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2023, Vol: 13, Issue: 8
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Fluorescent multiple staining and CASA system to assess boar sperm viability and membranes integrity in short and long-term extenders

A. Lange-Consiglio, A. Meucci and F. Cremonesi*.

Abstract
The aim of this study was to assess the effect on boar spermatozoa quality of in vitro storage in short and long-term extenders by fluorescent multiple staining (FMS) and computer assisted semen analyzer (CASA). Fresh ejaculates from three healthy, sexually mature boars were diluted with equal volumes of six short-term or three long-term commercial extenders and stored at 19°C for 6 days (short-term) or 12 days (long-term). The integrity of spermatozoa membranes was analyzed by FMS using propidium iodide, 5,5’,6,6’-tetrachloro-1,1’,3,3’ tetraethylbenzimidazolyl-carbocyanine iodide (JC-1) and fluorescein isothiocyanate-conjugated peanut agglutinin (PNA). The results obtained from this staining were compared with spermatozoa motility assessed by CASA. Our study showed that the number of viable spermatozoa with non-reacted acrosomes and intact mitochondria was positively correlated with the rate of motile spermatozoa (r2>0.9) irrespective of the extender used. In all extenders the number of motile spermatozoa significantly decreased as preservation period increased (P<0.05). FMS test is a potent indicator of sperm motility because it analyses mitochondrial integrity independently from observable alterations in motility. The best performing extenders were BTS for short-term storage and TRI-x-Cell for long-term storage.

Key words: Boar, CASA, Fluorescent staining, Motility, Semen storage.


 
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Pubmed Style

A. Lange-Consiglio, A. Meucci and F. Cremonesi. Fluorescent multiple staining and CASA system to assess boar sperm viability and membranes integrity in short and long-term extenders. Open Vet J. 2013; 3(1): 21-35. doi:10.5455/OVJ.2013.v3.i1.p21

Web Style

A. Lange-Consiglio, A. Meucci and F. Cremonesi. Fluorescent multiple staining and CASA system to assess boar sperm viability and membranes integrity in short and long-term extenders. https://www.openveterinaryjournal.com/?mno=302656871 [Access: September 12, 2023]. doi:10.5455/OVJ.2013.v3.i1.p21

AMA (American Medical Association) Style

A. Lange-Consiglio, A. Meucci and F. Cremonesi. Fluorescent multiple staining and CASA system to assess boar sperm viability and membranes integrity in short and long-term extenders. Open Vet J. 2013; 3(1): 21-35. doi:10.5455/OVJ.2013.v3.i1.p21


Vancouver/ICMJE Style

A. Lange-Consiglio, A. Meucci and F. Cremonesi. Fluorescent multiple staining and CASA system to assess boar sperm viability and membranes integrity in short and long-term extenders. Open Vet J. (2013), [cited September 12, 2023]; 3(1): 21-35. doi:10.5455/OVJ.2013.v3.i1.p21


Harvard Style

A. Lange-Consiglio, A. Meucci and F. Cremonesi (2013) Fluorescent multiple staining and CASA system to assess boar sperm viability and membranes integrity in short and long-term extenders. Open Vet J, 3 (1), 21-35. doi:10.5455/OVJ.2013.v3.i1.p21


Turabian Style

A. Lange-Consiglio, A. Meucci and F. Cremonesi. 2013. Fluorescent multiple staining and CASA system to assess boar sperm viability and membranes integrity in short and long-term extenders. Open Veterinary Journal, 3 (1), 21-35. doi:10.5455/OVJ.2013.v3.i1.p21


Chicago Style

A. Lange-Consiglio, A. Meucci and F. Cremonesi. "Fluorescent multiple staining and CASA system to assess boar sperm viability and membranes integrity in short and long-term extenders." Open Veterinary Journal 3 (2013), 21-35. doi:10.5455/OVJ.2013.v3.i1.p21


MLA (The Modern Language Association) Style

A. Lange-Consiglio, A. Meucci and F. Cremonesi. "Fluorescent multiple staining and CASA system to assess boar sperm viability and membranes integrity in short and long-term extenders." Open Veterinary Journal 3.1 (2013), 21-35. Print. doi:10.5455/OVJ.2013.v3.i1.p21


APA (American Psychological Association) Style

A. Lange-Consiglio, A. Meucci and F. Cremonesi (2013) Fluorescent multiple staining and CASA system to assess boar sperm viability and membranes integrity in short and long-term extenders. Open Veterinary Journal, 3 (1), 21-35. doi:10.5455/OVJ.2013.v3.i1.p21


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