Open Veterinary Journal

Open Veterinary Journal

Peer-Reviewed Journal 
ISSN 2218-6050 (Online), ISSN 2226-4485 (Print) 

"Original Research"

Application of gene therapy in the treatment of superficial digital flexor tendon injury in horses


Alexandr Aimaletdinov(1), Gulnur Mindubaeva(2), Svetlana Khalikova(2), Emmanuel Kabwe(1,3), Alexandra Salmakova(1), Natalia Alexandrova(1), Catrin Rutland(4), 

Albert Rizvanov(1) and Elena Zakirova(1*)


1- Department of Exploratory Research, Scientific and Educational Center of Pharmaceutics, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russian Federation

2- International equestrian center «Kazan», Russian Federation

3- Kazan Research Institute of Epidemiology and Microbiology, Kazan, the Republic of Tatarstan, Russian Federation

4- Faculty of Medicine, School of Veterinary Medicine and Science, University of Nottingham, Nottingham, United Kingdom

Abstract

Background: Tendon injuries are one of the most common causes of orthopedic disorders in horses. Such injuries involve a long course of treatment and recovery. The most promising method of treating these injuries is the use of recombinant proteins and gene therapy.

Aim: In this work, we evaluated the therapeutic efficacy of plasmid DNA containing two species-specific coding sequences of vascular endothelial growth factor 164 and fibroblast growth factor 2 in the treatment of severe damage to the tendon of the superficial digital flexor.

Methods: A plasmid DNA construct was used to restore the damaged superficial digital flexor tendon in the horse.

Results: The study showed that administration of plasmid DNA encoding vascular endothelial growth factor 164 and fibroblast growth factor 2 genes at the injury area increased the regenerative activities of the damaged tendon.

Conclusion: This study shows the therapeutic properties of genetic constructs (pDNA) and contributes to the advancements in the use of these therapies.

Keywords: Fibroblast growth factor 2, Plasmid DNA, Superficial digital flexor, Vascular endothelial growth factor.

Cite this paper:

Aimaletdinov, A., Mindubaeva, G., Khalikova, S., Kabwe, E., Salmakova, A., Alexandrova, N., Rutland, C., Rizvanov, A. and Zakirova, E. 2020. Application of gene therapy in the treatment of superficial digital flexor tendon injury in horses. Open Vet. J. 10(3), 261-266.