Abstract
This letter is a response to the recent article on the morphological and molecular identification of microfilariae in chickens in Northeastern Thailand. We recommend the authors for their important contributions to avian parasitology and offer additional perspectives on molecular techniques, sex-based infection patterns, pathological implications, and host specificity. Emphasis is placed on potential improvements in diagnostic methods and suggestions for future research directions to enhance understanding of filarial infections in poultry and their broader One Health relevance.

Key words: Eufilaria, Filariasis, Backyard chickens, Molecular detection, Avian parasites, Host specificity, One Health