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Research Article


Molecular genotyping of Salmonella spp isolated from cheese samples of local stores in Al-Diwaniyah city, Iraq

Orooba Meteab Faja, Afrah Sabeeh Mhyson, Wisam Reheem Atiyah, Basima Jasim Mohammed, Azal Adnan.


Abstract
Background:
Food safety is an important subject that global cheese industry increases the awareness upon. This urges these economic sectors to elevate the level of research to minimize cheese contamination with pathogenic bacteria, such as Salmonella.
Aim:
Based on these merits, this study was conducted to genotype Salmonella spp isolated from cheese samples of local stores in Al-Diwaniyah City, Iraq.
Methods:
The study used 41 samples of local fresh unsalted white cheese in a selective-growth based isolation of Salmonella. These isolates were confirmed utilizing a slide-agglutination (SA) test and VITEK® 2 system (V2S). Then, the isolates were subjected to conventional PCR and sequencing techniques that both targeted the 16S rRNA gene. For subtyping, the Salmonella isolates were subjected to a random amplified polymorphic DNA (RAPD)-PCR method.
Results:
The results of both SA and V2S revealed the presence of 14 (34.2%) isolates of Salmonella spp. in the cheese samples. The PCR confirmed 6 (42.9%) of these isolates, which further were defined with close nucleotide similarity (98.03%) and (97.88%) to different world isolates, such as Salmonella enterica subsp. Arizonae and Salmonella enterica subsp. enterica serovar Typhi, respectively. The RAPD-PCR findings showed different fragments for all the tested isolates.
Conclusion:
The present study indicates that the samples of the local fresh unsalted white cheese contain different Salmonella genotypes, which could be originated from different contamination sources.

Key words: Cheese, Food-borne pathogens, RAPD-PCR, Salmonella, VITEK® 2


 
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How to Cite this Article
Pubmed Style

Faja OM, Mhyson AS, Atiyah WR, Mohammed BJ, Adnan A. Molecular genotyping of Salmonella spp isolated from cheese samples of local stores in Al-Diwaniyah city, Iraq. Open Vet J. 2023; 13(10): 1277-1282. doi:10.5455/OVJ.2023.v13.i10.6


Web Style

Faja OM, Mhyson AS, Atiyah WR, Mohammed BJ, Adnan A. Molecular genotyping of Salmonella spp isolated from cheese samples of local stores in Al-Diwaniyah city, Iraq. https://www.openveterinaryjournal.com/?mno=153433 [Access: February 14, 2024]. doi:10.5455/OVJ.2023.v13.i10.6


AMA (American Medical Association) Style

Faja OM, Mhyson AS, Atiyah WR, Mohammed BJ, Adnan A. Molecular genotyping of Salmonella spp isolated from cheese samples of local stores in Al-Diwaniyah city, Iraq. Open Vet J. 2023; 13(10): 1277-1282. doi:10.5455/OVJ.2023.v13.i10.6



Vancouver/ICMJE Style

Faja OM, Mhyson AS, Atiyah WR, Mohammed BJ, Adnan A. Molecular genotyping of Salmonella spp isolated from cheese samples of local stores in Al-Diwaniyah city, Iraq. Open Vet J. (2023), [cited February 14, 2024]; 13(10): 1277-1282. doi:10.5455/OVJ.2023.v13.i10.6



Harvard Style

Faja, O. M., Mhyson, . A. S., Atiyah, . W. R., Mohammed, . B. J. & Adnan, . A. (2023) Molecular genotyping of Salmonella spp isolated from cheese samples of local stores in Al-Diwaniyah city, Iraq. Open Vet J, 13 (10), 1277-1282. doi:10.5455/OVJ.2023.v13.i10.6



Turabian Style

Faja, Orooba Meteab, Afrah Sabeeh Mhyson, Wisam Reheem Atiyah, Basima Jasim Mohammed, and Azal Adnan. 2023. Molecular genotyping of Salmonella spp isolated from cheese samples of local stores in Al-Diwaniyah city, Iraq. Open Veterinary Journal, 13 (10), 1277-1282. doi:10.5455/OVJ.2023.v13.i10.6



Chicago Style

Faja, Orooba Meteab, Afrah Sabeeh Mhyson, Wisam Reheem Atiyah, Basima Jasim Mohammed, and Azal Adnan. "Molecular genotyping of Salmonella spp isolated from cheese samples of local stores in Al-Diwaniyah city, Iraq." Open Veterinary Journal 13 (2023), 1277-1282. doi:10.5455/OVJ.2023.v13.i10.6



MLA (The Modern Language Association) Style

Faja, Orooba Meteab, Afrah Sabeeh Mhyson, Wisam Reheem Atiyah, Basima Jasim Mohammed, and Azal Adnan. "Molecular genotyping of Salmonella spp isolated from cheese samples of local stores in Al-Diwaniyah city, Iraq." Open Veterinary Journal 13.10 (2023), 1277-1282. Print. doi:10.5455/OVJ.2023.v13.i10.6



APA (American Psychological Association) Style

Faja, O. M., Mhyson, . A. S., Atiyah, . W. R., Mohammed, . B. J. & Adnan, . A. (2023) Molecular genotyping of Salmonella spp isolated from cheese samples of local stores in Al-Diwaniyah city, Iraq. Open Veterinary Journal, 13 (10), 1277-1282. doi:10.5455/OVJ.2023.v13.i10.6