Open Veterinary Journal

Peer-Reviewed Journal

 

Comparison of effects of age and sex on serum protein electrophoretic pattern in one-humped camels (Camelus dromedarius) in Semnan, Iran

M. Ahmadi-hamedani1,*, K. Ghazvinian2, P. Kokhaei3,4, M. Barati3 and A. Mahdavi2

1Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran

2Department of Animal Sciences and Food Industries, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran

3Department of Immunology, Semnan University of Medical Sciences, Semnan, Iran

4Immune and Gene Therapy Lab. CCK, Karolinska University Hospital Solna, Stockholm, Sweden

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Abstract

The  aim  of  this  study  was  to  evaluate  the  influence  of  age  and  sex  on  the  concentration  of  total  serum  protein  measured  by  the  biuret  method  and  protein  fractions  determined  using  cellulose  acetate  electrophoresis  in  apparently  healthy  camels  (Camelus  dromedarius).  Blood  samples  were  collected  from  21  camels  (12  males  and  9  females).  The  camels  were  further  divided  into  two  groups:  12  young  camels  at  the  age  of  3  months  to  2  years  and  9  adult  camels  at  the  age  of  3-15  years.  Cellulose  acetate  electrophoresis  of  serum  proteins  identified  five  protein fractions  in  adult  camels  as  young  camels,  these  five  protein  fractions  include    albumin,  α1  and  α2,  β  and  γ-globulins.  In  adult  camels,  serum  levels  (g/l)  of  total  protein,  albumin,  α1-globulins,  α2-globulins,  β-globulins  and  γ-globulins  were  80.9±3.10,  42.9±3.10,  1.3±0.22,  2.2±0.30,  11.8±0.30  and  22.6±0.20,   respectively.  However,  in  young  camels,  these levels  (g/l)  were  66.8±2.90,  40.2±2.40,  1.0±0.14,  2.6±0.30,  10.6±0.80  and  12.3±1.20,  respectively.  The  albumin/globulin  (A/G)  ratio  was  2.08±0.28  in  adult  camels  and 3.77±0.53  in  young  ones. The  mean  serum  concentrations  of  total    protein  and  γ-globulins  were  significantly  (P<0.05)  higher  and  the  A/G  ratio  was  significantly  lower  in  adult  camels  compared  to  young  camels.  The  mean  concentrations  of  γ-globulins  were  significantly  higher  and  the  A/G  ratio  was  significantly  (P<0.05)  lower  in  females   compared  to  male  camels.  The  results  of  the  present  study  indicate  a  significant  effect  of  age  and  sex  on  the  concentrations  of  some of the serum  protein  fractions  in  dromedary  camels.

Keywords: Age,  Camelus dromedarius,  Electrophoresis,  Semnan,  Sex.

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Cite this paper:

Ahmadi-hamedani, M., Ghazvinian, K., Kokhaei, P., Barati, M. and Mahdavi, A. 2014. Comparison of effects of age and sex on serum protein electrophoretic pattern in one-humped camels (Camelus dromedarius) in Semnan, Iran. Open Vet. J. 4(1), 4-8.