Open Veterinary Journal

Peer-Reviewed Journal

 

Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan

A.E. El Tigani-Asil1,*, S.M. EL Sanousi2, M.A. Aljameel3, H. El Beir4, A. Adam5,

M.M. Abdallatif6 and M.E. Hamid7

1Departments of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, Qassim University, Saudi Arabia

2Departments of Microbiology, Faculty of Veterinary Medicine, University of Khartoum, Sudan

3Department of Pathology, Veterinary Research laboratory - Nyala, National Veterinary Research Corporation, Sudan

4Department of Tuberculosis, Institute of Tropical Diseases, Ministry of Science and Technology, Sudan

5Department of Pathology, Faculty of Veterinary Science, University of Nyala, Sudan

6Department of Microbiology, Faculty of Science, North Border University, Saudi Arabia

7Department of Clinical Microbiology and Parasitology, College of Medicine, King Khalid University, Saudi Arabia

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Abstract

This study identified nontuberculous mycobacteria (NTM) recovered from bovine pyogenic affections obtained at necropsy using the molecular target 16S-23S rDNA internal transcribed spacer region. Postmortem inspection of cattle was conducted at South Darfur State abattoirs and Alsabalouga Slaughterhouse at Omdurman area during 2007-2009. Specimens were examined for the presence of acid fast bacteria (AFB) using microscopic and standard culturing techniques. AFB were identified phenotypically and confirmed by 16S-23S rDNA ITS. Fifty nine NTM were recovered and confirmed as acid fast filaments out of 165 positive AFB specimens, of which 52 isolates were identified as bovine farcy causative agents, while 7 cultures were excluded due to drying. 16S-23S rDNA ITS of NTM revealed three different amplicons 500 bp. (32) isolates, 550 bp. (2) isolates and 600 bp. (14) isolates. Four isolates were contaminated.

Keywords: Acid fast bacteria, Bovine farcy, Nontuberculous mycobacteria, Pyogenic infection.

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Cite this paper:

El Tigani-Asil, A.E., EL Sanousi, S.M., Aljameel, M.A., El Beir, H., Adam, A., Abdallatif, M.M. and Hamid, M.E. 2014. Molecular identification of nontuberculous mycobacteria isolated from pyogenic bovine tissues in South Darfur State and Alsabalouga slaughterhouse at Omdurman area, Sudan. Open Vet. J. 4(1), 16-19.