E-ISSN 2218-6050 | ISSN 2226-4485
 

Research Article


Mutation on Theileria species in cattle in Fallujah city, west region of Iraq

Dunya A.A. Mohammed Salih, Lina Adil Jebur, Noor A. Al-Taii, Wurood S. Ibadi.


Abstract
Background:
Theileriosis infection is a tick-borne disease that leads to global animal morbidity, mortality and economic losses.
Aim:
This study aimed to examine Theileriosis infection by traditional and molecular methods
Methods:
A total of 100 blood samples of local breed cows were examined by Giemsa smearing under a microscope, amplified-PCR in region 18SrRNA PCR at 600bp, and DNA sequencing analysis the genetic relationships.
Results:
The clinical signs showed that an elevated fever [40.3-41.5°C], an asymmetrical extension of superficially prescapular lymph nodes, lacrimation, icteric mucus membranes eyes, ecchymotic haemorrhages on sclera, dullness, lethargy, salivation, and ticks presence. Giemsa-stained blood smear and conventional PCR were revealed 24% and 73% for infection respectively. Phylogenetic tree was constructed with DNA sequencing analysis of accession number: OQ779946.1, OQ779947.1, OQ779949.1, OQ779952.1, OQ779953.1 and OQ779953.1, belonging to T. annulata; acc. nos.: OQ779950.1, OQ779951.1 belong to T. parva; acc. nos.: OQ779948.1, OQ779960.1 belongs to Theileria species; acc. no.: OQ779955.1, OQ779957.1, OQ779958.1 belong to T. sergenti; and OQ779956.1, OQ779959.1 belong to T. orientalis. Nucleotide substitutions of transversion and transitions occurred in 11 types containing gaps and missing are A>T; A>C; A>G; C>A; C>G; C>T; G>A; G>C; G>T; T>A; T>C for 254 positions in final dataset.
Conclusion:
This study showed theileriosis infection is highly endemic and represent a first investigation into use of molecular phylogeny to classify Theileria in Al-Fallujah City, West Region of Iraq.

Key words: Cattle, Fallujah city, Molecular study, Theileria spp


 
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How to Cite this Article
Pubmed Style

Salih DAM, Jebur LA, Al-Taii NA, Ibadi WS. Mutation on Theileria species in cattle in Fallujah city, west region of Iraq. Open Vet J. 2024; 14(9): 2261-2268. doi:10.5455/OVJ.2024.v14.i9.14


Web Style

Salih DAM, Jebur LA, Al-Taii NA, Ibadi WS. Mutation on Theileria species in cattle in Fallujah city, west region of Iraq. https://www.openveterinaryjournal.com/?mno=201393 [Access: October 03, 2024]. doi:10.5455/OVJ.2024.v14.i9.14


AMA (American Medical Association) Style

Salih DAM, Jebur LA, Al-Taii NA, Ibadi WS. Mutation on Theileria species in cattle in Fallujah city, west region of Iraq. Open Vet J. 2024; 14(9): 2261-2268. doi:10.5455/OVJ.2024.v14.i9.14



Vancouver/ICMJE Style

Salih DAM, Jebur LA, Al-Taii NA, Ibadi WS. Mutation on Theileria species in cattle in Fallujah city, west region of Iraq. Open Vet J. (2024), [cited October 03, 2024]; 14(9): 2261-2268. doi:10.5455/OVJ.2024.v14.i9.14



Harvard Style

Salih, D. A. M., Jebur, . L. A., Al-Taii, . N. A. & Ibadi, . W. S. (2024) Mutation on Theileria species in cattle in Fallujah city, west region of Iraq. Open Vet J, 14 (9), 2261-2268. doi:10.5455/OVJ.2024.v14.i9.14



Turabian Style

Salih, Dunya A.A. Mohammed, Lina Adil Jebur, Noor A. Al-Taii, and Wurood S. Ibadi. 2024. Mutation on Theileria species in cattle in Fallujah city, west region of Iraq. Open Veterinary Journal, 14 (9), 2261-2268. doi:10.5455/OVJ.2024.v14.i9.14



Chicago Style

Salih, Dunya A.A. Mohammed, Lina Adil Jebur, Noor A. Al-Taii, and Wurood S. Ibadi. "Mutation on Theileria species in cattle in Fallujah city, west region of Iraq." Open Veterinary Journal 14 (2024), 2261-2268. doi:10.5455/OVJ.2024.v14.i9.14



MLA (The Modern Language Association) Style

Salih, Dunya A.A. Mohammed, Lina Adil Jebur, Noor A. Al-Taii, and Wurood S. Ibadi. "Mutation on Theileria species in cattle in Fallujah city, west region of Iraq." Open Veterinary Journal 14.9 (2024), 2261-2268. Print. doi:10.5455/OVJ.2024.v14.i9.14



APA (American Psychological Association) Style

Salih, D. A. M., Jebur, . L. A., Al-Taii, . N. A. & Ibadi, . W. S. (2024) Mutation on Theileria species in cattle in Fallujah city, west region of Iraq. Open Veterinary Journal, 14 (9), 2261-2268. doi:10.5455/OVJ.2024.v14.i9.14