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Research Article


Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hosts

Theerayut Thongrin, Watcharapol Suyapoh, Woro Danur Wendo, Prasarn Tangkawattana, Peerapol Sukon, Kanin Salao, Sutas Suttiprapa, Prasert Saichua, Sirikachorn Tangkawatana.


Cited By:1

Abstract
Background:
Individual host susceptibility is believed to be a risk factor in the interaction between the host and the parasite. Since studying time series in humans is limited, animal models are replaced.
Aim:
This study aims to explore and compare the pattern of inflammatory cell types along the biliary tract and their association with proliferative lesions in the early development of CCA from susceptible and non-susceptible animal models.
Methods:
Thirty male Syrian golden hamsters and 30 BALB/c mice, serving as the susceptible and non-susceptible animal models, were used in this comparative study. The animals were infected with 50 O. viverrini metacercariae via gastric intubation. At days 1, 2, 7, 14, 28, and 56 post-infection (p.i.), five animals were randomly selected from each group and humanely sacrificed. The hepatobiliary tissues were collected and processed for histopathological study. Histochemical and immunohistochemical staining were applied to differentiate the inflammatory cell types. Kruskal-Wallis and Mann-Whitney tests were applied to assess all semi-quantitative and quantitative variables. The correlation between each variable was also analyzed using Spearman rank at a p-value <0.05.
Results:
The results demonstrated that mice had different patterns of infiltrating cell types when compared to hamsters. This suggested that the cellular response to the infection in mice occurred earlier than that in hamsters. The response in mice reached its peak at D7 to D14 and then rapidly declined at D28. In contrast, although the inflammatory response in hamsters started slowly, the response reached the peak at D28 and maintained a high level until D56. Significant differences in the number of inflammatory cells between mice and hamsters were seen at D1 (p=0.047), D7 (p=0.049), D28 (p=0.040), and D56 (p<0.040).
Conclusion:
The inflammatory responses to O. viverrini infection in the non-susceptible animal model occurred and declined earlier while the response in the susceptible animal model occurred later in a gradual manner. Both rodents are suitable animal models for the studies of opisthorchiasis susceptibility.

Key words: Inflammatory cells, Biliary, Proliferation, Opisthorchis viverrini, Susceptible and non-susceptible host


 
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How to Cite this Article
Pubmed Style

Thongrin T, Suyapoh W, Wendo WD, Tangkawattana P, Sukon P, Salao K, Suttiprapa S, Saichua P, Tangkawatana S. Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hosts. Open Vet J. 2023; 13(9): 1150-1166. doi:10.5455/OVJ.2023.v13.i9.11

Web Style

Thongrin T, Suyapoh W, Wendo WD, Tangkawattana P, Sukon P, Salao K, Suttiprapa S, Saichua P, Tangkawatana S. Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hosts. https://www.openveterinaryjournal.com/?mno=159603 [Access: May 02, 2024]. doi:10.5455/OVJ.2023.v13.i9.11

AMA (American Medical Association) Style

Thongrin T, Suyapoh W, Wendo WD, Tangkawattana P, Sukon P, Salao K, Suttiprapa S, Saichua P, Tangkawatana S. Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hosts. Open Vet J. 2023; 13(9): 1150-1166. doi:10.5455/OVJ.2023.v13.i9.11


Vancouver/ICMJE Style

Thongrin T, Suyapoh W, Wendo WD, Tangkawattana P, Sukon P, Salao K, Suttiprapa S, Saichua P, Tangkawatana S. Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hosts. Open Vet J. (2023), [cited May 02, 2024]; 13(9): 1150-1166. doi:10.5455/OVJ.2023.v13.i9.11


Harvard Style

Thongrin, T., Suyapoh, . W., Wendo, . W. D., Tangkawattana, . P., Sukon, . P., Salao, . K., Suttiprapa, . S., Saichua, . P. & Tangkawatana, . S. (2023) Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hosts. Open Vet J, 13 (9), 1150-1166. doi:10.5455/OVJ.2023.v13.i9.11


Turabian Style

Thongrin, Theerayut, Watcharapol Suyapoh, Woro Danur Wendo, Prasarn Tangkawattana, Peerapol Sukon, Kanin Salao, Sutas Suttiprapa, Prasert Saichua, and Sirikachorn Tangkawatana. 2023. Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hosts. Open Veterinary Journal, 13 (9), 1150-1166. doi:10.5455/OVJ.2023.v13.i9.11


Chicago Style

Thongrin, Theerayut, Watcharapol Suyapoh, Woro Danur Wendo, Prasarn Tangkawattana, Peerapol Sukon, Kanin Salao, Sutas Suttiprapa, Prasert Saichua, and Sirikachorn Tangkawatana. "Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hosts." Open Veterinary Journal 13 (2023), 1150-1166. doi:10.5455/OVJ.2023.v13.i9.11


MLA (The Modern Language Association) Style

Thongrin, Theerayut, Watcharapol Suyapoh, Woro Danur Wendo, Prasarn Tangkawattana, Peerapol Sukon, Kanin Salao, Sutas Suttiprapa, Prasert Saichua, and Sirikachorn Tangkawatana. "Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hosts." Open Veterinary Journal 13.9 (2023), 1150-1166. Print. doi:10.5455/OVJ.2023.v13.i9.11


APA (American Psychological Association) Style

Thongrin, T., Suyapoh, . W., Wendo, . W. D., Tangkawattana, . P., Sukon, . P., Salao, . K., Suttiprapa, . S., Saichua, . P. & Tangkawatana, . S. (2023) Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hosts. Open Veterinary Journal, 13 (9), 1150-1166. doi:10.5455/OVJ.2023.v13.i9.11


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