Open Veterinary Journal

Peer-Reviewed Journal

 

Isolation and molecular identification of Vibrio spp. by sequencing of 16S rDNA from seafood, meat and meat products in Libya

S.M. Azwai1, E.A. Alfallani1, S.K. Abolghait2, A.M. Garbaj3, H.T. Naas3, A.A. Moawad4, F.T. Gammoudi1, H.M. Rayes1, I. Barbieri5 and I.M. Eldaghayes1,*

1Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Tripoli, P.O. Box 13662, Tripoli, Libya

2Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Suez Canal University, 41522 Ismailia, Egypt

3Department of Food Hygiene and Control, Faculty of Veterinary Medicine, University of Tripoli, P.O. Box 13662, Tripoli, Libya

4Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt

5Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, Via Bianchi, 9 - 25124 Brescia, Italy

___________________________________________________________________________

Abstract

The genus Vibrio includes several food-borne pathogens that cause a spectrum of clinical conditions including septicemia, cholera and milder forms of gastroenteritis. Several Vibrio spp. are commonly associated with food-borne transmission including Vibrio cholerae, Vibrio parahemolyticus, and Vibrio vulnificus. Microbiological analysis for enumeration and isolation of Vibrio spp. were carried out for a total of 93 samples of seafood, meat and meat products from different geographic localities in Libya (Tripoli, Regdalin, Janzour and Tobruk). Vibrio spp. were detected by conventional cultural and molecular method using PCR and sequencing of 16S rDNA. Out of the 93 cultured samples only 48 (51.6%) yielded colonies on Thiosulfate Citrate Bile Salt agar (TCBS) with culture characteristics of Vibrio spp. More than half (n=27) of processed seafood samples (n=46) yielded colonies on TCBS, while only 44.6% of samples of meat and meat products showed colonies on TCBS. Among cultured seafood samples, the highest bacterial count was recorded in clam with a count of 3.8 х104 CFU\g. Chicken burger samples showed the highest bacterial count with 6.5 х104 CFU\g. Molecular analysis of the isolates obtained in this study, showed that 11 samples out of 48 (22.9%) were Vibrio spp. Vibrio parahemolyticus was isolated from camel meat for the first time. This study is an initial step to provide a baseline for future molecular research targeting Vibrio spp. foodborne illnesses. This data will be used to provide information on the magnitude of such pathogens in Libyan seafood, meat and meat products.

Keywords: 16S rDNA, Libya, Meat, Seafood, Vibrio.

___________________________________________________________________________

Cite this paper:

Azwai, S.M., Alfallani, E.A., Abolghait, S.K., Garbaj, A.M., Naas, H.T., Moawad, A.A., Gammoudi, F.T., Rayes, H.M., Barbieri, I. and Eldaghayes, I.M. 2016. Isolation and molecular identification of Vibrio spp. by sequencing of 16S rDNA from seafood, meat and meat products in Libya. Open Vet. J. 6(1), 36-43.