E-ISSN 2218-6050 | ISSN 2226-4485
 

Original Article


Molecular Characterization of Duck Plague Virus from selected Haor (Wetland) areas of Bangladesh

Md. Kamrul Ahmed Khan , Md. Alimul Islam, Abdullah Al Momen Sabuj, Md. Abul Basher, Md. Saiful Islam, Md. Golzar Hossain, Muhammad Toffazzal Hossain, Sukumar Saha.


Cited By:6

Abstract
Background: Duck viral enteritis commonly known as duck plague (DP) is an acute and a contagious fatal disease of duck, geese and swan caused by duck plague virus (DPV). It poses serious threat to growth of duck farming in Haor (Wetland) area of Bangladesh.
Aim: The study aimed to detect circulating duck plague viruses (DPV) by molecular characterization followed by phylogenetic analysis, targeting UL30 gene from infected ducks of five Haor (wetland) districts of Bangladesh and observation of variation in the genome sequence of between the field virus and vaccine strain of DPV.
Methods: A total of 150 samples (liver-50, intestine-50 and oropharyngeal tissue-50) were collected from DP suspected sick/dead ducks of 50 affected farms of Kishoreganj, Netrokona, B-Baria, Habiganj and Sunamganj districts of Bangladesh. For identification of DPV in collected samples, polymerase chain reaction (PCR) was utilized. Nucleotide sequences of amplified UL30 gene were compared to other DPV strains available in GenBank.
Results: In PCR, out of 150 samples, 90 (60%) were found positive for DPV. Organ wise prevalence was higher in liver (72%) followed by intestine and oro-pharyngeal (44%) whereas area wise was from 56.28 to 62.96%. Two of the isolates, BAU/KA/DPV(B1)/2014 from Kishoreganj and BAU/KA/DPV(B4)/2014 from Sunamganj were sequenced and phylogenetic analysis revealed that these isolates are evolutionarily closely related to Chinese isolates of DPV. In addition, the isolates of DPV, BAU/DPV (B1)/2014 and BAU/DPV (B4)/2014 showed the highest (98%) similarity with each other. Nucleotide sequence of the isolate (BAU/KA/DPV (B1)/2014) showed higher variability in nucleotides (264nt.) compared with that of the vaccine strain (Accession no. EU082088), which may impact on protein function and additional drug sensitivity.
Conclusion: From the findings of molecular study, it can be assumed that both Bangladeshi isolates and all the Chinese isolates of DPV may have common ancestry.

Key words: Duck plague virus, PCR, duck embryo, nucleotide sequence


 
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How to Cite this Article
Pubmed Style

MKAK, Islam MA, Sabuj AAM, Basher MA, Islam MS, Hossain MG, Hossain MT, Saha S, . Molecular Characterization of Duck Plague Virus from selected Haor (Wetland) areas of Bangladesh. Open Vet J. 2021; 11(1): 42-51. doi:10.4314/ovj.v11i1.8


Web Style

MKAK, Islam MA, Sabuj AAM, Basher MA, Islam MS, Hossain MG, Hossain MT, Saha S, . Molecular Characterization of Duck Plague Virus from selected Haor (Wetland) areas of Bangladesh. https://www.openveterinaryjournal.com/?mno=129053 [Access: June 04, 2024]. doi:10.4314/ovj.v11i1.8


AMA (American Medical Association) Style

MKAK, Islam MA, Sabuj AAM, Basher MA, Islam MS, Hossain MG, Hossain MT, Saha S, . Molecular Characterization of Duck Plague Virus from selected Haor (Wetland) areas of Bangladesh. Open Vet J. 2021; 11(1): 42-51. doi:10.4314/ovj.v11i1.8



Vancouver/ICMJE Style

MKAK, Islam MA, Sabuj AAM, Basher MA, Islam MS, Hossain MG, Hossain MT, Saha S, . Molecular Characterization of Duck Plague Virus from selected Haor (Wetland) areas of Bangladesh. Open Vet J. (2021), [cited June 04, 2024]; 11(1): 42-51. doi:10.4314/ovj.v11i1.8



Harvard Style

, M. K. A. K., Islam, M. A., Sabuj, A. A. M., Basher, M. A., Islam, M. S., Hossain, M. G., Hossain, M. T., Saha, S. & (2021) Molecular Characterization of Duck Plague Virus from selected Haor (Wetland) areas of Bangladesh. Open Vet J, 11 (1), 42-51. doi:10.4314/ovj.v11i1.8



Turabian Style

, Md. Kamrul Ahmed Khan, Md. Alimul Islam, Abdullah Al Momen Sabuj, Md. Abul Basher, Md. Saiful Islam, Md. Golzar Hossain, Muhammad Toffazzal Hossain, Sukumar Saha, and . 2021. Molecular Characterization of Duck Plague Virus from selected Haor (Wetland) areas of Bangladesh. Open Veterinary Journal, 11 (1), 42-51. doi:10.4314/ovj.v11i1.8



Chicago Style

, Md. Kamrul Ahmed Khan, Md. Alimul Islam, Abdullah Al Momen Sabuj, Md. Abul Basher, Md. Saiful Islam, Md. Golzar Hossain, Muhammad Toffazzal Hossain, Sukumar Saha, and . "Molecular Characterization of Duck Plague Virus from selected Haor (Wetland) areas of Bangladesh." Open Veterinary Journal 11 (2021), 42-51. doi:10.4314/ovj.v11i1.8



MLA (The Modern Language Association) Style

, Md. Kamrul Ahmed Khan, Md. Alimul Islam, Abdullah Al Momen Sabuj, Md. Abul Basher, Md. Saiful Islam, Md. Golzar Hossain, Muhammad Toffazzal Hossain, Sukumar Saha, and . "Molecular Characterization of Duck Plague Virus from selected Haor (Wetland) areas of Bangladesh." Open Veterinary Journal 11.1 (2021), 42-51. Print. doi:10.4314/ovj.v11i1.8



APA (American Psychological Association) Style

, M. K. A. K., Islam, M. A., Sabuj, A. A. M., Basher, M. A., Islam, M. S., Hossain, M. G., Hossain, M. T., Saha, S. & (2021) Molecular Characterization of Duck Plague Virus from selected Haor (Wetland) areas of Bangladesh. Open Veterinary Journal, 11 (1), 42-51. doi:10.4314/ovj.v11i1.8