E-ISSN 2218-6050 | ISSN 2226-4485
 
2024, Vol: 14, Issue: 3
14 / 3Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles
Required files to be uploaded

OVJ Manuscript Submission Form

Original Article


Isolation and Identification of Mycoplasma mycoides subsp. mycoides in Cattle from South-East Nigeria.

Kingsley Chineto Anyika, Solomon Olu Okaiyeto, Anthony KB Sackey, Clara Nna Kwanashie, Pam D Luka, Paul Ankeli.


Abstract
Background: Mycoplasma mycoides subsp. mycoides is the causative organism of contagious bovine pleuropneumonia (CBPP). It is a trans-boundary disease (TAD) and endemic in Nigeria causing serious financial loss.
Aim: This study was undertaken to isolate and confirm the presence of Mycoplasma mycoides subsp. mycoides (Mmm) in cattle from three selected South-Eastern states of Nigeria.
Method: A total of Ninety (90) bovine samples (25 pleural fluids and 65 lung tissues) suggestive of CBPP were collected from different abattoirs in the three selected South-eastern states of Nigeria (Anambra, Enugu and Imo), for the isolation of Mmm cultural method was employed, whereas for confirmation Polymerase Chain Reaction (PCR) approach was used. The collected samples were cultured on Pleuropneumonia like organism (PPLO) agar according to specific protocols.
Results: Twenty five (25) of the samples (lungs and pleural fluid) were positive for Mmm on PPLO agar giving an isolation rate of 27.7%. Only twenty one (21) of the isolates were further confirmed using PCR. The PCR amplification of the isolates produced a product of 1.1kbp which is specific for Mmm. No positive isolates were recovered from Imo state.
Conclusion: This study confirms the presence Mmm the causative organism of CBPP in South east Nigeria. It is recommended that active surveillance and vaccination protocol should be undertaken in the region for the control and prevention of this disease.

Key words: Cattle; CBPP; Mycoplasma mycoides subsp. mycoides; Polymerase chain reaction


 
ARTICLE TOOLS
Abstract
PDF Fulltext
HTML Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Kingsley Chineto Anyika
Articles by Solomon Olu Okaiyeto
Articles by Anthony KB Sackey
Articles by Clara Nna Kwanashie
Articles by Pam D Luka
Articles by Paul Ankeli
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Anyika KC, Okaiyeto SO, Sackey AK, Kwanashie CN, Luka PD, Ankeli P. Isolation and Identification of Mycoplasma mycoides subsp. mycoides in Cattle from South-East Nigeria.. Open Vet J. 2021; 11(1): 174-179. doi:10.5455/OVJ.v11.i1.25

Web Style

Anyika KC, Okaiyeto SO, Sackey AK, Kwanashie CN, Luka PD, Ankeli P. Isolation and Identification of Mycoplasma mycoides subsp. mycoides in Cattle from South-East Nigeria.. https://www.openveterinaryjournal.com/?mno=21657 [Access: April 22, 2024]. doi:10.5455/OVJ.v11.i1.25

AMA (American Medical Association) Style

Anyika KC, Okaiyeto SO, Sackey AK, Kwanashie CN, Luka PD, Ankeli P. Isolation and Identification of Mycoplasma mycoides subsp. mycoides in Cattle from South-East Nigeria.. Open Vet J. 2021; 11(1): 174-179. doi:10.5455/OVJ.v11.i1.25


Vancouver/ICMJE Style

Anyika KC, Okaiyeto SO, Sackey AK, Kwanashie CN, Luka PD, Ankeli P. Isolation and Identification of Mycoplasma mycoides subsp. mycoides in Cattle from South-East Nigeria.. Open Vet J. (2021), [cited April 22, 2024]; 11(1): 174-179. doi:10.5455/OVJ.v11.i1.25


Harvard Style

Anyika, K. C., Okaiyeto, . S. O., Sackey, . A. K., Kwanashie, . C. N., Luka, . P. D. & Ankeli, . P. (2021) Isolation and Identification of Mycoplasma mycoides subsp. mycoides in Cattle from South-East Nigeria.. Open Vet J, 11 (1), 174-179. doi:10.5455/OVJ.v11.i1.25


Turabian Style

Anyika, Kingsley Chineto, Solomon Olu Okaiyeto, Anthony KB Sackey, Clara Nna Kwanashie, Pam D Luka, and Paul Ankeli. 2021. Isolation and Identification of Mycoplasma mycoides subsp. mycoides in Cattle from South-East Nigeria.. Open Veterinary Journal, 11 (1), 174-179. doi:10.5455/OVJ.v11.i1.25


Chicago Style

Anyika, Kingsley Chineto, Solomon Olu Okaiyeto, Anthony KB Sackey, Clara Nna Kwanashie, Pam D Luka, and Paul Ankeli. "Isolation and Identification of Mycoplasma mycoides subsp. mycoides in Cattle from South-East Nigeria.." Open Veterinary Journal 11 (2021), 174-179. doi:10.5455/OVJ.v11.i1.25


MLA (The Modern Language Association) Style

Anyika, Kingsley Chineto, Solomon Olu Okaiyeto, Anthony KB Sackey, Clara Nna Kwanashie, Pam D Luka, and Paul Ankeli. "Isolation and Identification of Mycoplasma mycoides subsp. mycoides in Cattle from South-East Nigeria.." Open Veterinary Journal 11.1 (2021), 174-179. Print. doi:10.5455/OVJ.v11.i1.25


APA (American Psychological Association) Style

Anyika, K. C., Okaiyeto, . S. O., Sackey, . A. K., Kwanashie, . C. N., Luka, . P. D. & Ankeli, . P. (2021) Isolation and Identification of Mycoplasma mycoides subsp. mycoides in Cattle from South-East Nigeria.. Open Veterinary Journal, 11 (1), 174-179. doi:10.5455/OVJ.v11.i1.25


Author LoginReviewer LoginAbout PublisherPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyTerms of UseLicense Information

About Open Veterinary Journal

Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Owner, Founder and Editor-in-Chief

Open Veterinary Journal, Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya


Email: ibrahim.eldaghayes@vetmed.edu.ly