Alexandr Aimaletdinov(1), Gulnur Mindubaeva(2), Svetlana Khalikova(2), Emmanuel Kabwe(1,3), Alexandra Salmakova(1), Natalia Alexandrova(1), Catrin Rutland(4),
Albert Rizvanov(1) and Elena Zakirova(1*)
1- Department of Exploratory Research, Scientific and Educational Center of Pharmaceutics, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russian Federation
2- International equestrian center «Kazan», Russian Federation
3- Kazan Research Institute of Epidemiology and Microbiology, Kazan, the Republic of Tatarstan, Russian Federation
4- Faculty of Medicine, School of Veterinary Medicine and Science, University of Nottingham, Nottingham, United Kingdom
Background: Tendon injuries are one of the most common causes of orthopedic disorders in horses. Such injuries involve a long course of treatment and recovery. The most promising method of treating these injuries is the use of recombinant proteins and gene therapy.
Aim: In this work, we evaluated the therapeutic efficacy of plasmid DNA containing two species-specific coding sequences of vascular endothelial growth factor 164 and fibroblast growth factor 2 in the treatment of severe damage to the tendon of the superficial digital flexor.
Methods: A plasmid DNA construct was used to restore the damaged superficial digital flexor tendon in the horse.
Results: The study showed that administration of plasmid DNA encoding vascular endothelial growth factor 164 and fibroblast growth factor 2 genes at the injury area increased the regenerative activities of the damaged tendon.
Conclusion: This study shows the therapeutic properties of genetic constructs (pDNA) and contributes to the advancements in the use of these therapies.
Keywords: Fibroblast growth factor 2, Plasmid DNA, Superficial digital flexor, Vascular endothelial growth factor.
Cite this paper:
Aimaletdinov, A., Mindubaeva, G., Khalikova, S., Kabwe, E., Salmakova, A., Alexandrova, N., Rutland, C., Rizvanov, A. and Zakirova, E. 2020. Application of gene therapy in the treatment of superficial digital
flexor tendon injury in horses. Open Vet. J. 10(3), 261-266.