Hannes Beims(1,2*), Martina Janke(1), Werner von der Ohe(1) and Michael Steinert(2)
1- Lower Saxony State Office for Consumer Protection and Food Safety, Institute of Apiculture, Herzogin-Eleonore-Allee 5, 29221 Celle, Germany
2- Institut für Mikrobiologie, Technische Universität Braunschweig, Spielmannstraße 7, 38106 Braunschweig, Germany
Background: American Foulbrood (AFB) is a devastating disease of honey bee (Apis mellifera) larvae caused by the spore-forming, Gram-positive bacterium Paenibacillus larvae. In most countries, the law requires mandatory reporting of AFB to the veterinary authority.
Aim and Methods: To speed up detection and genotyping of P. larvae spores, we compared different culturing protocols on Columbia sheep blood agar (CSA) and developed a new multiplex qPCR to distinguish between the two relevant P. larvae genotypes ERIC I and ERIC II.
Results and Conclusion: As confirmed by P. larvae reference strains and field isolates, the new identification and genotyping protocol halves the time of current workflows, lessens labour-intension, allows a higher throughput of samples for monitoring, and permits a faster intervention to prevent the spread of AFB.
Keywords: American Foulbrood, ERIC genotyping, Multiplex quantitative PCR, Paenibacillus larvae, Rapid detection.
Cite this paper:
Beims, H., Janke, M., von der Ohe, W. and Steinert, M. 2020. Rapid identification and genotyping of the
honeybee pathogen Paenibacillus larvae
by combining culturing and multiplex quantitative PCR. Open Vet. J. 10(1), 53-58.