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Research Article


Specular microscopy of the corneal endothelial cells of bovines: An ex vivo study

Mariane Gallicchio Azevedo, Natália Pons Méndez, Luísa Soares Cargnin, Rafaella Silva Rocha, Maiara Poersch Seibel, Alessandra Fernandez da Silva, João Antonio Tadeu Pigatto.


Cited By:3

Abstract
Background:
The endothelium is the most posterior layer of the cornea and essential for maintaining corneal transparency. Due to variations in corneal endothelial parameters among different species, knowledge of the normal parameters for each species is crucial.
Aim:
To evaluate the corneal endothelium of bovines using contact specular microscopy.
Methods:
Twenty eyeballs from 10 male Brangus (Bos taurus) aged 24 months were evaluated. Contact specular microscopy was performed on the central corneal area. The analysed parameters were endothelial cell density (ECD) and endothelial cell morphology.
Results:
The ECD in the central area was 1277 cells/mm². Regarding the morphology, mainly cells with six (74.3%), five (14.7%) and seven sides (10%) were found. There were no significant differences in endothelial cell density and morphology between left and right eyes.
Conclusion:
Contact specular microscopy facilitated the analysis and measurement of corneal endothelial parameters in bovines. The data obtained will serve as a reference for the analysis of bovine corneal endothelium.

Key words: Cornea, Endothelium, Morphology, Cattle


 
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How to Cite this Article
Pubmed Style

Azevedo MG, Méndez NP, Cargnin LS, Rocha RS, Seibel MP, Silva AFd, Pigatto JAT. Specular microscopy of the corneal endothelial cells of bovines: An ex vivo study. Open Vet J. 2023; 13(12): 1554-1561. doi:10.5455/OVJ.2023.v13.i12.5

Web Style

Azevedo MG, Méndez NP, Cargnin LS, Rocha RS, Seibel MP, Silva AFd, Pigatto JAT. Specular microscopy of the corneal endothelial cells of bovines: An ex vivo study. https://www.openveterinaryjournal.com/?mno=153541 [Access: May 02, 2024]. doi:10.5455/OVJ.2023.v13.i12.5

AMA (American Medical Association) Style

Azevedo MG, Méndez NP, Cargnin LS, Rocha RS, Seibel MP, Silva AFd, Pigatto JAT. Specular microscopy of the corneal endothelial cells of bovines: An ex vivo study. Open Vet J. 2023; 13(12): 1554-1561. doi:10.5455/OVJ.2023.v13.i12.5


Vancouver/ICMJE Style

Azevedo MG, Méndez NP, Cargnin LS, Rocha RS, Seibel MP, Silva AFd, Pigatto JAT. Specular microscopy of the corneal endothelial cells of bovines: An ex vivo study. Open Vet J. (2023), [cited May 02, 2024]; 13(12): 1554-1561. doi:10.5455/OVJ.2023.v13.i12.5


Harvard Style

Azevedo, M. G., Méndez, . N. P., Cargnin, . L. S., Rocha, . R. S., Seibel, . M. P., Silva, . A. F. d. & Pigatto, . J. A. T. (2023) Specular microscopy of the corneal endothelial cells of bovines: An ex vivo study. Open Vet J, 13 (12), 1554-1561. doi:10.5455/OVJ.2023.v13.i12.5


Turabian Style

Azevedo, Mariane Gallicchio, Natália Pons Méndez, Luísa Soares Cargnin, Rafaella Silva Rocha, Maiara Poersch Seibel, Alessandra Fernandez da Silva, and João Antonio Tadeu Pigatto. 2023. Specular microscopy of the corneal endothelial cells of bovines: An ex vivo study. Open Veterinary Journal, 13 (12), 1554-1561. doi:10.5455/OVJ.2023.v13.i12.5


Chicago Style

Azevedo, Mariane Gallicchio, Natália Pons Méndez, Luísa Soares Cargnin, Rafaella Silva Rocha, Maiara Poersch Seibel, Alessandra Fernandez da Silva, and João Antonio Tadeu Pigatto. "Specular microscopy of the corneal endothelial cells of bovines: An ex vivo study." Open Veterinary Journal 13 (2023), 1554-1561. doi:10.5455/OVJ.2023.v13.i12.5


MLA (The Modern Language Association) Style

Azevedo, Mariane Gallicchio, Natália Pons Méndez, Luísa Soares Cargnin, Rafaella Silva Rocha, Maiara Poersch Seibel, Alessandra Fernandez da Silva, and João Antonio Tadeu Pigatto. "Specular microscopy of the corneal endothelial cells of bovines: An ex vivo study." Open Veterinary Journal 13.12 (2023), 1554-1561. Print. doi:10.5455/OVJ.2023.v13.i12.5


APA (American Psychological Association) Style

Azevedo, M. G., Méndez, . N. P., Cargnin, . L. S., Rocha, . R. S., Seibel, . M. P., Silva, . A. F. d. & Pigatto, . J. A. T. (2023) Specular microscopy of the corneal endothelial cells of bovines: An ex vivo study. Open Veterinary Journal, 13 (12), 1554-1561. doi:10.5455/OVJ.2023.v13.i12.5


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