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Research Article


Isolation and molecular identification of multidrug-resistant Escherichia coli strains isolated from mastitic cows in Egypt

Yumna Elsobky, Ibrahim M. Rabah, Walid S. Mousa, Khaled Sabbah, Mohamed A. Nayel, Ahmed M. Elsify, Asmaa A. Elgendy, Ahmed A. Zaghawa, Akram A. Salam, Ashraf M. Abu-seida, Abdulrahman Abdulkarim, Mohamed M. Elkamshishi.


Abstract
Background:
Mastitis is a common disease that affects the dairy sector globally because it not only impacts animal welfare but can also lead to significant financial losses.

Aim:
This study examined the phenotypic and genotypic profiles of the multidrug-resistant (MDR) Escherichia coli (E. coli) strains that were isolated from mastitic cows in Egypt to detect their pattern of antibiotic resistance.

Methods:
Four-hundred native breed lactating cows were evaluated to identify clinical and subclinical mastitis. A total of 100 mastitic milk samples (64 from clinical mastitis and 36 from subclinical mastitis) were collected for phenotypic isolation and identification of coliform bacteria. E. coli isolates were identified through their morphological features, Gram staining, and biochemical tests. The identified E. coli strains were examined against various antibiotics using disk diffusion methods. All E. coli strains were analyzed for the antibiotic resistance genes aadA, blaTEM, tetA, sul1, and qnrA using PCR.

Results:
Among 400 examined dairy cows, the prevalences of clinical and subclinical mastitis were 16% and 9%, respectively. Bacteriological isolation of coliform bacteria from mastitic milk samples revealed that E. coli was the most prevalent bacterium. Among 10 isolates of biochemically verified E. coli strains, 8 (80%) were MDR across 6 distinct classes of antibiotics. All recovered E. coli strains exhibited higher resistance to Amoxicillin, Cefotaxime, Sulphamethaxzole/Trimethoprim, and Tetracycline. High susceptibility was noticed to Ciprofloxaccin, Amoxicillin+Clavulinic, Streptomycin, Gentamicin, Chloramphenicol, and Colistin. The blaTEM gene is among the most common antibiotic resistance genes found in E. coli isolates (100%). Furthermore, the genotypes encoding resistance to Tetracycline (tetA), Streptomycin (aadA), and Sulfonamides (sulI) were 50%, 40%, and 50%, respectively.

Conclusion:
MDR pathogenic E. coli strains are common in mastitic dairy cows in Egypt, and preventive actions must be implemented to avoid serious public health concerns.

Key words: Antibiogram, Coliform bacteria, Dairy cows, Mastitis, Milk, Resistance genes


 
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How to Cite this Article
Pubmed Style

Elsobky Y, Rabah IM, Mousa WS, Sabbah K, Nayel MA, Elsify AM, Elgendy AA, Zaghawa AA, Salam AA, Abu-seida AM, Abdulkarim A, Elkamshishi MM. Isolation and molecular identification of multidrug-resistant Escherichia coli strains isolated from mastitic cows in Egypt. Open Vet J. 2025; 15(5): 2094-2102. doi:10.5455/OVJ.2025.v15.i5.27

Web Style

Elsobky Y, Rabah IM, Mousa WS, Sabbah K, Nayel MA, Elsify AM, Elgendy AA, Zaghawa AA, Salam AA, Abu-seida AM, Abdulkarim A, Elkamshishi MM. Isolation and molecular identification of multidrug-resistant Escherichia coli strains isolated from mastitic cows in Egypt. https://www.openveterinaryjournal.com/?mno=239986 [Access: June 06, 2025]. doi:10.5455/OVJ.2025.v15.i5.27

AMA (American Medical Association) Style

Elsobky Y, Rabah IM, Mousa WS, Sabbah K, Nayel MA, Elsify AM, Elgendy AA, Zaghawa AA, Salam AA, Abu-seida AM, Abdulkarim A, Elkamshishi MM. Isolation and molecular identification of multidrug-resistant Escherichia coli strains isolated from mastitic cows in Egypt. Open Vet J. 2025; 15(5): 2094-2102. doi:10.5455/OVJ.2025.v15.i5.27


Vancouver/ICMJE Style

Elsobky Y, Rabah IM, Mousa WS, Sabbah K, Nayel MA, Elsify AM, Elgendy AA, Zaghawa AA, Salam AA, Abu-seida AM, Abdulkarim A, Elkamshishi MM. Isolation and molecular identification of multidrug-resistant Escherichia coli strains isolated from mastitic cows in Egypt. Open Vet J. (2025), [cited June 06, 2025]; 15(5): 2094-2102. doi:10.5455/OVJ.2025.v15.i5.27


Harvard Style

Elsobky, Y., Rabah, . I. M., Mousa, . W. S., Sabbah, . K., Nayel, . M. A., Elsify, . A. M., Elgendy, . A. A., Zaghawa, . A. A., Salam, . A. A., Abu-seida, . A. M., Abdulkarim, . A. & Elkamshishi, . M. M. (2025) Isolation and molecular identification of multidrug-resistant Escherichia coli strains isolated from mastitic cows in Egypt. Open Vet J, 15 (5), 2094-2102. doi:10.5455/OVJ.2025.v15.i5.27


Turabian Style

Elsobky, Yumna, Ibrahim M. Rabah, Walid S. Mousa, Khaled Sabbah, Mohamed A. Nayel, Ahmed M. Elsify, Asmaa A. Elgendy, Ahmed A. Zaghawa, Akram A. Salam, Ashraf M. Abu-seida, Abdulrahman Abdulkarim, and Mohamed M. Elkamshishi. 2025. Isolation and molecular identification of multidrug-resistant Escherichia coli strains isolated from mastitic cows in Egypt. Open Veterinary Journal, 15 (5), 2094-2102. doi:10.5455/OVJ.2025.v15.i5.27


Chicago Style

Elsobky, Yumna, Ibrahim M. Rabah, Walid S. Mousa, Khaled Sabbah, Mohamed A. Nayel, Ahmed M. Elsify, Asmaa A. Elgendy, Ahmed A. Zaghawa, Akram A. Salam, Ashraf M. Abu-seida, Abdulrahman Abdulkarim, and Mohamed M. Elkamshishi. "Isolation and molecular identification of multidrug-resistant Escherichia coli strains isolated from mastitic cows in Egypt." Open Veterinary Journal 15 (2025), 2094-2102. doi:10.5455/OVJ.2025.v15.i5.27


MLA (The Modern Language Association) Style

Elsobky, Yumna, Ibrahim M. Rabah, Walid S. Mousa, Khaled Sabbah, Mohamed A. Nayel, Ahmed M. Elsify, Asmaa A. Elgendy, Ahmed A. Zaghawa, Akram A. Salam, Ashraf M. Abu-seida, Abdulrahman Abdulkarim, and Mohamed M. Elkamshishi. "Isolation and molecular identification of multidrug-resistant Escherichia coli strains isolated from mastitic cows in Egypt." Open Veterinary Journal 15.5 (2025), 2094-2102. Print. doi:10.5455/OVJ.2025.v15.i5.27


APA (American Psychological Association) Style

Elsobky, Y., Rabah, . I. M., Mousa, . W. S., Sabbah, . K., Nayel, . M. A., Elsify, . A. M., Elgendy, . A. A., Zaghawa, . A. A., Salam, . A. A., Abu-seida, . A. M., Abdulkarim, . A. & Elkamshishi, . M. M. (2025) Isolation and molecular identification of multidrug-resistant Escherichia coli strains isolated from mastitic cows in Egypt. Open Veterinary Journal, 15 (5), 2094-2102. doi:10.5455/OVJ.2025.v15.i5.27


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