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Research Article


Bovine umbilical mesenchymal stem cell-conditioned medium increased expression of GLUT-4 in pancreas of diabetic rats induced by nicotinamide-streptozotocin

Herawati Herawati, Hevi Wihadmadyatami, Muhammad Ali Zulfikar, Ricadonna Raissa, Made Bagus Auriva Mataram, Siti Kurniawati, Dyah Ayu O.A. Pratama.


Abstract
Background:
Diabetes is a degenerative disease associated with metabolic disorders. The majority of people have type 2 diabetes mellitus (DM) insulin resistance due to an unhealthy lifestyle. The development of DM treatment is also growing, one of which is using conditioned medium.
Aim:
This study aims to determine the effect of Bovine umbilical mesenchymal stem cell-conditioned medium (BUMSC-CM) on nicotinamide (NA) and streptozotocin (STZ) induced rats as an animal model of DM.
Methods:
The study began with the in vitro docking of Cholecalciferol with aldolase reductase and glucokinase. In the in vivo study, animal models were divided into five groups: group A (negative control), group B (diabetic rats), group C (NA+STZ+Metformin), group D (NA+STZ+ BUMSC-CM 0.2 ml/kg BW), and group E (NA+STZ+ BUMSC-CM 0.5 ml/kg BW). Blood sugar levels were checked, and BUMSC-CM was administered by intramuscular injection at four-day intervals for a duration of 16 days. Blood sugar levels were also sampled, and GLUT4 histochemical and immunohistochemical staining was performed.
Results:
The results showed that Cholecalciferol can bind to aldolase reductase ASP43 and TYR48 and bind to glucokinase at TYR214 with hydrogen bonds. BUMSC-CM administration was able to reduce blood sugar well. In addition, BUMSC-CM also helped repair the tissue structure of the pancreas damaged by inflammation from STZ administration.
Conclusion:
This study can be concluded that the administration of BUMSC-CM can be an alternative cell-free therapy for patients with DM.

Key words: Diabetes melitus, BUMSC-CM, Cholecalciferol, GLUT-4


 
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How to Cite this Article
Pubmed Style

Herawati H, Wihadmadyatami H, Zulfikar MA, Raissa R, Mataram MBA, Kurniawati S, Pratama DAO. Bovine umbilical mesenchymal stem cell-conditioned medium increased expression of GLUT-4 in pancreas of diabetic rats induced by nicotinamide-streptozotocin. Open Vet J. 2024; 14(8): 1761-1770. doi:10.5455/OVJ.2024.v14.i8.3

Web Style

Herawati H, Wihadmadyatami H, Zulfikar MA, Raissa R, Mataram MBA, Kurniawati S, Pratama DAO. Bovine umbilical mesenchymal stem cell-conditioned medium increased expression of GLUT-4 in pancreas of diabetic rats induced by nicotinamide-streptozotocin. https://www.openveterinaryjournal.com/?mno=179840 [Access: October 04, 2024]. doi:10.5455/OVJ.2024.v14.i8.3

AMA (American Medical Association) Style

Herawati H, Wihadmadyatami H, Zulfikar MA, Raissa R, Mataram MBA, Kurniawati S, Pratama DAO. Bovine umbilical mesenchymal stem cell-conditioned medium increased expression of GLUT-4 in pancreas of diabetic rats induced by nicotinamide-streptozotocin. Open Vet J. 2024; 14(8): 1761-1770. doi:10.5455/OVJ.2024.v14.i8.3


Vancouver/ICMJE Style

Herawati H, Wihadmadyatami H, Zulfikar MA, Raissa R, Mataram MBA, Kurniawati S, Pratama DAO. Bovine umbilical mesenchymal stem cell-conditioned medium increased expression of GLUT-4 in pancreas of diabetic rats induced by nicotinamide-streptozotocin. Open Vet J. (2024), [cited October 04, 2024]; 14(8): 1761-1770. doi:10.5455/OVJ.2024.v14.i8.3


Harvard Style

Herawati, H., Wihadmadyatami, . H., Zulfikar, . M. A., Raissa, . R., Mataram, . M. B. A., Kurniawati, . S. & Pratama, . D. A. O. (2024) Bovine umbilical mesenchymal stem cell-conditioned medium increased expression of GLUT-4 in pancreas of diabetic rats induced by nicotinamide-streptozotocin. Open Vet J, 14 (8), 1761-1770. doi:10.5455/OVJ.2024.v14.i8.3


Turabian Style

Herawati, Herawati, Hevi Wihadmadyatami, Muhammad Ali Zulfikar, Ricadonna Raissa, Made Bagus Auriva Mataram, Siti Kurniawati, and Dyah Ayu O.A. Pratama. 2024. Bovine umbilical mesenchymal stem cell-conditioned medium increased expression of GLUT-4 in pancreas of diabetic rats induced by nicotinamide-streptozotocin. Open Veterinary Journal, 14 (8), 1761-1770. doi:10.5455/OVJ.2024.v14.i8.3


Chicago Style

Herawati, Herawati, Hevi Wihadmadyatami, Muhammad Ali Zulfikar, Ricadonna Raissa, Made Bagus Auriva Mataram, Siti Kurniawati, and Dyah Ayu O.A. Pratama. "Bovine umbilical mesenchymal stem cell-conditioned medium increased expression of GLUT-4 in pancreas of diabetic rats induced by nicotinamide-streptozotocin." Open Veterinary Journal 14 (2024), 1761-1770. doi:10.5455/OVJ.2024.v14.i8.3


MLA (The Modern Language Association) Style

Herawati, Herawati, Hevi Wihadmadyatami, Muhammad Ali Zulfikar, Ricadonna Raissa, Made Bagus Auriva Mataram, Siti Kurniawati, and Dyah Ayu O.A. Pratama. "Bovine umbilical mesenchymal stem cell-conditioned medium increased expression of GLUT-4 in pancreas of diabetic rats induced by nicotinamide-streptozotocin." Open Veterinary Journal 14.8 (2024), 1761-1770. Print. doi:10.5455/OVJ.2024.v14.i8.3


APA (American Psychological Association) Style

Herawati, H., Wihadmadyatami, . H., Zulfikar, . M. A., Raissa, . R., Mataram, . M. B. A., Kurniawati, . S. & Pratama, . D. A. O. (2024) Bovine umbilical mesenchymal stem cell-conditioned medium increased expression of GLUT-4 in pancreas of diabetic rats induced by nicotinamide-streptozotocin. Open Veterinary Journal, 14 (8), 1761-1770. doi:10.5455/OVJ.2024.v14.i8.3


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