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Research Article


Quality and kinematic characteristics of Bali bulls frozen semen with purified green tea extract epigallocatechin-3-gallate antioxidant addition in diluent

Ragil Angga Prastiya, Trilas Sardjito, Amung Logam Saputro, Darmawan Setia Budi, Styuderil Imaniro Maxdhameta, Elis Sulistiyawati, Deny Sulistyowati, Anny Amaliya, Samira Musa Sasi, Nining Haryuni.


Abstract
Background:
The preservation of semen quality and kinematic characteristics during cryopreservation is crucial for the reproductive success and genetic management of livestock, particularly in Bali bulls.
Aim:
This study aimed to investigate the effect of adding purified green tea extract antioxidant Epigallocatechin-3-gallate (EGCG) in tris egg yolk diluent on the quality and kinematic characteristics of frozen semen from Bali bulls.
Methods:
Fresh and frozen semen samples were obtained from Bali bull and divided into four different treatment groups. P0 contained semen samples + diluent, while P1 to P3 consisted of semen samples + diluent supplemented with EGCG levels of 0.1 mg/100 mL, 0.15 mg/100 mL, and 0.2 mg/100 mL, respectively. Data were analyzed using One-way ANOVA and followed by Duncan's test if significant differences were found (p<0.05). Parameters observed included the assessment of fresh semen quality, kinematic analysis, post-thawing sperm viability and abnormality.
Results:
The results indicated that the assessment of fresh semen quality showed macroscopic and microscopic semen quality according to SNI 4869-1:2021. Kinematic analysis revealed significant differences in DSL and STR parameters between P0 and P3 (p<0.05). EGCG supplementation also caused significant differences in motility between P0 and P3 (p<0.05). Viability and spermatozoa abnormality with EGCG supplementation did not show significant differences (p>0.05).
Conclusion:
The best results for motility, kinematics, and sperm morphology variables were found in P1 as it did not exhibit a decrease in motility, kinematics, and sperm morphology. Viability did not show significant differences between P1, P2, and P3, but the best results were found in P2 as it did not exhibit a decrease in viability with mean and standard deviation (66.84 ± 7.88). Abnormality variables also did not show significant differences between P1, P2, and P3, but the best results were found in P2 as it did not exhibit a decrease in abnormality with mean and standard deviation (23.80 ± 7.36).

Key words: Bali bull, EGCG, Kinematics, Livestock and gene bank, Sperm quality


 
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How to Cite this Article
Pubmed Style

Prastiya RA, Sardjito T, Saputro AL, Budi DS, Maxdhameta SI, Sulistiyawati E, Sulistyowati D, Amaliya A, Sasi SM, Haryuni N. Quality and kinematic characteristics of Bali bulls frozen semen with purified green tea extract epigallocatechin-3-gallate antioxidant addition in diluent. Open Vet J. 2024; 14(8): 2040-2048. doi:10.5455/OVJ.2024.v14.i8.33

Web Style

Prastiya RA, Sardjito T, Saputro AL, Budi DS, Maxdhameta SI, Sulistiyawati E, Sulistyowati D, Amaliya A, Sasi SM, Haryuni N. Quality and kinematic characteristics of Bali bulls frozen semen with purified green tea extract epigallocatechin-3-gallate antioxidant addition in diluent. https://www.openveterinaryjournal.com/?mno=205609 [Access: October 03, 2024]. doi:10.5455/OVJ.2024.v14.i8.33

AMA (American Medical Association) Style

Prastiya RA, Sardjito T, Saputro AL, Budi DS, Maxdhameta SI, Sulistiyawati E, Sulistyowati D, Amaliya A, Sasi SM, Haryuni N. Quality and kinematic characteristics of Bali bulls frozen semen with purified green tea extract epigallocatechin-3-gallate antioxidant addition in diluent. Open Vet J. 2024; 14(8): 2040-2048. doi:10.5455/OVJ.2024.v14.i8.33


Vancouver/ICMJE Style

Prastiya RA, Sardjito T, Saputro AL, Budi DS, Maxdhameta SI, Sulistiyawati E, Sulistyowati D, Amaliya A, Sasi SM, Haryuni N. Quality and kinematic characteristics of Bali bulls frozen semen with purified green tea extract epigallocatechin-3-gallate antioxidant addition in diluent. Open Vet J. (2024), [cited October 03, 2024]; 14(8): 2040-2048. doi:10.5455/OVJ.2024.v14.i8.33


Harvard Style

Prastiya, R. A., Sardjito, . T., Saputro, . A. L., Budi, . D. S., Maxdhameta, . S. I., Sulistiyawati, . E., Sulistyowati, . D., Amaliya, . A., Sasi, . S. M. & Haryuni, . N. (2024) Quality and kinematic characteristics of Bali bulls frozen semen with purified green tea extract epigallocatechin-3-gallate antioxidant addition in diluent. Open Vet J, 14 (8), 2040-2048. doi:10.5455/OVJ.2024.v14.i8.33


Turabian Style

Prastiya, Ragil Angga, Trilas Sardjito, Amung Logam Saputro, Darmawan Setia Budi, Styuderil Imaniro Maxdhameta, Elis Sulistiyawati, Deny Sulistyowati, Anny Amaliya, Samira Musa Sasi, and Nining Haryuni. 2024. Quality and kinematic characteristics of Bali bulls frozen semen with purified green tea extract epigallocatechin-3-gallate antioxidant addition in diluent. Open Veterinary Journal, 14 (8), 2040-2048. doi:10.5455/OVJ.2024.v14.i8.33


Chicago Style

Prastiya, Ragil Angga, Trilas Sardjito, Amung Logam Saputro, Darmawan Setia Budi, Styuderil Imaniro Maxdhameta, Elis Sulistiyawati, Deny Sulistyowati, Anny Amaliya, Samira Musa Sasi, and Nining Haryuni. "Quality and kinematic characteristics of Bali bulls frozen semen with purified green tea extract epigallocatechin-3-gallate antioxidant addition in diluent." Open Veterinary Journal 14 (2024), 2040-2048. doi:10.5455/OVJ.2024.v14.i8.33


MLA (The Modern Language Association) Style

Prastiya, Ragil Angga, Trilas Sardjito, Amung Logam Saputro, Darmawan Setia Budi, Styuderil Imaniro Maxdhameta, Elis Sulistiyawati, Deny Sulistyowati, Anny Amaliya, Samira Musa Sasi, and Nining Haryuni. "Quality and kinematic characteristics of Bali bulls frozen semen with purified green tea extract epigallocatechin-3-gallate antioxidant addition in diluent." Open Veterinary Journal 14.8 (2024), 2040-2048. Print. doi:10.5455/OVJ.2024.v14.i8.33


APA (American Psychological Association) Style

Prastiya, R. A., Sardjito, . T., Saputro, . A. L., Budi, . D. S., Maxdhameta, . S. I., Sulistiyawati, . E., Sulistyowati, . D., Amaliya, . A., Sasi, . S. M. & Haryuni, . N. (2024) Quality and kinematic characteristics of Bali bulls frozen semen with purified green tea extract epigallocatechin-3-gallate antioxidant addition in diluent. Open Veterinary Journal, 14 (8), 2040-2048. doi:10.5455/OVJ.2024.v14.i8.33


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