Open Veterinary Journal

Peer-Reviewed Journal

 

Rickettsia rickettsii isolation from naturally infected Amblyomma parvum ticks by centrifugation in a 24-well culture plate technique

K. Dzul-Rosado1, G. Peniche-Lara2, R. Tello-Martín1, J. Zavala-Velázquez2, R. de Campos Pacheco3, M.B. Labruna4, E.C. Sánchez5 and J. Zavala-Castro1,*

1Regional Research Center “Dr. Hideyo Noguchi”, Autonomous University of Yucatan, Mexico

2Faculty of Medicine, Interinstitutional Unit of Clinical and Epidemiological Research. Autonomous University of Yucatan, Mexico

3Federal University of Mato Grosso, Laboratory of Veterinary Parasitology, Department of Basic Science and animal production, Faculty of Agronomy, Animal Medicine and zootechny, Brazil.

4Parasitic Disease laboratory, Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Zootechny, São Paulo University, São Paulo, SP, Brazil

5Government Health Services, Hospital Agustín O´Horan SSY, Calle Itzaes s/n. Col Centro, Merida Yucatan, Mexico

_________________________________________________________________________

Abstract

Rocky Mountain spotted fever is an acute illness caused by Rickettsia rickettsii (R. rickettsii) and is transmitted by the bite of ticks of the genera Dermacentor, Amblyomma and Rhipicephalus. The illness results in a high mortality rate and may be easily confused with other febrile syndromes.  In Yucatan State, Mexico, childhood cases with a high mortality have been reported. In this work we report the isolation of a Mexican R. rickettsii strain from a tick egg mass using an alternative method for Rickettsia isolation with 24-well plates. We also identified a potential vector of R. rickettsii in the southeast of Mexico, which is Amblyomma parvum.

 

Keywords: Amblyomma parvum, Rickettsia rickettsii, Rocky Mountain spotted fever.

_________________________________________________________________________

Cite this paper:

Dzul-Rosado, K., Peniche-Lara, G., Tello-Martín, R., Zavala-Velázquez, J., de Campos Pacheco, R., Labruna, M.B., Sánchez, E.C. and Zavala-Castro, J. 2013. Rickettsia rickettsii isolation from naturally infected Amblyomma parvum ticks by centrifugation in a 24-well culture plate technique. Open Vet. J. 3(2), 101-105.