E-ISSN 2218-6050 | ISSN 2226-4485
 
2025, Vol: 15, Issue: 12
15 / 12Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles
Required files to be uploaded

OVJ Manuscript Submissio Form
Title Page
mainarticle_template

Research Article


Immunomodulatory effects of dietary chaste tree (Vitex agnus-castus L.) extract in rainbow trout (Oncorhynchus mykiss)

Mohamed Omar Abdalla Salem, Masoud A. S. Lakwani.


Abstract
Background:
The exploration of natural dietary supplements to enhance immune function and disease resistance in aquaculture is of significant interest.

Aim:
This study aimed to investigate the capacity of dietary Vitex agnus-castus L. (Chaste Tree; CT) extract to modulate immune function in rainbow trout (Oncorhynchus mykiss).

Methods:
Fish were divided into four cohorts and maintained on isocaloric diets containing 0% (control), 0.5%, 1%, or 2% (w/w) CT extract for 60 days. Key innate immune markers, including blood respiratory burst activity and serum lysozyme activity, were quantified at weeks 4 and 8.

Results:
Supplementation with CT significantly increased (p < 0.05) respiratory burst activity in groups receiving 1% and 2% CT relative to controls. After 8 weeks of feeding, serum lysozyme activity was markedly elevated in fish fed 0.5% and 1% CT.

Conclusion:
These findings indicate that V. agnus-castus extract effectively potentiates crucial innate immune responses in rainbow trout, supporting its prospective application as a functional dietary supplement to enhance health status and disease resistance in aquaculture.

Key words: Vitex agnus-castus L.; Immunostimulant; Respiratory burst; Oncorhynchus mykiss; Aquaculture.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Mohamed Omar Abdalla Salem
Articles by Masoud A. S. Lakwani
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Salem MOA, Lakwani MAS. Immunomodulatory effects of dietary chaste tree (Vitex agnus-castus L.) extract in rainbow trout (Oncorhynchus mykiss). Open Vet. J.. 2025; 15(12): 6851-6855. doi:10.5455/OVJ.2025.v15.i12.65

Web Style

Salem MOA, Lakwani MAS. Immunomodulatory effects of dietary chaste tree (Vitex agnus-castus L.) extract in rainbow trout (Oncorhynchus mykiss). https://www.openveterinaryjournal.com/?mno=274084 [Access: January 10, 2026]. doi:10.5455/OVJ.2025.v15.i12.65

AMA (American Medical Association) Style

Salem MOA, Lakwani MAS. Immunomodulatory effects of dietary chaste tree (Vitex agnus-castus L.) extract in rainbow trout (Oncorhynchus mykiss). Open Vet. J.. 2025; 15(12): 6851-6855. doi:10.5455/OVJ.2025.v15.i12.65


Vancouver/ICMJE Style

Salem MOA, Lakwani MAS. Immunomodulatory effects of dietary chaste tree (Vitex agnus-castus L.) extract in rainbow trout (Oncorhynchus mykiss). Open Vet. J.. (2025), [cited January 10, 2026]; 15(12): 6851-6855. doi:10.5455/OVJ.2025.v15.i12.65


Harvard Style

Salem, M. O. A. & Lakwani, . M. A. S. (2025) Immunomodulatory effects of dietary chaste tree (Vitex agnus-castus L.) extract in rainbow trout (Oncorhynchus mykiss). Open Vet. J., 15 (12), 6851-6855. doi:10.5455/OVJ.2025.v15.i12.65


Turabian Style

Salem, Mohamed Omar Abdalla, and Masoud A. S. Lakwani. 2025. Immunomodulatory effects of dietary chaste tree (Vitex agnus-castus L.) extract in rainbow trout (Oncorhynchus mykiss). Open Veterinary Journal, 15 (12), 6851-6855. doi:10.5455/OVJ.2025.v15.i12.65


Chicago Style

Salem, Mohamed Omar Abdalla, and Masoud A. S. Lakwani. "Immunomodulatory effects of dietary chaste tree (Vitex agnus-castus L.) extract in rainbow trout (Oncorhynchus mykiss)." Open Veterinary Journal 15 (2025), 6851-6855. doi:10.5455/OVJ.2025.v15.i12.65


MLA (The Modern Language Association) Style

Salem, Mohamed Omar Abdalla, and Masoud A. S. Lakwani. "Immunomodulatory effects of dietary chaste tree (Vitex agnus-castus L.) extract in rainbow trout (Oncorhynchus mykiss)." Open Veterinary Journal 15.12 (2025), 6851-6855. Print. doi:10.5455/OVJ.2025.v15.i12.65


APA (American Psychological Association) Style

Salem, M. O. A. & Lakwani, . M. A. S. (2025) Immunomodulatory effects of dietary chaste tree (Vitex agnus-castus L.) extract in rainbow trout (Oncorhynchus mykiss). Open Veterinary Journal, 15 (12), 6851-6855. doi:10.5455/OVJ.2025.v15.i12.65


Author LoginReviewer LoginAbout PublisherPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyTerms of UseLicense InformationCopyright Information

About Open Veterinary Journal

Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Owner, Founder and Editor-in-Chief

Open Veterinary Journal


Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

and


Eldaghayes Publisher

www.eldaghayes.com 

Emails: ibrahim.eldaghayes@vetmed.edu.ly

info@eldaghayes.com