Open Veterinary Journal

Open Veterinary Journal

Peer-Reviewed Journal 
ISSN 2218-6050 (Online), ISSN 2226-4485 (Print) 

"Short Communication"

Isolation and preservation of multipotent mesenchymal stem cells from bone marrow of Arabian leopard (Panthera pradus nimr)


Andrzej Golachowski(1,*), Sulaiman Al Hashmi(2) and Barbara Golachowska(1)


Directorate General of Veterinary Services, Royal Court Affairs, PC111 PO 64 Seeb, Oman

Biomedical Laboratory, University of Nizwa P. O. Box : 33, PC 616, Birkat Al-Mouz, Nizwa, Oman

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Abstract

The Arabian leopard (Panthera pardus nimr) is critically endangered by the International Union for Conservation of Nature, with an effective population of 150-250 across its entire range in the Arabian Peninsula. Isolation and preservation of multipotent mesenchymal stem cells is beneficial both for medical and research purposes. The optimal protocol for collection, handling, culturing and preserving the Arabian leopard mesenchymal stem cells acquired from bone marrow was established. Anesthesia with combination of medetomidine and tiletamine-zozalepam is the safest option even for old animals with concurrent disease including chronic kidney disease.

Keywords: Anesthesia, Arabian leopard, Bone marrow, Mesenchymal stem cells, Panthera pardus nimr.

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Cite this paper:

Golachowski, A., Al Hashmi, S. and Golachowska, B. 2018. Isolation and preservation of multipotent mesenchymal stem cells from bone marrow of Arabian leopard (Panthera pradus nimr). Open Vet. J. 8(3), 325-329.