Open Veterinary Journal

Peer-Reviewed Journal 
ISSN 2218-6050 (Online), ISSN 2226-4485 (Print) 


Influence of different storage times and temperatures on blood gas and acid-base balance in ovine venous blood

H.A. Hussein* and A.A. Aamer

Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt



The present study was designed to investigate the effects of storage temperature and time on blood gas and acid-base balance of ovine venous blood. Ten clinically healthy sheep were used in this study. A total number of 30 blood samples, were divided into three different groups, and were stored in a refrigerator adjusted to +4 ºC (Group I,          n = 10), at RT of about 22-25 ºC (Group II, n = 10) and in an incubator adjusted to 37 ºC (Group III, n = 10) for up to 48 h. Blood samples were analysed for blood gas and acid-base indices at 0, 1, 2, 3, 4, 5, 6, 12, 24 and 48 h of storage. In comparison to the baseline value (0), there were significant decreases of blood pH of samples stored at RT and in the incubator after 1 h (p<0.05), the pH value of refrigerated blood samples exhibited insignificant changes during the study (p<0.05). Mean values of pCO2 showed a significant increase in Group I and Group III after 1 h then a progressive decrease after 12 h in all Groups. Mean pO2 values were significantly higher for Group I after 2 h and for Groups II and III after 1 h (p<0.05). In general, base excess decreased significantly for all the groups during the study especially in Groups II and III. In comparison with baseline values, in all groups, bicarbonate (HCO3) increased between 1 h and 6 h (p<0.05), and later decreased at the end of the study (p<0.05). In conclusion, status of acid-base indices of the samples stored at refrigerator and RT were found within normal reference range and it may be of clinical diagnostic use for up to 6 h.

Key words: Acid-base indices, Clinical relevance, Ovine, Storage temperature time.


Cite this paper:

Hussein, H.A. and Aamer, A.A. 2013. Influence of different storage times and temperatures on blood gas and acid-base balance in ovine venous blood. Open Vet. J. 3(1), 1-7.