E-ISSN 2218-6050 | ISSN 2226-4485
 
2025, Vol: 15, Issue: 8
15 / 8Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles
Required files to be uploaded

OVJ Manuscript Submissio Form

Original Article


Identification of reference genes for expression studies in the liver, and spleen of laying hens housed in cage and cage-free systems

María Paula Herrera-sánchez, Kelly Johanna Lozano-villegas, Iang Schroniltgen Rondón-barragán, Roy Rodríguez-hernández.


Cited By:3

Abstract
Background:
The liver and spleen play a pivotal role in metabolism and immune response. During stress, neuroendocrine response induces changes in gene expression and its assessment demands the validation of the stability of the reference genes to perform relative gene expression experiments.
Aim:
The objective of this study was to determine the expression stability of four reference genes (GAPDH, ACTB, RNA18S, and HMBS) in the liver and spleen tissues from laying hens housed in conventional cage (CC) and cage-free (CF) egg production systems.
Methods:
Liver and spleen from Hy-Line Brown hens housed in CC and CF egg production systems were used. mRNA transcript levels were determined by qPCR and the gene expression stability was evaluated using geNorm, BestKeeper and NormFinder algorithms.
Results:
The most stable gene from liver tissue was ACTB in CC, CF and CC-CF group (overall data). In the spleen, the most stables genes were GAPDH (CC), HMBS (CF), and ACTB (CC-CF).
Conclusion:
The ACTB gene was the most stable gene in the liver, and GAPDH and HMBS genes were stable in spleen tissues that could be used for the normalization in qPCR experiments performed in liver and spleen tissues of laying hens housed CC and CF production systems.

Key words: Laying hens, Liver, mRNA, Reference genes, Spleen


 
ARTICLE TOOLS
Abstract
PDF Fulltext
HTML Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by María Paula Herrera-Sánchez
Articles by Kelly Johanna Lozano-Villegas
Articles by Iang Schroniltgen Rondón-Barragán
Articles by Roy Rodríguez-Hernández
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Herrera-sánchez MP, Lozano-villegas KJ, Rondón-barragán IS, Rodríguez-hernández R. Identification of reference genes for expression studies in the liver, and spleen of laying hens housed in cage and cage-free systems. Open Vet. J.. 2023; 13(3): 270-277. doi:10.5455/OVJ.2023.v13.i3.3

Web Style

Herrera-sánchez MP, Lozano-villegas KJ, Rondón-barragán IS, Rodríguez-hernández R. Identification of reference genes for expression studies in the liver, and spleen of laying hens housed in cage and cage-free systems. https://www.openveterinaryjournal.com/?mno=133560 [Access: September 01, 2025]. doi:10.5455/OVJ.2023.v13.i3.3

AMA (American Medical Association) Style

Herrera-sánchez MP, Lozano-villegas KJ, Rondón-barragán IS, Rodríguez-hernández R. Identification of reference genes for expression studies in the liver, and spleen of laying hens housed in cage and cage-free systems. Open Vet. J.. 2023; 13(3): 270-277. doi:10.5455/OVJ.2023.v13.i3.3


Vancouver/ICMJE Style

Herrera-sánchez MP, Lozano-villegas KJ, Rondón-barragán IS, Rodríguez-hernández R. Identification of reference genes for expression studies in the liver, and spleen of laying hens housed in cage and cage-free systems. Open Vet. J.. (2023), [cited September 01, 2025]; 13(3): 270-277. doi:10.5455/OVJ.2023.v13.i3.3


Harvard Style

Herrera-sánchez, M. P., Lozano-villegas, . K. J., Rondón-barragán, . I. S. & Rodríguez-hernández, . R. (2023) Identification of reference genes for expression studies in the liver, and spleen of laying hens housed in cage and cage-free systems. Open Vet. J., 13 (3), 270-277. doi:10.5455/OVJ.2023.v13.i3.3


Turabian Style

Herrera-sánchez, María Paula, Kelly Johanna Lozano-villegas, Iang Schroniltgen Rondón-barragán, and Roy Rodríguez-hernández. 2023. Identification of reference genes for expression studies in the liver, and spleen of laying hens housed in cage and cage-free systems. Open Veterinary Journal, 13 (3), 270-277. doi:10.5455/OVJ.2023.v13.i3.3


Chicago Style

Herrera-sánchez, María Paula, Kelly Johanna Lozano-villegas, Iang Schroniltgen Rondón-barragán, and Roy Rodríguez-hernández. "Identification of reference genes for expression studies in the liver, and spleen of laying hens housed in cage and cage-free systems." Open Veterinary Journal 13 (2023), 270-277. doi:10.5455/OVJ.2023.v13.i3.3


MLA (The Modern Language Association) Style

Herrera-sánchez, María Paula, Kelly Johanna Lozano-villegas, Iang Schroniltgen Rondón-barragán, and Roy Rodríguez-hernández. "Identification of reference genes for expression studies in the liver, and spleen of laying hens housed in cage and cage-free systems." Open Veterinary Journal 13.3 (2023), 270-277. Print. doi:10.5455/OVJ.2023.v13.i3.3


APA (American Psychological Association) Style

Herrera-sánchez, M. P., Lozano-villegas, . K. J., Rondón-barragán, . I. S. & Rodríguez-hernández, . R. (2023) Identification of reference genes for expression studies in the liver, and spleen of laying hens housed in cage and cage-free systems. Open Veterinary Journal, 13 (3), 270-277. doi:10.5455/OVJ.2023.v13.i3.3


Author LoginReviewer LoginAbout PublisherPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyTerms of UseLicense InformationCopyright Information

About Open Veterinary Journal

Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Owner, Founder and Editor-in-Chief

Open Veterinary Journal


Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

and


Eldaghayes Publisher

www.eldaghayes.com 

Email: ibrahim.eldaghayes@vetmed.edu.ly