Achmad Firdaus Sani(1,2), Widjiati Widjiati(3*), Paulus Sugianto(2), Muhammad Hamdan(2) and Jovian Philip Swatan(2)
1- Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Indonesia
2- Department of Neurology, Faculty of Medicine, Universitas Airlangga /Dr. Soetomo General Hospital, Jl. Mayjen Prof. Dr. Moestopo No.47, Surabaya 60132 Indonesia
3- Department of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga Jl Muyorejo Surabaya, 60115 Indonesia
Background: Asymptomatic carotid artery stenosis has become more prevalent worldwide and is often associated with a poor prognosis. Numerous guidelines highlighted surgical interventions as treatment for carotid artery stenosis, but only a few recommendations were made regarding non-surgical interventions due to its limited data.
Aims: This study aims to develop a mice model for research in non-surgical interventions of asymptomatic carotid artery stenosis.
Methods: Adult male Rattus Norvegicus, Wistar strain models with bilateral asymptomatic common carotid artery stenosis (BACAS) were created by ligating the common carotid artery with a 0.6 mm diameter needle and then removing the needle. The mice’s body weight, clinical signs and symptoms, and post-mortem brain analysis were compared between the sham-operated group and the BACAS group.
Results: The mortality rate among BACAS group is 11.11%. There is no significant difference in mean body weight before surgery, after the observation period, and percentage of weight decrease between sham-operated and BACAS groups (p=0.710, 0.632, and 0.806, respectively). None of the surviving mice in this study exhibit signs of motor paralysis. Gross examination of the brain reveals no signs of infarction or hemorrhage.
Conclusion: We have established a novel BACAS mouse model which is cost-efficient, easy to produce, and with no significant alteration in body weight, clinical parameters, and brain morphology.
Keywords: Animal models of disease, Carotid arteries, Mouse models, Non-communicable disease, Scientific research.
Cite this paper:
Sani, A.F., Widjiati, W., Sugianto, P., Hamdan, M. and Swatan, J.P. 2022. Bilateral asymptomatic common carotid artery stenosis (BACAS): Mouse
model for stroke research. Open Vet. J. 12(4), 463-468.