Open Veterinary Journal

Open Veterinary Journal

Peer-Reviewed Journal 
ISSN 2218-6050 (Online), ISSN 2226-4485 (Print) 

"Short Communication"

Concentration and motility of spermatozoa and testosterone level of kacang goat after seminal vesicle extract administration


Teuku Armansyah(1), Edi Rusmansyah Putra Barat(2), Cut Vela Risqa Handini(2), Dwinna Aliza(3), Amalia Sutriana(1), Hamdan Hamdan(4), Budianto Panjaitan(5), Arman Sayuti(5) and Tongku Nizwan Siregar(4,*)


Pharmacology Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Indonesia

Veterinary Study Program, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Indonesia

Pathology Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Indonesia

Reproduction Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Indonesia

Clinic Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Indonesia

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Abstract

This study aimed to determine the effect of seminal vesicle extract administration to increase the concentration and motility of spermatozoa and testosterone level of kacang goat. The experiment was arranged using a randomly completely block design (RCBD). Three kacang goats were used and each goat received three treatments namely P0 (2 mL of 0.9% NaCl), P1 (1.5 mL Capriglandin, PGF2α), and P2 (2 mL of seminal vesicle extract, EVS). Semen and blood were collected two days post-treatment to measure the concentration and motility of spermatozoa and testosterone level. Testosterone levels were measured with enzyme-linked immunosorbent assay (ELISA). Duration between treatments was one week after semen collection. The result showed that the average spermatozoa concentration (x106) in P0; P1; and P2 respectively were 2,763.0±395.0; 2,060.00±678.2; and 2,387.0±442.7 (P>0.05), while the spermatozoa motility score in P0; P1; and P2 respectively were 3.7±1.1, 3.4±0.5, and 3.4±0.5 (P>0.05). The mean testosterone levels in P0, P1, and P2 respectively were 10.27±5.42, 18.51±19.46, and 29.57±12.96 ng/mL (P<0.05). It can be concluded that administration of EVS did not increase concentration and motility of spermatozoa but increased the level of testosterone in kacang goat.

Keywords: Kacang goat, PGF2α, Seminal vesicle extract, Spermatozoa, Testosterone.

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Cite this paper:
Armansyah, T., Barat, E.R.P., Handini, C.V.R., Aliza, D., Sutriana, A., Hamdan, H., Panjaitan, B., Sayuti, A. and Siregar, T.N. 2018. Concentration and motility of spermatozoa and testosterone level of kacang goat after seminal vesicle extract administration. Open Vet. J. 8(4), 406-410.