E-ISSN 2218-6050 | ISSN 2226-4485
 

Research Article


Asiatic acid increased locomotor and head width through inducing brain-derived neurotrophic factor in intrauterine hypoxia-exposed zebrafish

Ariani Ariani, Khotimah Husnul, Nurdiana Nurdiana, Rahayu Masruroh.


Abstract
Background:
Hypoxia ischemia leads to abnormal behavior and growth. Prenatal hypoxia also decreases brain adaptive potential, which can cause fatal effects such as cell death. Asiatic acid in Centella asiatica is a neuroprotector through antioxidant and antiinflammatory activities.
Aims:
This study aimed to analyze the effect of Asiatic acid as a neuroprotector against hypoxia during intrauterine development on locomotor activity, head width, and BDNF expression.
Methods:
The true experimental laboratory research used a posttest control-only design. Zebrafish embryos (Danio rerio) aged 0-2 dpf (days postfertilization) were exposed to hypoxia with oxygen levels reaching 1.5 mg/L. Then, Asiatic acid was administered at successive concentrations, namely, 0.36 μg/ml, 0.72 μg/ml, and 1.45 μg/ml, at 2 hpf (hours postfertilization), 3 dpf, 6 dpf, and 9 dpf. Head width, velocity activity and BDNF expression were observed.
Results:
Intrauterine hypoxia significantly decreased head width, velocity rate, and BDNF expression (<0.001). Administration of Asiatic acid at all concentrations and age 9 dpf to zebrafish larvae with intrauterine hypoxia exposure increased head width (p < 0.0001), velocity (p < 0.05), and relative mRNA expression of BDNF (p < 0.05).
Conclusion:
Asiatic acid is potentially neuroprotectiveto the brain in zebrafish larvae exposed to hypoxia during intrauterine development.

Key words: Asiatic acid, BDNF, Growth, Hypoxia, Neuron


 
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How to Cite this Article
Pubmed Style

Ariani A, Husnul K, Nurdiana N, Masruroh R. Asiatic acid increased locomotor and head width through inducing brain-derived neurotrophic factor in intrauterine hypoxia-exposed zebrafish. Open Vet J. 2023; 13(10): 1326-1333. doi:10.5455/OVJ.2023.v13.i10.12


Web Style

Ariani A, Husnul K, Nurdiana N, Masruroh R. Asiatic acid increased locomotor and head width through inducing brain-derived neurotrophic factor in intrauterine hypoxia-exposed zebrafish. https://www.openveterinaryjournal.com/?mno=164040 [Access: February 13, 2024]. doi:10.5455/OVJ.2023.v13.i10.12


AMA (American Medical Association) Style

Ariani A, Husnul K, Nurdiana N, Masruroh R. Asiatic acid increased locomotor and head width through inducing brain-derived neurotrophic factor in intrauterine hypoxia-exposed zebrafish. Open Vet J. 2023; 13(10): 1326-1333. doi:10.5455/OVJ.2023.v13.i10.12



Vancouver/ICMJE Style

Ariani A, Husnul K, Nurdiana N, Masruroh R. Asiatic acid increased locomotor and head width through inducing brain-derived neurotrophic factor in intrauterine hypoxia-exposed zebrafish. Open Vet J. (2023), [cited February 13, 2024]; 13(10): 1326-1333. doi:10.5455/OVJ.2023.v13.i10.12



Harvard Style

Ariani, A., Husnul, . K., Nurdiana, . N. & Masruroh, . R. (2023) Asiatic acid increased locomotor and head width through inducing brain-derived neurotrophic factor in intrauterine hypoxia-exposed zebrafish. Open Vet J, 13 (10), 1326-1333. doi:10.5455/OVJ.2023.v13.i10.12



Turabian Style

Ariani, Ariani, Khotimah Husnul, Nurdiana Nurdiana, and Rahayu Masruroh. 2023. Asiatic acid increased locomotor and head width through inducing brain-derived neurotrophic factor in intrauterine hypoxia-exposed zebrafish. Open Veterinary Journal, 13 (10), 1326-1333. doi:10.5455/OVJ.2023.v13.i10.12



Chicago Style

Ariani, Ariani, Khotimah Husnul, Nurdiana Nurdiana, and Rahayu Masruroh. "Asiatic acid increased locomotor and head width through inducing brain-derived neurotrophic factor in intrauterine hypoxia-exposed zebrafish." Open Veterinary Journal 13 (2023), 1326-1333. doi:10.5455/OVJ.2023.v13.i10.12



MLA (The Modern Language Association) Style

Ariani, Ariani, Khotimah Husnul, Nurdiana Nurdiana, and Rahayu Masruroh. "Asiatic acid increased locomotor and head width through inducing brain-derived neurotrophic factor in intrauterine hypoxia-exposed zebrafish." Open Veterinary Journal 13.10 (2023), 1326-1333. Print. doi:10.5455/OVJ.2023.v13.i10.12



APA (American Psychological Association) Style

Ariani, A., Husnul, . K., Nurdiana, . N. & Masruroh, . R. (2023) Asiatic acid increased locomotor and head width through inducing brain-derived neurotrophic factor in intrauterine hypoxia-exposed zebrafish. Open Veterinary Journal, 13 (10), 1326-1333. doi:10.5455/OVJ.2023.v13.i10.12