-living Guira Cuckoo (Guira guira)

Douglas Aparecido Silva, Maria Izabel Pedra Sogari, Ana Angelita Sampaio Baptista, Italmar Teodorico Navarro, João Luis Garcia, Luiz Daniel De Barros, Ana Paula Frederico Rodrigues Loureiro Bracarense.


Abstract
Background:
Sarcocystis species are protozoans that require intermediate and definitive hosts to complete their life cycle. Several species of Sarcocystis can infect birds and cause severe lesions. This study aimed to report the anatomopathological and molecular findings compatible with Sarcocystis spp. in guira cuckoos (Guira guira).

Case Description:
Two guira cuckoos were found dead and submitted for necropsy. Tissues were collected, fixed in 10% buffered formalin, and processed according to routine procedures. The sections were stained with hematoxylin and eosin. Nested polymerase chain reaction (PCR) targeting the 18s ribosomal ribonucleico acid region was performed to detect Sarcocystis spp., followed by digestion with restriction enzymes. Macroscopically, the lungs were edematous and dark red. One animal had partially digested cockroaches in the gizzard. Microscopically, diffuse pulmonary hemorrhage was associated with schizonts compatible with Sarcocystis. Schizonts associated with lymphocytic infiltrates were detected in the liver, skeletal muscle, and muscle tissues of the proventriculus. Encephalitis and gliosis were observed in the brain of one animal. Deoxyribonucleic acid amplification detected products compatible with Sarcocysts spp. in the tongue, heart, liver, pectoral muscle, brain, and esophagus.

Conclusion:
Together, the histopathological and molecular findings confirm systemic sarcocystosis in guira cuckoos and support their involvement in the local transmission cycle, with the presence of cockroaches suggesting a potential epidemiological link in parasite dissemination.

Key words: Histopathology; PCR-RFLP; Sarcocystosis; Wild bird.


 
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How to Cite this Article
Pubmed Style

Silva DA, Sogari MIP, Baptista AAS, Navarro IT, Garcia JL, Barros LDD, . Histopathological and molecular evidence of systemic Sarcocystis spp. infection in a free-living Guira Cuckoo (Guira guira). Open Vet. J.. 2026; 16(7): 4992-4998. doi:10.5455/OVJ.2026.v16.i7.78

Web Style

Silva DA, Sogari MIP, Baptista AAS, Navarro IT, Garcia JL, Barros LDD, . Histopathological and molecular evidence of systemic Sarcocystis spp. infection in a free-living Guira Cuckoo (Guira guira). https://www.openveterinaryjournal.com/?mno=309029 [Access: July 27, 2026]. doi:10.5455/OVJ.2026.v16.i7.78

AMA (American Medical Association) Style

Silva DA, Sogari MIP, Baptista AAS, Navarro IT, Garcia JL, Barros LDD, . Histopathological and molecular evidence of systemic Sarcocystis spp. infection in a free-living Guira Cuckoo (Guira guira). Open Vet. J.. 2026; 16(7): 4992-4998. doi:10.5455/OVJ.2026.v16.i7.78


Vancouver/ICMJE Style

Silva DA, Sogari MIP, Baptista AAS, Navarro IT, Garcia JL, Barros LDD, . Histopathological and molecular evidence of systemic Sarcocystis spp. infection in a free-living Guira Cuckoo (Guira guira). Open Vet. J.. (2026), [cited July 27, 2026]; 16(7): 4992-4998. doi:10.5455/OVJ.2026.v16.i7.78


Harvard Style

Silva, D. A., Sogari, . M. I. P., Baptista, . A. A. S., Navarro, . I. T., Garcia, . J. L., Barros, . L. D. D. & (2026) Histopathological and molecular evidence of systemic Sarcocystis spp. infection in a free-living Guira Cuckoo (Guira guira). Open Vet. J., 16 (7), 4992-4998. doi:10.5455/OVJ.2026.v16.i7.78


Turabian Style

Silva, Douglas Aparecido, Maria Izabel Pedra Sogari, Ana Angelita Sampaio Baptista, Italmar Teodorico Navarro, João Luis Garcia, Luiz Daniel De Barros, and Ana Paula Frederico Rodrigues Loureiro Bracarense. 2026. Histopathological and molecular evidence of systemic Sarcocystis spp. infection in a free-living Guira Cuckoo (Guira guira). Open Veterinary Journal, 16 (7), 4992-4998. doi:10.5455/OVJ.2026.v16.i7.78


Chicago Style

Silva, Douglas Aparecido, Maria Izabel Pedra Sogari, Ana Angelita Sampaio Baptista, Italmar Teodorico Navarro, João Luis Garcia, Luiz Daniel De Barros, and Ana Paula Frederico Rodrigues Loureiro Bracarense. "Histopathological and molecular evidence of systemic Sarcocystis spp. infection in a free-living Guira Cuckoo (Guira guira)." Open Veterinary Journal 16 (2026), 4992-4998. doi:10.5455/OVJ.2026.v16.i7.78


MLA (The Modern Language Association) Style

Silva, Douglas Aparecido, Maria Izabel Pedra Sogari, Ana Angelita Sampaio Baptista, Italmar Teodorico Navarro, João Luis Garcia, Luiz Daniel De Barros, and Ana Paula Frederico Rodrigues Loureiro Bracarense. "Histopathological and molecular evidence of systemic Sarcocystis spp. infection in a free-living Guira Cuckoo (Guira guira)." Open Veterinary Journal 16.7 (2026), 4992-4998. Print. doi:10.5455/OVJ.2026.v16.i7.78


APA (American Psychological Association) Style

Silva, D. A., Sogari, . M. I. P., Baptista, . A. A. S., Navarro, . I. T., Garcia, . J. L., Barros, . L. D. D. & (2026) Histopathological and molecular evidence of systemic Sarcocystis spp. infection in a free-living Guira Cuckoo (Guira guira). Open Veterinary Journal, 16 (7), 4992-4998. doi:10.5455/OVJ.2026.v16.i7.78


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