Open Veterinary Journal

Open Veterinary Journal

Peer-Reviewed Journal 
ISSN 2218-6050 (Online), ISSN 2226-4485 (Print) 


Collection, analysis and cryopreservation of semen from Malayan gaur (Bos gaurus hubbacki): A preliminary study 

M.I. Iswadi1, Z.F. Ann1, M.M. Hafiz2, M.D. Hafiz2, F.J. Fahrul2, H. Hajarian3, H. Wahid3, I. Zawawi4, M.S. Khairiah5 and O.A. Mazni6

1Veterinary Research Institute (VRI), Department of Veterinary Services, 59, Jalan Sultan Azlan Shah, 31400 Ipoh, Perak, Malaysia

2Agro-Biotechnology Institute, Malaysia (ABI), Ministry of Science, Technology and Innovation, P.O Box 341, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

3Veterinary Hospital, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

4National Institute of Veterinary Biodiversity, Department of Veterinary Services, Bukit Dinding, 27000 Jerantut, Pahang, Malaysia

5Department Of Wildlife and National Parks, Pahang, Bangunan Asia Life, Jalan Teluk Sisek, 25000 Kuantan, Pahang, Malaysia

6Malaysian Agricultural Research and Development Institute (MARDI), 43400 Serdang, Selangor, Malaysia



The Malayan gaur (Bos gaurus hubbacki) or Seladang is classified as vulnerable by the International Union for Conservation of Nature and Natural Resources (IUCN). The Malayan gaur is mainly distributed in the tropical woodlands of Peninsular Malaysia and Southern Thailand. The aim of this study was to collect, analyze and cryopreserve the semen of wild Malayan gaur. Transrectal massage (TM) and electroejaculation (EEJ) technique was applied in semen collection of the Malayan gaur. The semen was then cryopreserved in liquid nitrogen using slow freezing technique. Makler counting chamber was used to evaluate sperm concentration and motility, while the sperm viability and morphology of fresh and post-thaw sperm was determined using eosin-nigrosin staining protocol. As a result, we have successfully collected the Malayan gaur semen using EEJ technique. Sperm motility, viability and morphological changes of the post-thaw semen of Malayan gaur were found undesirable due to the complication of the cryopreservation process. On the basis of current study it can be concluded that Malayan gaur bulls semen can be obtain by EEJ with no evidence of rectal trauma. Optimization of the process of cryopreservation for Malayan gaur sperm is needed to maintain the cryoviability of the good sperm quality. The data generated in this study would be useful in conservation of genetic diversity program for Malayan gaur.

Key words: Cryopreservation, Malayan gaur, Semen collection.


Cite this paper:

Iswadi, M.I., Ann, Z.F., Hafiz, M.M., Hafiz, M.D., Fahrul, F.J., Hajarian, H., Wahid, H., Zawawi, I., Khairiah, M.S. and Mazni, O.A. 2012. Collection, analysis and cryopreservation of semen from Malayan gaur (Bos gaurus hubbacki): A preliminary study. Open Vet. J. 2, 109-114.