E-ISSN 2218-6050 | ISSN 2226-4485
 
2026, Vol: 16, Issue: 6
16 / 6Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles
Required files to be uploaded

OVJ Manuscript Submissio Form
Title Page
mainarticle_template

Research Article


Rapid visual detection of Staphylococcus epidermidis in bovine mastitis milk using LAMP–lateral flow dipstick

Mingfeng Chu, Xiaoyu Chong, Wei Cheng, Huiying Zhang, Yuchen Liang, Mengke Si, Yiwei Wang, Junxue Qiu, Baolei Yang, Xuelong Chen, Yanping Qi, Haixia Wang.


Abstract
Background:
The use of Loop-mediated Isothermal Amplification (LAMP) technology in combination with Lateral Flow Dipstick (LFD) for the rapid detection of Staphylococcus epidermidis (S. epidermidis) offers a technical solution for early disease monitoring in dairy farms.

Aim:
This method is intended to enable early and accurate identification of S. epidermidis infections in dairy farms, facilitating timely intervention and treatment to reduce economic losses and ensure milk quality and safety.

Methods:
A pair of primers specific to the aaa gene of S. epidermidis was designed, and the aaa-FIP primer was 5'-end labeled with biotin for LAMP amplification, followed by hybridization with a 6-FAM-labeled probe, and the the result was read out on an LFD. The specificity, sensitivity, and clinical applicability of the method were evaluated.

Results:
That the optimal LAMP reaction conditions are 64℃ for 30 min, followed by 5 min of probe hybridization and colorimetric reaction on the LFD strip, enabling specific and intuitive detection of S. epidermidis with a minimum detection concentration of 1.93×10⁻⁶ copies/µL. This method demonstrated 100 times higher sensitivity than conventional PCR and showed 100% consistency when applied to 20 clinical samples suspected of S. epidermidis infection.

Conclusion:
This study pioneers the development of a LAMP-LFD method that is simple to operate and can visually detect S. epidermidis associated with bovine mastitis with high specificity and sensitivity, making it suitable for rapid on-site detection of early infections in grassroots farms and holding great promise for broad application.

Key words: Mastitis in dairy cows; Loop-mediated isothermal amplification; Lateral flow dipstick; Rapid test; Staphylococcus epidermidis.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
HTML Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Mingfeng Chu
Articles by Xiaoyu Chong
Articles by Wei Cheng
Articles by Huiying Zhang
Articles by Yuchen Liang
Articles by Mengke Si
Articles by Yiwei Wang
Articles by Junxue Qiu
Articles by Baolei Yang
Articles by Xuelong Chen
Articles by Yanping Qi
Articles by Haixia Wang
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Chu M, Chong X, Cheng W, Zhang H, Liang Y, Si M, Wang Y, Qiu J, Yang B, Chen X, Qi Y, Wang H. Rapid visual detection of Staphylococcus epidermidis in bovine mastitis milk using LAMP–lateral flow dipstick. Open Vet. J.. 2026; 16(3): 1636-1645. doi:10.5455/OVJ.2026.v16.i3.21

Web Style

Chu M, Chong X, Cheng W, Zhang H, Liang Y, Si M, Wang Y, Qiu J, Yang B, Chen X, Qi Y, Wang H. Rapid visual detection of Staphylococcus epidermidis in bovine mastitis milk using LAMP–lateral flow dipstick. https://www.openveterinaryjournal.com/?mno=288940 [Access: June 26, 2026]. doi:10.5455/OVJ.2026.v16.i3.21

AMA (American Medical Association) Style

Chu M, Chong X, Cheng W, Zhang H, Liang Y, Si M, Wang Y, Qiu J, Yang B, Chen X, Qi Y, Wang H. Rapid visual detection of Staphylococcus epidermidis in bovine mastitis milk using LAMP–lateral flow dipstick. Open Vet. J.. 2026; 16(3): 1636-1645. doi:10.5455/OVJ.2026.v16.i3.21


Vancouver/ICMJE Style

Chu M, Chong X, Cheng W, Zhang H, Liang Y, Si M, Wang Y, Qiu J, Yang B, Chen X, Qi Y, Wang H. Rapid visual detection of Staphylococcus epidermidis in bovine mastitis milk using LAMP–lateral flow dipstick. Open Vet. J.. (2026), [cited June 26, 2026]; 16(3): 1636-1645. doi:10.5455/OVJ.2026.v16.i3.21


Harvard Style

Chu, M., Chong, . X., Cheng, . W., Zhang, . H., Liang, . Y., Si, . M., Wang, . Y., Qiu, . J., Yang, . B., Chen, . X., Qi, . Y. & Wang, . H. (2026) Rapid visual detection of Staphylococcus epidermidis in bovine mastitis milk using LAMP–lateral flow dipstick. Open Vet. J., 16 (3), 1636-1645. doi:10.5455/OVJ.2026.v16.i3.21


Turabian Style

Chu, Mingfeng, Xiaoyu Chong, Wei Cheng, Huiying Zhang, Yuchen Liang, Mengke Si, Yiwei Wang, Junxue Qiu, Baolei Yang, Xuelong Chen, Yanping Qi, and Haixia Wang. 2026. Rapid visual detection of Staphylococcus epidermidis in bovine mastitis milk using LAMP–lateral flow dipstick. Open Veterinary Journal, 16 (3), 1636-1645. doi:10.5455/OVJ.2026.v16.i3.21


Chicago Style

Chu, Mingfeng, Xiaoyu Chong, Wei Cheng, Huiying Zhang, Yuchen Liang, Mengke Si, Yiwei Wang, Junxue Qiu, Baolei Yang, Xuelong Chen, Yanping Qi, and Haixia Wang. "Rapid visual detection of Staphylococcus epidermidis in bovine mastitis milk using LAMP–lateral flow dipstick." Open Veterinary Journal 16 (2026), 1636-1645. doi:10.5455/OVJ.2026.v16.i3.21


MLA (The Modern Language Association) Style

Chu, Mingfeng, Xiaoyu Chong, Wei Cheng, Huiying Zhang, Yuchen Liang, Mengke Si, Yiwei Wang, Junxue Qiu, Baolei Yang, Xuelong Chen, Yanping Qi, and Haixia Wang. "Rapid visual detection of Staphylococcus epidermidis in bovine mastitis milk using LAMP–lateral flow dipstick." Open Veterinary Journal 16.3 (2026), 1636-1645. Print. doi:10.5455/OVJ.2026.v16.i3.21


APA (American Psychological Association) Style

Chu, M., Chong, . X., Cheng, . W., Zhang, . H., Liang, . Y., Si, . M., Wang, . Y., Qiu, . J., Yang, . B., Chen, . X., Qi, . Y. & Wang, . H. (2026) Rapid visual detection of Staphylococcus epidermidis in bovine mastitis milk using LAMP–lateral flow dipstick. Open Veterinary Journal, 16 (3), 1636-1645. doi:10.5455/OVJ.2026.v16.i3.21


Author LoginReviewer LoginAbout PublisherOpen Access PolicyEditorial Review PolicyEditorial & Peer Review ProcessPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyProtection of Research Participants (Statement On Human And Animal Rights)Corrections, Retractions & Expressions of ConcernDigital Archiving & Preservation PoliciesTerms of UseLicense InformationCopyright Information

About Open Veterinary Journal

Open Veterinary Journal provides high quality peer review process for original research articles, review articles, short communications and case reports i ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes

Editor-in-Chief

Open Veterinary Journal

Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

Email: ibrahim.eldaghayes@vetmed.edu.ly