E-ISSN 2218-6050 | ISSN 2226-4485
 

Research Article


Characterization of β-glucan obtained from Candida albicans of caprine mastitis

Saddam Hussein Mahmoud, Shaimaa Nabhan Yassein.


Abstract
Background:
Beta-glucan (β-glucan) is a polysaccharide containing β-glycosidic bonds that is an important structure part of different yeast cells.
Aim:
The purpose of the study is to characterize β-glucan that obtained from Candida albicans (C. albicans) isolated from caprine mastitis.
Methods:
The β-glucan was extracted by using utilizing an Alkaline-acidic extraction technique. The dry weight of extracted β-glucan was 7.47/150 gm with 4.98%.
Results:
The findings demonstrated that the extracted β-glucan had similarity in the primary peak 5.78 of liquid samples using the method of High-Performance Liquid Chromatography (HPLC) when compared to the standard form of β-glucan. However, Scanning Electron Microscopy (SEM) studies revealed which the standard of β-glucan was distinct in morphology but similar to β-glucan isolated from C. albicans in terms of particle sizes in the range of 1.60-2.65 m and the lack of cell wall traces. The findings of an investigation using Energy Dispersive X-ray Fluorescence Spectroscopy (EDS/EDX) of extracted and standard β-glucan, showed the principal elements discovered were carbon (C), oxygen (O), and nitrogen (N). Aluminum (Al), silicon (Si), nickel (Ni), and gold (Au) were also present, but in less amounts.
Conclusion:
The extracted β-glucan displayed a high degree of similarity and purity to the standard β-glucan, according to the findings of Fourier Transform Infrared Spectroscopy (FT-IR) research.

Key words: Subclinical mastitis, Mycotic mastitis, HPLC, Electron Microscopy, Iraq


 
ARTICLE TOOLS
Abstract
PDF Fulltext
HTML Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Saddam Hussein Mahmoud
Articles by Shaimaa Nabhan Yassein
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Mahmoud SH, Yassein SN. Characterization of β-glucan obtained from Candida albicans of caprine mastitis. Open Vet J. 2024; 14(5): 1269-1280. doi:10.5455/OVJ.2024.v14.i5.22


Web Style

Mahmoud SH, Yassein SN. Characterization of β-glucan obtained from Candida albicans of caprine mastitis. https://www.openveterinaryjournal.com/?mno=187356 [Access: July 13, 2024]. doi:10.5455/OVJ.2024.v14.i5.22


AMA (American Medical Association) Style

Mahmoud SH, Yassein SN. Characterization of β-glucan obtained from Candida albicans of caprine mastitis. Open Vet J. 2024; 14(5): 1269-1280. doi:10.5455/OVJ.2024.v14.i5.22



Vancouver/ICMJE Style

Mahmoud SH, Yassein SN. Characterization of β-glucan obtained from Candida albicans of caprine mastitis. Open Vet J. (2024), [cited July 13, 2024]; 14(5): 1269-1280. doi:10.5455/OVJ.2024.v14.i5.22



Harvard Style

Mahmoud, S. H. & Yassein, . S. N. (2024) Characterization of β-glucan obtained from Candida albicans of caprine mastitis. Open Vet J, 14 (5), 1269-1280. doi:10.5455/OVJ.2024.v14.i5.22



Turabian Style

Mahmoud, Saddam Hussein, and Shaimaa Nabhan Yassein. 2024. Characterization of β-glucan obtained from Candida albicans of caprine mastitis. Open Veterinary Journal, 14 (5), 1269-1280. doi:10.5455/OVJ.2024.v14.i5.22



Chicago Style

Mahmoud, Saddam Hussein, and Shaimaa Nabhan Yassein. "Characterization of β-glucan obtained from Candida albicans of caprine mastitis." Open Veterinary Journal 14 (2024), 1269-1280. doi:10.5455/OVJ.2024.v14.i5.22



MLA (The Modern Language Association) Style

Mahmoud, Saddam Hussein, and Shaimaa Nabhan Yassein. "Characterization of β-glucan obtained from Candida albicans of caprine mastitis." Open Veterinary Journal 14.5 (2024), 1269-1280. Print. doi:10.5455/OVJ.2024.v14.i5.22



APA (American Psychological Association) Style

Mahmoud, S. H. & Yassein, . S. N. (2024) Characterization of β-glucan obtained from Candida albicans of caprine mastitis. Open Veterinary Journal, 14 (5), 1269-1280. doi:10.5455/OVJ.2024.v14.i5.22