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Research Article


Kanamycin treated-ethylenediamine tetraacetic acid (EDTA)-dependent pseudothrombocytopenia in blood specimen of cats

Narissara Keawchana, Pirayu Rakwong, Pratthana Yongsakulchai.


Abstract
Background:
Pseudothrombocytopenia is a commonly obtained false negative result when analyzing feline platelet count by automated machine. It is related to ethylenediamine tetra-acetic acid (EDTA), a widely utilized anticoagulant in blood collection tube, resulting in EDTA-dependent pseudothrombocytopenia (EDTA-PTCP).
Aim:
To investigate whether treated with kanamycin enhanced the quantity of platelet aggregations in feline blood specimens collected using EDTA-PTCP.
Methods:
Thirty-one blood samples were obtained using EDTA tubes. The complete blood count was analyzed using automated Mindray-BC-5000Vet. Both Manual cell counts and thin blood smears were performed to estimate the amount of RBC, WBC and platelets as well as to evaluate the severity scores of platelet clumping, respectively. Comparisons were made between those pre-treated and treated with kanamycin in EDTA tube.
Results:
There were significantly different mean platelet counts in the samples before and after they were treated with kanamycin, both on automated (156.6  76.4 vs 260.3  115.5; p < 0.001) and manual (168.5  92.1 vs 262.8  119.6; p < 0.001) readings, with a 95% confidence interval (CI) of 0.19 (0.022–0.365).
Conclusion:
This study suggests that in clinical laboratory practice, kanamycin should be added to feline blood specimens with EDTA-PTCP.

Key words: EDTA-PTCP, Feline, Kanamycin, Platelet clumping, Pseudothrombocytopenia


 
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How to Cite this Article
Pubmed Style

Keawchana N, Rakwong P, Yongsakulchai P. Kanamycin treated-ethylenediamine tetraacetic acid (EDTA)-dependent pseudothrombocytopenia in blood specimen of cats. Open Vet J. 2024; 14(5): 1199-1205. doi:10.5455/OVJ.2024.v14.i5.15


Web Style

Keawchana N, Rakwong P, Yongsakulchai P. Kanamycin treated-ethylenediamine tetraacetic acid (EDTA)-dependent pseudothrombocytopenia in blood specimen of cats. https://www.openveterinaryjournal.com/?mno=186106 [Access: July 13, 2024]. doi:10.5455/OVJ.2024.v14.i5.15


AMA (American Medical Association) Style

Keawchana N, Rakwong P, Yongsakulchai P. Kanamycin treated-ethylenediamine tetraacetic acid (EDTA)-dependent pseudothrombocytopenia in blood specimen of cats. Open Vet J. 2024; 14(5): 1199-1205. doi:10.5455/OVJ.2024.v14.i5.15



Vancouver/ICMJE Style

Keawchana N, Rakwong P, Yongsakulchai P. Kanamycin treated-ethylenediamine tetraacetic acid (EDTA)-dependent pseudothrombocytopenia in blood specimen of cats. Open Vet J. (2024), [cited July 13, 2024]; 14(5): 1199-1205. doi:10.5455/OVJ.2024.v14.i5.15



Harvard Style

Keawchana, N., Rakwong, . P. & Yongsakulchai, . P. (2024) Kanamycin treated-ethylenediamine tetraacetic acid (EDTA)-dependent pseudothrombocytopenia in blood specimen of cats. Open Vet J, 14 (5), 1199-1205. doi:10.5455/OVJ.2024.v14.i5.15



Turabian Style

Keawchana, Narissara, Pirayu Rakwong, and Pratthana Yongsakulchai. 2024. Kanamycin treated-ethylenediamine tetraacetic acid (EDTA)-dependent pseudothrombocytopenia in blood specimen of cats. Open Veterinary Journal, 14 (5), 1199-1205. doi:10.5455/OVJ.2024.v14.i5.15



Chicago Style

Keawchana, Narissara, Pirayu Rakwong, and Pratthana Yongsakulchai. "Kanamycin treated-ethylenediamine tetraacetic acid (EDTA)-dependent pseudothrombocytopenia in blood specimen of cats." Open Veterinary Journal 14 (2024), 1199-1205. doi:10.5455/OVJ.2024.v14.i5.15



MLA (The Modern Language Association) Style

Keawchana, Narissara, Pirayu Rakwong, and Pratthana Yongsakulchai. "Kanamycin treated-ethylenediamine tetraacetic acid (EDTA)-dependent pseudothrombocytopenia in blood specimen of cats." Open Veterinary Journal 14.5 (2024), 1199-1205. Print. doi:10.5455/OVJ.2024.v14.i5.15



APA (American Psychological Association) Style

Keawchana, N., Rakwong, . P. & Yongsakulchai, . P. (2024) Kanamycin treated-ethylenediamine tetraacetic acid (EDTA)-dependent pseudothrombocytopenia in blood specimen of cats. Open Veterinary Journal, 14 (5), 1199-1205. doi:10.5455/OVJ.2024.v14.i5.15