E-ISSN 2218-6050 | ISSN 2226-4485
 

Research Article


Centella asiatica extract ameliorates deoxygenation-induced neurological dysfunction in zebrafish larvae

Ariani Ariani, Husnul Khotimah, Arum Sulistyarini, Araisa Sabrina Daniaridevi.


Cited By:1

Abstract
Background:
Oxygen deprivation (OD) is a critical condition that can lead to brain damage and even death. Current hypoxia management approaches are limited in effectiveness. Centella asiatica, known for its neuroprotective properties, offers a potential alternative for OD treatment.
Aim:
This study aims to investigate the neuroprotective effects of Centella asiatica (CA) on the expression of Brain-Derived Neurotrophic Factor (BDNF) and Vesicular Glutamate Transporter 1 (VGLUT1) in zebrafish larvae under oxygen-deficient conditions.
Methods:
Zebrafish embryos were subjected to low oxygen levels (1.5 mg/L) 0-2 hours post-fertilization (hpf) until three days post-fertilization (dpf), simulating the early stages of OD. Subsequent treatment involved varying concentrations of CA (1.25-5 µg/ml) up to nine days post-fertilization. The expression levels of BDNF and VGLUT1 were measured using PCR methods. Statistical analysis was conducted using Two-way ANOVA to evaluate the impact of CA on the expression of BDNF and VGLUT1 in zebrafish larvae aged 3 and 9 dpf in oxygen-deprived conditions.
Results:
CA significantly influenced the expression of BDNF and VGLUT1 under oxygen deprivation (p<0.001). An increase in BDNF expression (p<0.001) and a decrease in VGLUT1 (p<0.01) were observed in zebrafish larvae experiencing OD and treated with CA. There was no significant difference in BDNF and VGLUT1 expression across age variations in zebrafish larvae at 3 dpf and 9 dpf in the treatment groups (p>0.05). CA concentration of 2.5 µg/ml effectively enhanced BDNF and reduced VGLUT1 in 3-9 dpf zebrafish larvae.
Conclusion:
CA demonstrates potential as a neuroprotective agent, modulating increased BDNF expression and reduced VGLUT1 under OD conditions. These findings lay a foundation for further research in developing therapies for oxygen deficiency.

Key words: Centella asiatica, Neuroprotective, Neurotoxicity, Neuroplasticity


 
ARTICLE TOOLS
Abstract
PDF Fulltext

About Open Veterinary Journal


Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information


All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Owner, Founder and Editor-in-Chief

Open Veterinary Journal


Faculty of Veterinary MedicineUniversity of Tripoli

P.O. Box 13662, Tripoli, Libya

and


Eldaghayes Publisher

www.eldagpublisher.com 

Email: ibrahim.eldaghayes@vetmed.edu.ly