E-ISSN 2218-6050 | ISSN 2226-4485
 

Original Article


TaqMan probe-based qPCR method for specific detection and quantification of Fowl adenovirus 8b challenge from chickens inoculated with live attenuated or inactivated virus

Chidozie Clifford Ugwu, Mohd Hair Bejo, Abdul Rahman Omar, Nurulfiza Mat Isa, Aini Ideris.


Cited By:2

Abstract
Background:
Fowl adenovirus (FAdV) 8b and other serotypes cause inclusion body hepatitis (IBH) in chickens. Specific detection of aetiologic serotype in mixed infection and vaccine failure could be difficult.
Aim:
The objective of this study was to develop a TaqMan probe-based qPCR method for the detection and quantification of the FAdV 8b challenge virus.
Methods:
Forty-eight broiler chickens inoculated with live attenuated or inactivated FAdV 8b strains at day 1 of age either with or without booster at day 14 post-inoculation were used. The chickens were challenged with a pathogenic strain of FAdV 8b at day 28 of age. Liver and cloacal swabs were collected on days 7 and 14 post-challenge. Primers and probe were designed, specificity confirmed, and used to carry out qPCR amplification.
Results:
The assay amplified the FAdV DNA challenge virus, but not that of the live attenuated virus. It could detect FAdV 8b DNA as low as 0.001ng/µl in liver and cloacal swab samples. Copy numbers obtained indicate virus load and shedding.
Conclusions:
It shows that a selective detection of FAdV 8b within serotype is possible. It can be useful for rapid detection and diagnosis of the disease, virus quantification and differentiation within species, determination of vaccination failure and efficacy especially the virus load in the target organ and shedding.

Key words: Fowl adenovirus 8b, Real time PCR, TaqMan probe, Virus detection, Virus quantification


 
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How to Cite this Article
Pubmed Style

CCU, Bejo MH, Omar AR, Isa NM, Ideris A. TaqMan probe-based qPCR method for specific detection and quantification of Fowl adenovirus 8b challenge from chickens inoculated with live attenuated or inactivated virus. Open Vet J. 2023; 13(2): 171-178. doi:10.5455/OVJ.2023.v13.i2.4


Web Style

CCU, Bejo MH, Omar AR, Isa NM, Ideris A. TaqMan probe-based qPCR method for specific detection and quantification of Fowl adenovirus 8b challenge from chickens inoculated with live attenuated or inactivated virus. https://www.openveterinaryjournal.com/?mno=112776 [Access: June 22, 2024]. doi:10.5455/OVJ.2023.v13.i2.4


AMA (American Medical Association) Style

CCU, Bejo MH, Omar AR, Isa NM, Ideris A. TaqMan probe-based qPCR method for specific detection and quantification of Fowl adenovirus 8b challenge from chickens inoculated with live attenuated or inactivated virus. Open Vet J. 2023; 13(2): 171-178. doi:10.5455/OVJ.2023.v13.i2.4



Vancouver/ICMJE Style

CCU, Bejo MH, Omar AR, Isa NM, Ideris A. TaqMan probe-based qPCR method for specific detection and quantification of Fowl adenovirus 8b challenge from chickens inoculated with live attenuated or inactivated virus. Open Vet J. (2023), [cited June 22, 2024]; 13(2): 171-178. doi:10.5455/OVJ.2023.v13.i2.4



Harvard Style

, C. C. U., Bejo, . M. H., Omar, . A. R., Isa, . N. M. & Ideris, . A. (2023) TaqMan probe-based qPCR method for specific detection and quantification of Fowl adenovirus 8b challenge from chickens inoculated with live attenuated or inactivated virus. Open Vet J, 13 (2), 171-178. doi:10.5455/OVJ.2023.v13.i2.4



Turabian Style

, Chidozie Clifford Ugwu, Mohd Hair Bejo, Abdul Rahman Omar, Nurulfiza Mat Isa, and Aini Ideris. 2023. TaqMan probe-based qPCR method for specific detection and quantification of Fowl adenovirus 8b challenge from chickens inoculated with live attenuated or inactivated virus. Open Veterinary Journal, 13 (2), 171-178. doi:10.5455/OVJ.2023.v13.i2.4



Chicago Style

, Chidozie Clifford Ugwu, Mohd Hair Bejo, Abdul Rahman Omar, Nurulfiza Mat Isa, and Aini Ideris. "TaqMan probe-based qPCR method for specific detection and quantification of Fowl adenovirus 8b challenge from chickens inoculated with live attenuated or inactivated virus." Open Veterinary Journal 13 (2023), 171-178. doi:10.5455/OVJ.2023.v13.i2.4



MLA (The Modern Language Association) Style

, Chidozie Clifford Ugwu, Mohd Hair Bejo, Abdul Rahman Omar, Nurulfiza Mat Isa, and Aini Ideris. "TaqMan probe-based qPCR method for specific detection and quantification of Fowl adenovirus 8b challenge from chickens inoculated with live attenuated or inactivated virus." Open Veterinary Journal 13.2 (2023), 171-178. Print. doi:10.5455/OVJ.2023.v13.i2.4



APA (American Psychological Association) Style

, C. C. U., Bejo, . M. H., Omar, . A. R., Isa, . N. M. & Ideris, . A. (2023) TaqMan probe-based qPCR method for specific detection and quantification of Fowl adenovirus 8b challenge from chickens inoculated with live attenuated or inactivated virus. Open Veterinary Journal, 13 (2), 171-178. doi:10.5455/OVJ.2023.v13.i2.4