Open Veterinary Journal

Open Veterinary Journal

Peer-Reviewed Journal 
ISSN 2218-6050 (Online), ISSN 2226-4485 (Print) 

"Original Research"

Oxidative stress in Strongylus spp. infected donkeys treated with piperazine citrate versus doramectin


Enas Elmeligy(1), Abdelbaset Abdelbaset(2), Hanan K. Elsayed(3), Sara A. Bayomi(2), Ahmed Hafez(4), Ashraf M. Abu-Seida(5), Khaled A.S. El-Khabaz(6), Dalia Hassan(7), Rehab A. Ghandour(8) and Arafat Khalphallah(3*)


1- Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt

2- Division of Clinical Laboratory Diagnosis, Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt

3- Division of Internal Medicine, Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt

4- Department of Pharmacology, Faculty of Veterinary Medicine, Aswan University, Aswan 81528, Egypt

5- Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Cairo University, P.O. Box 12211, Giza, Egypt

6- Division of infectious diseases, Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt

7- Department of Animal & Poultry Hygiene and Environmental Sanitation, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt

8- Department of physiology, Faculty of Veterinary Medicine, Cairo University, P.O. Box 12211, Giza, Egypt

Abstract

Background: Parasitic infection is one of the main problems in equidae, particularly donkeys.

Aim: This study evaluated the oxidative stress in donkeys infected with Strongylus spp by determining the correlation between antioxidants levels; malondialdehyde (MDA), total antioxidant capacity (TAC), and the severity of parasitic infection. It also compared the therapeutic efficacy of piperazine citrate as an oral anthelmintic drug and Doramectin as an injectable one.

Methods: The study was conducted on 40 donkeys naturally infected with Strongylus spp. These donkeys were divided into two groups (20 donkeys each) according to treatment; One group was treated with piperazine citrate [PipTG] and the other with doramectin [DoraTG]. Thorough clinical examination, hematological, biochemical, and parasitological assays were performed before (Day 0) and after treatment (Days 7, 14, 21, and 28). All data were statistically analyzed by independent-sample t-test or paired t-test.

Results: In both groups, mean values of MDA were significantly reduced while those of TAC were significantly elevated after treatment on days 7, 14, 21, and 28. These significant changes were reported after treatment between PipTG and DoraTG in favor of DoraTG. Serum concentrations of MDA were significantly reduced while those of TAC were significantly elevated for DoraTG treatment group when their values were compared with those of PipTG either on days 7, 14, 21, or 28. Significant correlations were reported in PipTG and DoraTG. Negative significant correlations were reported between faecal egg count (FEC) and each of whole blood picture indices (RBCS, Hb and PCV), serum TAC and faecal egg count reduction percentage (FECR%). A positive correlation was seen between FEC and MDA. MDA exhibited a negative correlation with both blood picture and TAC; hence, TAC was positively correlated with these blood picture indices in both PipTG and DoraTG. In PipTG, anthelmintic resistance (R) was present on days 7 and 14, while it was suspected (S) at day 21 then it was absent (N) at day 28. In DoraTG, anthelmintic resistance was suspected (S) on day 7, then it became absent (N) on days 14, 21, and 28 post therapy.

Conclusion: the immunological status of the infected donkeys had greatly improved after treatment. The therapeutic efficacy of injectable doramectin was more efficient than that of oral piperazine citrate in Strongylus spp. infected donkeys.

Keywords: Donkeys, Doramectin, Malondialdehyde, Piperazine citrate, Strongylosis.

Cite this paper:
Elmeligy, E., Abdelbaset, A., Elsayed, H.K., Bayomi, S.A., Hafez, A., Abu-Seida, A.M., El-Khabaz, K.A.S., Hassan, D., Ghandour, R.A. and Khalphallah, A. 2021. Oxidative stress in Strongylus spp. infected donkeys treated with piperazine citrate versus doramectin. Open Vet. J. 11(2), 238-250.