Open Veterinary Journal

Peer-Reviewed Journal

 

Studies on the antidiarrhoeal, antimicrobial and cytotoxic activities of ethanol-extracted leaves of yellow oleander (Thevetia peruviana) 

M.M. Hassan1,*, A.K. Saha1, S.A. Khan1, A. Islam1, M. Mahabub-Uz-Zaman2 and S.S.U. Ahmed3

1Department of Physiology, Biochemistry and Pharmacology, Faculty of Veterinary Medicine, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong-4202, Bangladesh

2Drug and Toxins Research Division, BCSIR Laboratory, Chittagong-4220, Bangladesh

3Department of Medicine and Surgery, Faculty of Veterinary Medicine, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong-4202, Bangladesh

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Abstract

This study screened the antidiarrhoeal, antimicrobial and cytotoxic effects of ethanol-extracted leaves of yellow oleander (Thevetia peruviana). The extract was tested against castor oil-induced diarrhoea in a model of albino rats and showed significant antidiarrhoeal activity (P<0.01). Disc diffusion technique was used to test the in vitro antibacterial activities of the extract and exhibited poor antibacterial activities against both Gram positive and Gram negative bacteria (mainly Bacillus sp). Ethanol-extracted leaves of yellow oleander showed narrow zone of inhibition in the bacterial lawns of Shigella flexineri, Salmonella typhi, Klebsiella sp, Staphylococcus aureus and Shigella sonnei. Cytotoxicty was determined against brine shrimp nauplii and LC50 of the plant extract was determined as 627.21μg/ml.  The wide range of LC50 value denotes the safety effect of the extract.

Keywords: Antidiarrhoeal, Antimicrobial, Cytotoxicity, Albino rats, Brine shrimp, Yellow oleander, LC50

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Cite this paper:

Hassan, M.M., Saha, A.K., Khan, S.A., Islam, A., Mahabub-Uz-Zaman, M. and Ahmed, S.S.U. 2011. Studies on the antidiarrhoeal, antimicrobial and cytotoxic activities of ethanol-extracted leaves of yellow oleander (Thevetia peruviana). Open Vet. J.  1, 28-31