1- Department of Microbiology, Faculty of Veterinary Sciences, The University of Aljfara, Aljfara, Libya
2- Higher Institute of Medical Technology, Department of Microbiology, Abo-Slim, Tripoli, Libya
3- Faculty of Veterinary Medicine, University of Tripoli, P.O. Box 13662, Tripoli, Libya
§ Contributed equally in this study
Background: Newcastle disease (VD) is a viral disease that affecting many avian species all over the world.
Aim: ND has been successfully controlled by vaccination of commercial poultry in Libya. However, there was a lack of information about the situation of ND in backyard chickens. Therefore this study determined the prevalence of ND in backyard chickens in different locations of Tripoli.
Methods: A total number of 280 cloacal swabs (190 in summer and 90 in winter) and 412 sera were collected from non-vaccinated backyard chicken flocks in different geographical locations within the area of Tripoli namely Qasr Ben Ghashier, Al-Sawani, Souq Al-Gomaa, Tajourah, Ein Zara, and Janzour. Cloacal swabs and sera were tested by real time PCR and ELISA, respectively.
Results: The prevalence of NDV infection in backyard chickens in different locations of Tripoli during summer and winter was 45% using rRT-PCR. Except in Qasr Ben Ghashier, the prevalence in summer season was significantly higher than in winter (X2=46.13, P³0.00001). ELISA test revealed 218 positive out of 412 tested samples with total prevalence of 53% across the city of Tripoli in all regions. Obviously, Qasr Ben Ghashier had significantly (X2=74.09, P³0.00001) the highest prevalence (82%) of NDV specific antibodies followed by Tajourah (68%).
Conclusion: This study demonstrated the situation of ND in backyard chicken highlighting the necessity of a comprehensive vaccination plan for backyard chickens.
Keywords: Backyard chickens, ELISA, Newcastle disease, Prevalence, Real time PCR.
Cite this paper:
Gedara, E.A., Alseed, H.A., Kammon, A.M., Dayhum, A.S., Benothman, M.A., Al-Garib, S.O. and Eldaghayes, I.M. 2020. Seroprevalence
and molecular detection of Newcastle disease virus in backyard chickens in
Tripoli, Libya. Open Vet. J. 10(1), 80-85.